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19F-MR Imaging of Nucleic Acids based on Photochemically Hyperpolarlized Nuclear Spins

Research Project

Project/Area Number 24655149
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Chemistry related to living body
Research InstitutionJapan Advanced Institute of Science and Technology

Principal Investigator

FUJIMOTO KENZO  北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 教授 (90293894)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsフッ素 / イメージング / フッ素ラベリング / RNA検出
Research Abstract

In vivo imaging technologies, such as X-ray computed tomography, positron emission tomography, and nuclear magnetic resonance imaging, are promising technologies for diagnosis of various disorders and follow up after surgical treatments. Due to the low background signal of 19 F MR in vivo, these 19F MR based imaging probes have potential for high contrast and specific imaging of target biomolecules. However, molecular 19F probes that can image nucleic acids have been scarcely reported, because of low sensitivity of 19F MR signal. As the method that can tremendously enhance the MR signal of 19F nuclei by using of parahydrogen-induced hyperpolarization (PHIP) of 19F nuclei. So, 19F-MR imaging of DNA based on photochemically generalized parahydrogen caused from cyclobutane dimer by DNA crosslinking was investigated.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (23 results)

All 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (11 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (6 results) (of which Overseas: 1 results)

  • [Journal Article] Details of the ultra-fast DNA photocrosslinking reaction of 3-cyanovinylcarbazole nucleoside; Cis-trans isomeric effect and the application for SNP based genotyping2013

    • Author(s)
      Kenzo Fujimoto, Asuka Yamada, Yoshinaga Yoshimura, Tadashi Tsukaguchi and Takashi Sakamoto
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 135 Issue: 43 Pages: 1616-16167

    • DOI

      10.1021/ja406965f

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Geometric Effect on the Photocrosslinking Reaction between 3-Cyanovinylcarbazole Nucleoside and Pyrimidine Base in DNA/RNA Heteroduplex2013

    • Author(s)
      Kenzo Fujimoto, Satomi Kishi and Takashi Sakamoto
    • Journal Title

      Photochemistry and Photobiology

      Volume: 89 Issue: 5 Pages: 1095-1099

    • DOI

      10.1111/php.12118

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quick and Reversible Photocrosslinking Reaction of 3-Cyanovinylcarbazole Nucleoside in DNA Triplex2013

    • Author(s)
      Kenzo Fujimoto, Hiroki Yoshinaga, Yasumasa Yoshio and Takashi Sakamoto
    • Journal Title

      Organic & Biomolecular Chemistry

      Volume: 11 Issue: 31 Pages: 5065-5068

    • DOI

      10.1039/c3ob40915e

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quick Regulation of mRNA Functions by a Few Seconds of Photoirradiation,2012

    • Author(s)
      Atsuo Shigeno
    • Journal Title

      Organic & Biomolecular Chemistry

      Volume: 10(38) Issue: 38 Pages: 7820-7825

    • DOI

      10.1039/c2ob25883h

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Specific and reversible photochemical labeling of plasmid DNA using photoresponsive oligonucleotides containing 3-cyanovinylcarbazole2012

    • Author(s)
      Kenzo Fujimoto
    • Journal Title

      Molecular BioSystems

      Volume: 8 (2) Issue: 2 Pages: 491-494

    • DOI

      10.1039/c2mb05422a

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 5-Methylcytosine Selective Photoligation Using Photoresponsive, Oligonucleotides Containing Various 5-Vinyl-2’-deoxyuridines Having an Aromatic Group2012

    • Author(s)
      Takashi Sakamoto
    • Journal Title

      Chem. Lett

      Volume: 41(1) Issue: 1 Pages: 47-49

    • DOI

      10.1246/cl.2012.47

    • NAID

      130004426322

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Details of the ultra-fast DNA photocrosslinking reaction of 3-cyanovinylcarbazole nucleoside2013

    • Author(s)
      Takashi Sakamoto, Asuka Yamada, Yoshinaga Yoshimura, Kenzo Fujimoto
    • Organizer
      Internal Symposium of Nucleic Acids Chemistry 2013
    • Place of Presentation
      Kanagawa Univ. (神奈川県)
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of cyanovinylcarbazole mediated photocrosslinking reaction toward for regulation of RNA2013

    • Author(s)
      Atsuo Shigeno ,Takashi Sakamoto, Kenzo Fujimoto
    • Organizer
      40th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Hawaii, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] 超高速光DNA・RNA操作法の開発2013

    • Author(s)
      藤本健造
    • Organizer
      バイオアカデミックフォーラム2013
    • Place of Presentation
      東京ビッグサイト(東京)
    • Related Report
      2013 Final Research Report
  • [Presentation] Details of the ultra-fast DNA photocrosslinking reaction of 3-cyanovinylcarbazole nucleoside2013

    • Author(s)
      Takashi Sakamoto, Asuka Yamada, Yoshinaga Yoshimura and Kenzo Fujimoto
    • Organizer
      International Symosium of Nucleic Acids Chemistry
    • Place of Presentation
      Kanagawa Univ.
    • Related Report
      2013 Annual Research Report
  • [Presentation] 超高速光DNA・RNA操作法の開発2013

    • Author(s)
      藤本 健造
    • Organizer
      バイオアカデミックフォーラム
    • Place of Presentation
      東京ビッグサイト
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of Photochemical DNA and RNA Manipulation2012

    • Author(s)
      Kenzo Fujimoto
    • Organizer
      International Symposium on SYNTHESIZING LIFE AND BIOLOGICAL SYSTEMS
    • Place of Presentation
      SENRI LifeScieence Center(大阪)
    • Related Report
      2013 Final Research Report
  • [Presentation] Development about Photochemical RNA Manipulation2012

    • Author(s)
      Kenzo Fujimoto
    • Organizer
      14th RNA Meeting
    • Place of Presentation
      Tohoku Univ.(宮城県)
    • Related Report
      2013 Final Research Report
  • [Presentation] Template directed DNA photochemical ligation toward for construction of DNA architecure2012

    • Author(s)
      Shigetaka Nakamura, Kenzo Fujimoto
    • Organizer
      13th Tetrahedron Symposium
    • Place of Presentation
      Amsterdam, Nederland
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of Nucleic Acids Based–Probes for the 19F MRI/MRS of Biomolecules2012

    • Author(s)
      Takashi Sakamoto, Hikaru Hayakawa, Jyun Sasaki, Satomi Kishi and Kenzo Fujimoto
    • Organizer
      World Molecular Imaging Congress (WMIC2012)
    • Place of Presentation
      Doublin, Ireland
    • Related Report
      2012 Research-status Report
  • [Presentation] 光化学的なDNA及びRNA操作法の開発2012

    • Author(s)
      藤本 健造
    • Organizer
      関西大学 研究部門発表会
    • Place of Presentation
      関西大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 光を用いたRNA高速操作法の開発2012

    • Author(s)
      藤本 健造
    • Organizer
      バイオアカデミックフォーラム
    • Place of Presentation
      東京ビッグサイト
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 光架橋形成抑制方法、及び自己架橋体形成抑制型光応答性核酸2013

    • Inventor(s)
      藤本健造 中村重孝
    • Industrial Property Rights Holder
      北陸先端科学技術大学院大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-10-30
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 鎖交換された二重鎖オリゴヌクレオチドの製造方法2013

    • Inventor(s)
      藤本健造、中村重孝、橋本浩寿、 小林聡
    • Industrial Property Rights Holder
      北陸先端科学技術大学院大学、電気通信大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-06-25
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 光クロスリンク能を有する光応答性ヌクレオチドアナログ2013

    • Inventor(s)
      藤本健造、坂本隆、田中佑弥
    • Industrial Property Rights Holder
      北陸先端科学技術大学院大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 光クロスリンク能を有する光応答性人工ヌクレオチド2013

    • Inventor(s)
      藤本健造、吉村嘉永
    • Industrial Property Rights Holder
      北陸先端科学技術大学院大学
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2013-05-09
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 光架橋形成抑制方法、及び自己架橋体形成抑制型光応答性核酸2013

    • Inventor(s)
      藤本健造、中村重孝
    • Industrial Property Rights Holder
      北陸先端科学技術大学院大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-225799
    • Filing Date
      2013-10-30
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 光クロスリンク能を有する光応答性ヌクレオチドアナログ2013

    • Inventor(s)
      藤本健造、坂本隆、 田中佑弥
    • Industrial Property Rights Holder
      国立大学法人 北陸先端科学技術大学院大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-070381
    • Filing Date
      2013-03-28
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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