| Project/Area Number |
24655150
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Toyohashi University of Technology |
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Principal Investigator |
UMEKAGE SO 豊橋技術科学大学, 工学(系)研究科(研究院), 講師 (30419436)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | RNA / RNase / 凝集 / RNA医薬 / 核酸 / RNA分解酵素 / ハンマーヘッドリボザイム |
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| Outline of Final Research Achievements |
RNA is well known to be labile against nucleases attack, and it is assumed that using artificial ribonucleotides is inevitable for obtaining nuclease-resistance when preparing RNA therapeutics. In this research, we tried to obtain ribonuclease-resistant RNA transcripts by using only natural ribonucleotides. We found that in vitro transcripted RNA aggregated when meeting some specific conditions and these aggregates obtained both thermostable and ribonuclease-resistance. Also we showed that this aggregation depended on the length of DNA template and RNA contents. These findings will give significant insight into RNA engineering and/or RNA therapeutics when preparing functional RNAs.
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