Development of site-specific conversion method of nucleobase in mRNA

Research Project

Project/Area Number	24655152
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Chemistry related to living body
Research Institution	Osaka University
Principal Investigator	HARI Yoshiyuki 大阪大学, 薬学研究科(研究院), 准教授 (50347423)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	核酸化学
Research Abstract	In this study, we aimed at the development of the method for conversion ofcytosine nucleobase into uracil one in messenger RNA. In order to implement the method, sulnic acid-conjugated oligonucleotides were designed as a new class of modified oligonucleotides, and their syntheses were achieved. Although the conversion reaction of cytosine nucleobase within 2'-O-methyl-RNA using the synthesized oligonucleotides under various conditions was investigated during this research period, we could not succeed in the development of the conversion method. However, this reseach provided useful information for the development of the method in future.

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(3 results)