| Project/Area Number |
24655156
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | Ehime University |
|---|
Principal Investigator |
HORI Hiroyuki 愛媛大学, 理工学研究科, 教授 (20256960)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | ＲＮＡ / RNA modification / methylation / DNA synthesis / folate / enzyme / thermophile / ＲＮＡ ｍｏｄｉｆｉｃａｔｉｏｎ / ｍｅｔｈｙｌａｔｉｏｎ / ＤＮＡ ｓｙｎｔｈｅｓｉｓ / ｆｏｌａｔｅ / ｅｎｚｙｍｅ / ｔｈｅｒｍｏｐｈｉｌｅｓ / RNA修飾 / ＤＮＡ合成 / tRNA / メチル化 / メチル化酵素 / チミジル酸合成酵素 / 葉酸 / 真正細菌 / 酵素 / 核酸 / RNA / ゲノム / 遺伝子 |
|---|
| Outline of Final Research Achievements |
The purpose of this sudy was the validation of our hypothesis, in which the folate-dependent RNA methyltransferase (TrmFO) regulates the DNA synthesis via consumption of 5, 10-methylenetetrahydrofolate. Under nutrient-poor condition, the trmFO gene disruptant strain of extreme-thermophilic eubacterium, Thermus thermophilus, grew faster than the wild-type strain. The slow growth of wild-type strain was accelarated by addition of thymine or thymidilate. These results supported the hypothesis. Furthermore, we determined the molecular ratio of TrmFO, 5, 10-methylenetetrahydrofolate synthase and thymidilate synthase. Our in vitro experiment revealed that TrmFO and thymidilate synthase compete each other via comsumption of 5, 10-methylenetetrahydrofolate.
|
