| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
Ａn important subject in brain disease therapy is in the difficulty of transfer of drugs into the brain. The migration of drugs into the brain is limited by (blood-brain barrier : BBB) to keep the nerve cells in the brain being safe. In this study, I have conducted to develop the nano-body (being several nanometer in size) to pass through the BBB and deliver the drugs into brain.
As shown in the following, 1) the human form nano antibody synthesis, 2) Construction of establishment, 3) permeability test of nano antibodies and complete antibody of using the BBB permeability test kit, etc. were carried out. As the results, monomeric nano-body, dimeric nano-body, and a whole antibody, whose molecular size is, 25kDa, 50kDa, and 150kDa, respectively, showed a small passage through the BBB. The passage efficient through BBB was in the order of the molecular size, 25kDa > 50kDa >150kDa. As the conclusion, nano-size-antibody was effective as a drug to pass the BBB and enter into brain.
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