Preparation of nano-body capable of passing through blood brain barrier (BBB)
| Project/Area Number |
24655158
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Oita University |
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Principal Investigator |
HIFUMI EMI 大分大学, 全学研究推進機構, 教授 (90254606)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | ナノ抗体 / 血液脳関門 / BBB / がん細胞傷害性 / 定常領域ドメイン |
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| Outline of Final Research Achievements |
Ａn important subject in brain disease therapy is in the difficulty of transfer of drugs into the brain. The migration of drugs into the brain is limited by (blood-brain barrier : BBB) to keep the nerve cells in the brain being safe. In this study, I have conducted to develop the nano-body (being several nanometer in size) to pass through the BBB and deliver the drugs into brain.
As shown in the following, 1) the human form nano antibody synthesis, 2) Construction of establishment, 3) permeability test of nano antibodies and complete antibody of using the BBB permeability test kit, etc. were carried out. As the results, monomeric nano-body, dimeric nano-body, and a whole antibody, whose molecular size is, 25kDa, 50kDa, and 150kDa, respectively, showed a small passage through the BBB. The passage efficient through BBB was in the order of the molecular size, 25kDa > 50kDa >150kDa. As the conclusion, nano-size-antibody was effective as a drug to pass the BBB and enter into brain.
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Report
(4 results)
Research Products
(13 results)
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[Presentation] スーパー抗体酵素の発見と展開2014
Author(s)
一二三恵美
Organizer
第24回バイオ・高分子シンポジウム
Place of Presentation
東京工業大学大岡山キャンパス（東京都・目黒区）
Year and Date
2014-07-23 – 2014-07-24
Related Report
Invited
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