Molecular-level in-situ observation of water-ice crystal (melt-crystal) interfaces by developing ultrahigh sensitivity phase contrast microscopy
| Project/Area Number |
24656001
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied materials science/Crystal engineering
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| Research Institution | Hokkaido University |
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Principal Investigator |
SAZAKI GEN 北海道大学, 低温科学研究所, 教授 (60261509)
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| Co-Investigator(Kenkyū-buntansha) |
FURUKAWA Yoshinori 北海道大学, 低温科学研究所, 教授 (20113623)
NAGASHIMA Ken 北海道大学, 低温科学研究所, 助教 (60436079)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 水-氷界面 / 融液-結晶界面 / 分子レベル直接観察 / 超高感度位相差顕微鏡 / 水－氷界面 / 融液－結晶界面 |
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| Research Abstract |
We tried to improve the contrast level of phase-contrast optical microscopy significantly, aiming at molecular-level in-situ observation of melt-crystal interfaces.
We made widths of an orbicular filter (placed at a back focal plane of an objective) and a ring slit 1/4 times thinner (we newly prepared an objective and a ring slit), to decrease the intensity of transmitted light and also to increase the intensity of diffracted light. Then we succeeded in improving the phase-contrast levels of He La cells (standard samples in biology) and water-ice (melt-crystal) interfaces about 2.1 and 1.5 times higher, respectively. These results suggest that the addition of a device that can arbitrarily decrease the intensity of transmitted light can further improve phase contrast level, by perfectly matching the intensities of transmitted and diffracted light.
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Report
(3 results)
Research Products
(28 results)
