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Development of biorelated-material-inspired ionic liquid for a visualizing agent for electron microscopic observations

Research Project

Project/Area Number 24656026
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Thin film/Surface and interfacial physical properties
Research InstitutionHokkaido University

Principal Investigator

YONEZAWA TETSU  北海道大学, 工学(系)研究科(研究院), 教授 (90284538)

Co-Investigator(Kenkyū-buntansha) ABE Shigeaki  北海道大学, 大学院歯学研究科, 助教 (40374566)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords電子顕微鏡 / 走査型電子顕微鏡 / イオン液体 / 可視化 / 導電性 / 生体試料 / 赤血球 / コリン / 細胞
Research Abstract

In this study, we have used ionic liquids as the SEM visualizing reagent of non-conductive nanomaterials and bio-related samples including cells and construct an optimized pre-treatment method for SEM observation. Ionic liquids are organic salts which are in liquid at room temperature and do not evaporate even in a vacuum conditions. Therefore, they should be a suitable material for SEM visualization.
We have been able to establish the processes to visualize non-conductive nanomaterials during this research period. For bio-related samples, including cells, we applied choline-type structured ionic liquids as the visualizing agents. The samples were dispersed in the ionic liquids and put on a membrane filter. The dispersion was filtered gently under reduced pressure by using a syringe and excess ionic liquids were removed. Using this process, red blood cells could be observed by SEM without any deformation.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (47 results)

All 2014 2013 2012 Other

All Journal Article (16 results) (of which Peer Reviewed: 14 results) Presentation (28 results) (of which Invited: 4 results) Remarks (3 results)

  • [Journal Article] Influence of surface properties for SEM observation of carbon nanotubes prepared with room temperature ionic liquid2014

    • Author(s)
      Shigeaki Abe, Atsushi Hyono, and Tetsu Yonezawa
    • Journal Title

      Journal of Solution Chemistry

      Volume: Vol. 43(印刷中)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Static and time lapse observation for estimation of biocompatibility of nano-sized ceramic particles with osteoblasts and hepatocytes2014

    • Author(s)
      Shigeaki Abe, Nobuki Iwadera, Yusuke Hamba, Atsushi Hyono, Fumio Watari, Tsukasa Akasaka, Yasutaka Yawaka, Jun-ichiro Iida, and Tetsu Yonezawa
    • Journal Title

      Biomatter

      Volume: Vol. 4(印刷中)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visualizing preparation using asymmetrical choline-like ionic liquids for scanning electron microscope observation of non-conductive biological samples2014

    • Author(s)
      Shigeaki Abe, Atsushi Hyono, Koji Kawai, and Tetsu Yonezawa
    • Journal Title

      Journal of Nanoscience and Nanotechnology

      Volume: Vol. 14 Pages: 2620-2623

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] SEM observation of the live morphology of human red blood cells under high vacuum conditions using a novel RTIL2014

    • Author(s)
      Atsushi Hyono, Tetsu Yonezawa, Koji Kawai, Shigeaki Abe, Mitsuhiro Fujihara, Hiroshi Azuma, and Shinobu Wakamoto
    • Journal Title

      Surface and Interface Analysis

      Volume: Vol. 46(印刷中) Pages: 425-428

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Influence of surface properties for SEM observation of carbon nanotubes pretreated with Room Temperature Ionic Liquid2014

    • Author(s)
      Shigeaki Abe, Atsushi Hyono, and Tetsu Yonezawa
    • Journal Title

      Journal of Solution Chemistry

      Volume: 43

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Static and time lapse observation for estimation of biocompatibility of nano-sized ceramic particles with osteoblasts and hepatocytes2014

    • Author(s)
      Nobuki Iwadera, Yusuke Hamba, Atsushi Hyono, Shuichi Yamagata, Fumio Watari, Tsukasa Akasaka, Yasutaka Yawaka, Jun-ichiro Iida , Tetsu Yonezawa
    • Journal Title

      Biomatter

      Volume: 4

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualizing preparation using asymmetrical choline-like ionic liquids for SEM observation of non-conductive biological samples2014

    • Author(s)
      S. Abe, A. Hyono, K. Kawai, and T. Yonezawa
    • Journal Title

      J. Nanoscience and Nanotechnology

      Volume: 14 Issue: 3 Pages: 2620-2623

    • DOI

      10.1166/jnn.2014.8629

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SEM observation of the live morphology of human red blood cells under high vacuum conditions using a novel RTIL.2014

    • Author(s)
      A. Hyono, T. Yonezawa, K. Kawai, S. Abe, M. Fujihara, H. Azuma, S. Wakamoto
    • Journal Title

      Surface and Interface Analysis

      Volume: 46 Issue: 6 Pages: 425-428

    • DOI

      10.1002/sia.5471

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct SEM observation of non-electroconductive TiO_2 nanotube arrays prepared by anodization using an ionic liquid as a visualizaing reagent2013

    • Author(s)
      Chaorui Xue, Takashi Narushima, and Tetsu Yonezawa
    • Journal Title

      Journal of Inorganic & Organic Polymers & Materials

      Volume: Vol. 23(印刷中) Pages: 239-242

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simple preatreatment of non-conductive small hydrous bio-samples with choline-type ionic liquid and membrane filter for microsample mounting2013

    • Author(s)
      Koji Kawai, Kotaro Kaneko, Hayato Kawakami, Takashi Narushima, Tetsu Yonezawa
    • Journal Title

      Colloids and Surfaces B–Biointerfaces

      Volume: Vol 102 Pages: 9-12

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] SEM observation of nano carbon materials with imidazolium-type room temperature ionic liquid2013

    • Author(s)
      Shigeaki Abe, Atsushi Hyono, Katsutoshi Nakayama, Tomoya Takada, and Tetsu Yonezawa
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: Vol 52

    • Related Report
      2013 Final Research Report
  • [Journal Article] Direct SEM observation of non-electroconductive TiOF2 nanotube arrays prepared by anodization using an ionic liquid as a visualizing reagent2013

    • Author(s)
      Chaorui Xue, Takashi Narushima, Tetsu Yonezawa
    • Journal Title

      Journal of Inorganic & Organometallic Polymers & Materials

      Volume: 23 Issue: 1 Pages: 239-242

    • DOI

      10.1007/s10904-012-9746-0

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Simple pretreatment of non-conductive small hydrous bio-samples with choline-type ionic liquid and membrane filter for microsample mounting2013

    • Author(s)
      Koji Kawai, Kotaro Kaneko, Hayato Kawakami, Takashi Narushima, Tetsu Yonezawa
    • Journal Title

      Colloids and Surfaces B - Biointerfaces

      Volume: 102 Pages: 9-12

    • DOI

      10.1016/j.colsurfb.2012.08.019

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Scanning electron microscope observation of nano carbon materials with imidazolium-type room temperature ionic liquids2013

    • Author(s)
      S. Abe, A. Hyono, K. Nakayama, T. Takada, and T. Yonezawa
    • Journal Title

      Japanese J. Applied Physics

      Volume: 52 Issue: 1S Pages: 01AH02-01AH02

    • DOI

      10.7567/jjap.52.01ah02

    • NAID

      210000141788

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Conducitivity preparation of choline lactate ethanol solution for SEM observation2012

    • Author(s)
      Shigeaki Abe, Atsushi Hyono, Yuri Machida, Fumio Watari, and Tetsu Yonezawa
    • Journal Title

      Both hard and soft tissues in living matter

      Volume: Vol 4 Pages: 18-23

    • Related Report
      2013 Final Research Report
  • [Journal Article] Conductivity preparation of choline lactate ethanol sotluion for SEM observation: Both hard and soft tissues in living matter2012

    • Author(s)
      Shigeaki Abe, Atsushi Hyono, Yuri Machida, Fumio Watari, and Tetsu Yonezawa
    • Journal Title

      Nano Biomedicine

      Volume: 4 Pages: 18-23

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] イオン液体における応用技術2014

    • Author(s)
      米澤徹
    • Organizer
      ScanTech 2014
    • Place of Presentation
      東京都市大学世田谷キャンパス, 東京都
    • Year and Date
      2014-09-12
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] イオン液体を用いたバイオマテリアルのSEM新規観察法の開発2014

    • Author(s)
      兵野篤, 阿部薫明, 赤坂司, 亘理文夫, 米澤徹
    • Organizer
      代用臓器・再生医学研究会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-03-01
    • Related Report
      2013 Final Research Report
  • [Presentation] Biocompatibility and biodistribution of several micro-/nano-sized inorganic particles2013

    • Author(s)
      Shigeaki Abe, Atsushi Hyono, Yusaku Hamba, Nobuki Iwadera, Shuuichi Yamagata, Fumio Watari, Tsukasa Akasaka, Yasutaka Yawaka, J.-i. Iida, and Tetsu Yonezawa
    • Organizer
      6th International Symposium on Nanotechnology, Occupational and Environmental Health (NanOEH)
    • Place of Presentation
      Nagoya Congress Center, 愛知県
    • Year and Date
      2013-10-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Scanning electron microscope observation of nano/biological materials with room temperature ionic liquids2013

    • Author(s)
      Shigeaki Abe, Atsushi Hyono, Koji Kawai, and Tetsu Yonezawa
    • Organizer
      International Conference on Solution Chemistry
    • Place of Presentation
      Kyoto Tersa, 京都府
    • Year and Date
      2013-07-10
    • Related Report
      2013 Final Research Report
  • [Presentation] イオン液体を用いたナノ/バイオマテリアルのSEM観察2013

    • Author(s)
      阿部薫明, 兵野篤, 河合功治, 米澤徹
    • Organizer
      医学生物学顕微鏡技術学会
    • Place of Presentation
      神奈川歯科大学, 神奈川県
    • Year and Date
      2013-06-08
    • Related Report
      2013 Final Research Report
  • [Presentation] イオン液体による肝細胞由来スフェロイドのSEM観察2013

    • Author(s)
      許斐麻美, 塩野正道, 坂上万里, 中澤英子, 河合功治, 桑畑進, 米澤徹
    • Organizer
      日本顕微鏡学会第69学術講演会
    • Place of Presentation
      ホテル阪急液スポパーク, 大阪府
    • Year and Date
      2013-05-20
    • Related Report
      2013 Final Research Report
  • [Presentation] イオン液体を用いた柔組織のSEM観察2013

    • Author(s)
      坂上万里, 許斐麻美, 塩野正道, 中澤英子, 河合功治, 桑畑進, 米澤徹
    • Organizer
      日本顕微鏡学会第69学術講演会
    • Place of Presentation
      ホテル阪急液スポパーク, 大阪府
    • Year and Date
      2013-05-20
    • Related Report
      2013 Final Research Report
  • [Presentation] SEM観察における生体試料可視化のためのイオン液体の利用2013

    • Author(s)
      阿部薫明, 兵野篤, 河合功治, 亘理文夫, 米澤徹
    • Organizer
      日本顕微鏡学会第69学術講演会
    • Place of Presentation
      ホテル阪急液スポパーク, 大阪府
    • Year and Date
      2013-05-20
    • Related Report
      2013 Final Research Report
  • [Presentation] イオン液体による肝細胞由来スフェロイドのSEM観察2013

    • Author(s)
      許斐麻美,塩野正道,坂上万里,中澤英子,河合功治,桑畑進,米澤徹
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Related Report
      2012 Research-status Report
  • [Presentation] イオン液体を用いた柔組織のSEM観察2013

    • Author(s)
      坂上万里,許斐麻美,塩野正道,中澤英子,河合功治,桑畑進,米澤徹
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Related Report
      2012 Research-status Report
  • [Presentation] SEM観察における生体試料可視化のためのイオン液体の利用2013

    • Author(s)
      阿部薫明,兵野篤,河合功治,亘理文夫,米澤徹
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Related Report
      2012 Research-status Report
  • [Presentation] Double-walled TiO_2 nanotube arrays prepared by anodization in fluoride/glycerol electrolyte2012

    • Author(s)
      Chaorui Xue, Takashi Narushima, and Tetsu Yonezawa
    • Organizer
      2012 International Joint Symposium between HU & CNU on Advanced Material Science and Technology
    • Place of Presentation
      北海道大学, 北海道
    • Year and Date
      2012-11-30
    • Related Report
      2013 Final Research Report
  • [Presentation] イオン液体の電子顕微鏡応用2012

    • Author(s)
      中澤英子, 許斐麻美, 塩野正道, 河合功治, 桑畑進, 米澤徹
    • Organizer
      日本顕微鏡学会第56回シンポジウム
    • Place of Presentation
      北海道大学, 北海道
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] コリン型イオン液体による含水物質への導電性付与とSEM観察への応用2012

    • Author(s)
      米澤徹
    • Organizer
      日本顕微鏡学会第56回シンポジウム
    • Place of Presentation
      北海道大学, 北海道
    • Related Report
      2013 Final Research Report
  • [Presentation] チタニアナノチューブアレーの簡便なSEM観察法2012

    • Author(s)
      薜超瑞, 成島隆, 米澤徹
    • Organizer
      日本顕微鏡学会第68回学術講演会
    • Place of Presentation
      つくば国際会議場, 茨城県
    • Related Report
      2013 Final Research Report
  • [Presentation] イオン液体を用いたナノカーボン物質の微細構造の観察2012

    • Author(s)
      阿部薫明, 兵野篤, 亘理文夫, 米澤徹
    • Organizer
      日本顕微鏡学会第68回学術講演会
    • Place of Presentation
      つくば国際会議場, 茨城県
    • Related Report
      2013 Final Research Report
  • [Presentation] イオン液体の電子顕微鏡応用2012

    • Author(s)
      中澤英子,甲斐麻美,塩野正道,河合功治,桑畑 進,米澤 徹
    • Organizer
      日本顕微鏡学会第56回シンポジウム
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] コリン型イオン液体による含水物質への導電性付与とSEM観察への応用2012

    • Author(s)
      米澤 徹
    • Organizer
      日本顕微鏡学会第56回シンポジウム
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2012 Research-status Report
  • [Presentation] チタニアナノチューブアレーの簡便なSEM観察法2012

    • Author(s)
      薛 超瑞,成島 隆,米澤 徹
    • Organizer
      日本顕微鏡学会第68回学術講演会
    • Place of Presentation
      つくば国際会議場(茨城県)
    • Related Report
      2012 Research-status Report
  • [Presentation] イオン液体を用いたナノカーボン物質の微細構造の観察2012

    • Author(s)
      阿部薫明,兵野 篤,亘理文夫,米澤 徹
    • Organizer
      日本顕微鏡学会第68回学術講演会
    • Place of Presentation
      つくば国際会議場(茨城県)
    • Related Report
      2012 Research-status Report
  • [Presentation] SEM観察における生体試料可視化のためのイオン液体の利用

    • Author(s)
      阿部薫明、兵野篤、河合功治、亘理文夫、米澤徹
    • Organizer
      日本電子顕微鏡学会
    • Place of Presentation
      ホテル阪急エキスポパーク(吹田市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] イオン液体を用いたナノ/バイオマテリアルのSEM観察

    • Author(s)
      阿部薫明、兵野篤、河合功治、米澤徹
    • Organizer
      医学生物学顕微鏡技術学会
    • Place of Presentation
      神奈川歯科大学(横須賀市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Scanning Electron Microscope observation of nano/ biological materials with Room Temperature Ionic Liquids

    • Author(s)
      Shigeaki ABE, Atsushi Hyono, KOji Kawai, and Tetsu Yonezawa
    • Organizer
      International Conferene on Solution Chemistry
    • Place of Presentation
      Kyoto Tersa (京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Biocompatibility and their biodistribution of several nano-sized metal / metal oxide particles

    • Author(s)
      Shigeaki ABE, Atsushi HYONO, Yuusaku HAMBA, Nobuki IWADERA, Shuuichi YAMAGATA, Fumio WATARI, Tsukasa AKASAKAk, Yasutaka YAWAKA, J.-i. IIDA and Tetsu YONEZAWA
    • Organizer
      International Symposium on Surface and Interface Biomaterials
    • Place of Presentation
      Roma University (イタリア、ローマ)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Biocompatibility and biodistribution of several micro-/nano-sized inorganic particles

    • Author(s)
      Shigeaki ABE, Atsushi Hyono, Yusaku Hamba, Nobuki Iwadera, Shuuichi Yamagata, Fumio Watari, Tsukasa Akasaka, Yasutaka Yawaka, J.-i. Iida and Tetsu Yonezawa
    • Organizer
      NanOEH
    • Place of Presentation
      Nagoya Congress Center (名古屋市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] イオン液体を用いたバイオマテリアルのSEM新規観察法の開発

    • Author(s)
      兵野 篤, 阿部 薫明, 赤坂司, 亘理文夫, 米澤 徹
    • Organizer
      代用臓器・再生医学研究会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] イオン液体における応用技術

    • Author(s)
      米澤徹
    • Organizer
      SCAN TECH 2014
    • Place of Presentation
      東京都市大学 世田谷キャンパス(東京都)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] コリン型イオン液体による含水物質への導電性付与とSEM観察への応用

    • Author(s)
      Chaorui Xue, Takashi Narushima, Tetsu Yonezawa
    • Organizer
      2012 International Joint Symposium between HU & CNU on Advanced Material Science and Technology
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2012 Research-status Report
  • [Remarks] 先進材料ハイブリッド工学研究室ホームページ

    • URL

      http://labs.eng.hokudai.ac.jp/labo/limsa/

    • Related Report
      2013 Final Research Report
  • [Remarks] 先進材料ハイブリッド工学研究室

    • URL

      http://labs.eng.hokudai.ac.jp/labo/limsa/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 表界面微細構造研究室

    • URL

      http://www.eng.hokudai.ac.jp/labo/lims/

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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