Development of biorelated-material-inspired ionic liquid for a visualizing agent for electron microscopic observations
| Project/Area Number |
24656026
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Thin film/Surface and interfacial physical properties
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| Research Institution | Hokkaido University |
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Principal Investigator |
YONEZAWA TETSU 北海道大学, 工学(系)研究科(研究院), 教授 (90284538)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Shigeaki 北海道大学, 大学院歯学研究科, 助教 (40374566)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 電子顕微鏡 / 走査型電子顕微鏡 / イオン液体 / 可視化 / 導電性 / 生体試料 / 赤血球 / コリン / 細胞 |
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| Research Abstract |
In this study, we have used ionic liquids as the SEM visualizing reagent of non-conductive nanomaterials and bio-related samples including cells and construct an optimized pre-treatment method for SEM observation. Ionic liquids are organic salts which are in liquid at room temperature and do not evaporate even in a vacuum conditions. Therefore, they should be a suitable material for SEM visualization.
We have been able to establish the processes to visualize non-conductive nanomaterials during this research period. For bio-related samples, including cells, we applied choline-type structured ionic liquids as the visualizing agents. The samples were dispersed in the ionic liquids and put on a membrane filter. The dispersion was filtered gently under reduced pressure by using a syringe and excess ionic liquids were removed. Using this process, red blood cells could be observed by SEM without any deformation.
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Report
(3 results)
Research Products
(47 results)
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[Presentation] Biocompatibility and biodistribution of several micro-/nano-sized inorganic particles2013
Author(s)
Shigeaki Abe, Atsushi Hyono, Yusaku Hamba, Nobuki Iwadera, Shuuichi Yamagata, Fumio Watari, Tsukasa Akasaka, Yasutaka Yawaka, J.-i. Iida, and Tetsu Yonezawa
Organizer
6th International Symposium on Nanotechnology, Occupational and Environmental Health (NanOEH)
Place of Presentation
Nagoya Congress Center, 愛知県
Year and Date
2013-10-29
Related Report
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[Presentation] イオン液体を用いた柔組織のSEM観察2013
Author(s)
坂上万里, 許斐麻美, 塩野正道, 中澤英子, 河合功治, 桑畑進, 米澤徹
Organizer
日本顕微鏡学会第69学術講演会
Place of Presentation
ホテル阪急液スポパーク, 大阪府
Year and Date
2013-05-20
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[Presentation] コリン型イオン液体による含水物質への導電性付与とSEM観察への応用
Author(s)
Chaorui Xue, Takashi Narushima, Tetsu Yonezawa
Organizer
2012 International Joint Symposium between HU & CNU on Advanced Material Science and Technology
Place of Presentation
北海道大学（北海道）
Related Report
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