| Project/Area Number |
24656026
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Thin film/Surface and interfacial physical properties
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| Research Institution | Hokkaido University |
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Principal Investigator |
YONEZAWA TETSU 北海道大学, 工学(系)研究科(研究院), 教授 (90284538)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Shigeaki 北海道大学, 大学院歯学研究科, 助教 (40374566)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 電子顕微鏡 / 走査型電子顕微鏡 / イオン液体 / 可視化 / 導電性 / 生体試料 / 赤血球 / コリン / 細胞 |
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| Research Abstract |
In this study, we have used ionic liquids as the SEM visualizing reagent of non-conductive nanomaterials and bio-related samples including cells and construct an optimized pre-treatment method for SEM observation. Ionic liquids are organic salts which are in liquid at room temperature and do not evaporate even in a vacuum conditions. Therefore, they should be a suitable material for SEM visualization.
We have been able to establish the processes to visualize non-conductive nanomaterials during this research period. For bio-related samples, including cells, we applied choline-type structured ionic liquids as the visualizing agents. The samples were dispersed in the ionic liquids and put on a membrane filter. The dispersion was filtered gently under reduced pressure by using a syringe and excess ionic liquids were removed. Using this process, red blood cells could be observed by SEM without any deformation.
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