Study on the mechanism of tissue disruption induced by decreased expression of cell-cell adhesion proteins
| Project/Area Number |
24656175
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
AOMURA Shigeru 首都大学東京, システムデザイン研究科, 教授 (20281248)
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| Co-Investigator(Kenkyū-buntansha) |
HUJIWARA Satoshi 横浜市立大学, 生体防御医学研究所, 教授 (20173487)
KAKUTA Akira 東京工業高等専門学校, その他部局等, 准教授 (60224359)
NAKADATE Hiromichi 首都大学東京, システムデザイン研究科, 助教 (10514987)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 毛細血管 / 細胞間接着タンパク質 / 衝撃圧力 / 組織崩壊 / 脳内点状出血 / 頭部外傷 / 脳挫傷 / 血管内皮細胞 / 経内皮電気抵抗値 / 血管透過性 / 密着結合 |
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| Research Abstract |
Intracranial pressure changes during head impact cause brain injuries such as vasogenic edema and cerebral contusion.Human umbilical vein endothelial cells were subjected to positive and negative pressure, and the transendothelial electrical resistance (TEER) was measured between 15 min and 24 h after pressure loading for quantifying the formation of an integral monolayer of endothelial cells.
TEER decreased considerably at 15 min and 6 h post-loading, with these changes being significant in positive pressure with larger amplitude and shorter duration and in all types of negative pressures compared to pre-loading.The changes in intracranial pressure during head impact impair endothelial barrier function by the disruption of the integrity of endothelial cell-cell junctions, and the degree of increase in endothelial permeability depends on the amplitude, duration, and direction (compressive and tensile) of the impulsive pressure.
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Report
(3 results)
Research Products
(15 results)
