High-sensitive biochemical gas sensor based on the gas-biomolecule interaction

• Project/Area Number: 24656252
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Measurement engineering
• Research Institution: Meiji University (2013); Tokyo Medical and Dental University (2012)
• Principal Investigator: KUDO Hiroyuki 明治大学, 理工学部, 准教授 (70329118)
• Co-Investigator(Kenkyū-buntansha): MITSUBAYASHI Kohji 東京医科歯科大学, 生体材料工学研究所, 教授 (40307236) ; ARAKAWA Takahiro 東京医科歯科大学, 生体材料工学研究所, 講師 (50409637)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: バイオセンサ / ガスセンサ / 環境計測 / 生化学

Research Abstract

A biosensor using NADH as an electron acceptor was developed. The biosensor measures the presence of the substrate as fluorescence of NADH. In order to enhance the sensitivity to formaldehyde, chemical amplification of NADH was investigated. Aldehyde dehydrogenase produces formic acid and NADH by oxidizing formaldehyde under the presence of NAD+. We co-immobilized formic acid dehydrogenase to re-produce NADH using formic acid. Consequently, the sensor showed sub-ppb level sensitivity to forimaldehyde. Also, real-sample test using photocatalystic TiO2 was carried out. The sensor successfully traced the change of formaldehyde by the effect of photocatalystic reaction in real-time.

Research Products

• [Journal Article] Fiber-optic Fluoroimmunoassay for Determination of Dermatophagoides farinae Allergen by Flow Analysis Technique (2013)
  • Author(s): Miyajima K, Miki D, Arakawa T, Kudo H, Saito H, Mitsubayashi K
  • Journal Title: Sensors and Materials Volume: 25(9) Pages: 591-599
• [Journal Article] NADH-fluorometric Biochemical Gas sensor (bio-sniffer) for Evaluation of Indoor Air Quality (2013)
  • Author(s): Kudo H, Yamashita T, Miyajima K, Arakawa T, Mitsubayashi K
  • Journal Title: IEEE Sensors Journal Volume: vol.13, No.8 Pages: 2828-2833
• [Journal Article] A direct gaseous ethanol imaging system for analysis of alcohol metabolism from exhaled breath (2013)
  • Author(s): Arakawa T, Wang X, Kajiro K, Miyajima K, Takeuchi S, Kudo H, Yano K, Mitsubayashi K
  • Journal Title: Sensors and Actuators B : Chemical Volume: 186 Pages: 27-33
• [Journal Article] A direct gaseous ethanol imaging system for analysis of alcohol metabolism from exhaled breath (2013)
  • Author(s): Arakawa T, Wang X, Kajiro K, Miyajima K, Takeuchi S, Kudo H, Yano K, Mitsubayashi K
  • Journal Title: Sensors and Actuators B: Chemical Volume: Vol. 186 Pages: 27-33
  • DOI: 10.1016/j.snb.2013.05.071
  • Peer Reviewed
• [Journal Article] Fiber-optic biochemical gas sensor (bio-sniffer) for sub-ppb monitoring of formaldehyde vapor (2012)
  • Author(s): Kudo H, Wang X, Suzuki Y, Ye M, Yamashita T, Gessei T, Miyajima K, Arakawa T, Mitsubayashi K
  • Journal Title: Sems. Actuator-B Pages: 161-166
• [Journal Article] A highky sensitive and temporal visualization system for gaseous ethanol chemiluminescence enhancer (2012)
  • Author(s): Arakawa T. Ando E. Wang X, Miyajima K, Kudo H, Saito H, Mitani T, Takahashi M, Mitsubayashi K
  • Journal Title: Luminescence Volume: 27 Pages: 328-333
• [Journal Article] Fiber-optic biochemical gas sensor (bio-sniffer) for sub-ppb monitoring of formaldehyde vapor (2012)
  • Author(s): Kudo H, Wang X, Suzuki Y, Ye M, Yamashita T, Gessei T, Miyajima K, Arakawa T, Mitsubayashi K
  • Journal Title: Sens.Actuators B Chem Volume: 161 Issue: 1 Pages: 486-492
  • DOI: 10.1016/j.snb.2011.10.065
  • Peer Reviewed
• [Journal Article] A highly sensitive and temporal visualization system for gaseous ethano l with chemiluminescence enhancer (2012)
  • Author(s): Arakawa I, Ando E, Wang X, Miyajima K, Kudo H, Saito H, Mitani T, Takahashi M, Mitsubayashi K
  • Journal Title: Luminescence Volume: 27 Issue: 5 Pages: 328-333
  • DOI: 10.1002/bio.1352
  • Peer Reviewed
• [Presentation] Real-time bio-imaging system of breath ethanol after drinking by optical bio-sniffer techniques (2013)
  • Author(s): Wang X, Kita K, Miyajima K, Arakawa T, Kudo H, Mitsubayashi K
  • Organizer: 3rd International Conference on Bio-sensing Technology
  • Place of Presentation: Sitges, Spain
• [Presentation] A highly sensitive formaldehyde gas sensor “bio-sniffer” for monitoring of indoor environment (2013)
  • Author(s): Ye M, Yamashita T, Gessei T, Miyajima K, Munkhjargal M, Arakawa T, Kudo H, Mitsubayashi K
  • Organizer: 12th Asian Conference on Analytical Sciences (ASIANALYSIS XII)
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Application of chemiluminescence involving flow injection analysis to determination of glucose, glutamic acid and lactic acid for food analysis (2013)
  • Author(s): Wang X, Ishii N, Ye M, Munkhjargal M, Miyajima K, Arakawa T, Saito H, Kudo H, Endo H, Mitsubayashi K
  • Organizer: IEEE SENSORS 2013
  • Place of Presentation: Baltimore, Maryland, USA
• [Presentation] アルコール代謝機能評価のための呼気中エタノールガスの可視化計測システムに関する研究 (2013)
  • Author(s): 荒川貴博，王昕，神白匠，ムンフジャルガルムンフバヤル，宮島久美子，工藤寛之，矢野和義，三林浩二
  • Organizer: 電気学会センサ・マイクロマシン部門 第30回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 仙台
• [Presentation] NADH-fluorometric biochemical gas sensor (bio-sniffer) for control of indoor air quality (2012)
  • Author(s): Kudo H, Yamashita T, Gessei T, Arakawa T, Mitsubayashi K
  • Organizer: 22nd Anniversary World Congress on Biosensors (Biosensors 2012)
  • Place of Presentation: Cancun, Mexico
• [Presentation] NADH-fluorometric biochemical gas sensor (bio-sniffer) for assessment of indoor air quality (2012)
  • Author(s): Kudo H, Yamashita T, Gessei T, Ye M, Miyajima K, Arakawa T, Mitsubayashi K
  • Organizer: IEEE SENSORS 2012
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Fiber-optic fluorometric biochemical gas sensor for monitoring of indoor formaldehyde at sub-ppb level (2012)
  • Author(s): Kudo H, Yamashita T, Ye M, Miyajima K, Gessei T, Arakawa T, Mitsubayashi K
  • Organizer: Europt(r)ode XI
  • Place of Presentation: Barcelona, Spain
• [Presentation] 先天性アミノ酸代謝異常症評価のためのNADH蛍光検出型バイオセンサ (2012)
  • Author(s): 工藤寛之, 越田智之, 宮島久美子, 荒川貴博, 矢野和義, 三林浩二
  • Organizer: 第29回「センサ・マイクロマシンと応用システム」シンポジウム
• [Presentation] 環境中の極微量ホルムアルデヒド計測を目的としたNADH蛍光検出型生化学式ガスセンサ (2012)
  • Author(s): 工藤寛之, 山下俊文, 叶明, 宮島久美子, 荒川貴博, 関口哲志, 三林浩二
  • Organizer: 日本バイオマテリアル学会シンポジウム2012
  • Place of Presentation: 仙台
• [Presentation] 先天性アミノ酸代謝異常症評価のためのNADH蛍光検出型バイオセンサ (2012)
  • Author(s): 工藤寛之，越田智之，宮島久美子，荒川 貴博，矢野 和義，三林 浩二
  • Organizer: 第29回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 北九州市
• [Presentation] 環境中の極微量ホルムアルデヒド計測を目的としたNADH蛍光検出型生化学式ガスセンサ (2012)
  • Author(s): 工藤寛之，山下俊文，叶明，宮島久美子，荒川貴博，関口哲志，三林浩二
  • Organizer: 日本バイオマテリアル学会シンポジウム2012
  • Place of Presentation: 仙台市
• [Presentation] Real-time bio-imaging system of breath ethanol after drinking by optical bio-sniffer techniques
  • Author(s): Wang X, Kita K, Miyajima K, Arakawa T, Kudo H, Mitsubayashi K
  • Organizer: 3^<rd> Int. Conf. Bio-sensing Technology
  • Place of Presentation: Sitges, Spain
• [Presentation] A highly sensitive formaldehyde gas sensor"bio-sniffer"for monitoring of indoor environment
  • Author(s): Ye M, Yamashita T, Gessei T, Miyajima K, Munkhjargal M, Arakawa T, Kudo H, Mitsubayashi K
  • Organizer: 12^<th> Asian Conf. Anal. Sciences
  • Place of Presentation: Fukuoka, Japan