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Development of a rapid and simple method based on fluorescence polarization for quantification of waterborne viruses

Research Project

Project/Area Number 24656307
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Civil and environmental engineering
Research InstitutionHokkaido University

Principal Investigator

SHIRASAKI NOBUTAKA  北海道大学, 工学(系)研究科(研究院), 助教 (60604692)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords水系感染症 / ノロウイルス / 蛍光偏光法 / カプシドタンパク質 / 抗原抗体反応 / 金コロイド / 3DNA / 複合体 / ウイルス / 新規ウイルス定量法 / 標識化 / MALDI-TOF-MS
Research Abstract

The objective of this study was to develop a rapid and simple method applying fluorescence polarization which has been used as a tool to evaluate protein-protein interaction for quantification of waterborne viruses. As a result, novel method that can detect norovirus particles within 30 min was successfully developed based on the investigation of effects of plate type, antibody type, antibody concentration and antigen-antibody reaction time on virus detection by fluorescence polarization.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2013 2012 Other

All Presentation (10 results)

  • [Presentation] ウイルス様粒子とImmuno-PCR法を併用したノロウイルスの膜ろ過処理性評価及び処理メカニズムの解明2013

    • Author(s)
      白崎伸隆,田附雄一,松下拓,松井佳彦
    • Organizer
      第50回環境工学研究フォーラム
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2013-11-20
    • Related Report
      2013 Final Research Report
  • [Presentation] インライン凝集-セラミック膜ろ過処理によるノロウイルス粒子の効果的除去2013

    • Author(s)
      白崎伸隆,田附雄一,松下拓,松井佳彦
    • Organizer
      土木学会平成25年度全国大会,第68回年次学術講演会
    • Place of Presentation
      千葉県習志野市
    • Year and Date
      2013-09-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Estimating norovirus removal performance in an in-line coagulation–ceramic microfiltration process by using recombinant norovirus VLPs and immuno-PCR method2013

    • Author(s)
      Shirasaki, N., Matsushita, T., Tatsuki, Y. and Matsui, Y
    • Organizer
      7th IWA Specialised Membrane Technology Conference and Exhibition for Water and Wastewater Treatment and Reuse
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2013-08-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Virus removal by a coagulation–microfiltration process. The 4th IWA Asia-Pacific Young Water Professionals Conference 20122012

    • Author(s)
      Shirasaki, N., Matsushita, T. and Matsui, Y
    • Organizer
      Health-Related Water Microbiology Special Session
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2012-12-08
    • Related Report
      2013 Final Research Report
  • [Presentation] ウイルス様粒子と新規イムノPCR法を併用したヒトノロウイルスの膜ろ過処理性評価2012

    • Author(s)
      白崎伸隆,田附雄一,松下拓,松井佳彦
    • Organizer
      土木学会平成24年度全国大会,第67回年次学術講演会
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2012-09-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Virus removal by a coagulation-microfiltration process2012

    • Author(s)
      Shirasaki, N., Matsushita, T. and Matsui, Y.
    • Organizer
      The 4th IWA Asia-Pacific Young Water Professionals Conference 2012
    • Place of Presentation
      Miraikan, Tokyo, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] ウイルス様粒子と新規イムノPCR法を併用したヒトノロウイルスの膜ろ過処理性評価2012

    • Author(s)
      白崎伸隆, 田附雄一, 松下拓, 松井佳彦
    • Organizer
      土木学会平成24年度全国大会, 第67回年次学術講演会
    • Place of Presentation
      名古屋大学, 愛知県名古屋市
    • Related Report
      2012 Research-status Report
  • [Presentation] Estimating norovirus removal performance in an in-line coagulation-ceramic microfiltration process by using recombinant norovirus VLPs and immuno-PCR method

    • Author(s)
      Shirasaki, N., Matsushita, T., Tatsuki, Y. and Matsui, Y.
    • Organizer
      7th IWA Specialised Membrane Technology Conference and Exhibition for Water and Wastewater Treatment and Reuse
    • Place of Presentation
      Westin Harbour Castle, Toronto, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] ウイルス様粒子とImmuno-PCR法を併用したノロウイルスの膜ろ過処理性評価及び処理メカニズムの解明

    • Author(s)
      白崎伸隆, 田附雄一, 松下拓, 松井佳彦
    • Organizer
      第50回環境工学研究フォーラム
    • Place of Presentation
      北海道大学, 北海道札幌市
    • Related Report
      2013 Annual Research Report
  • [Presentation] インライン凝集-セラミック膜ろ過処理によるノロウイルス粒子の効果的除去

    • Author(s)
      白崎伸隆, 田附雄一, 松下拓, 松井佳彦
    • Organizer
      土木学会平成25年度全国大会, 第68回年次学術講演会
    • Place of Presentation
      日本大学, 千葉県習志野市
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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