Development of a rapid and simple method based on fluorescence polarization for quantification of waterborne viruses
| Project/Area Number |
24656307
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Civil and environmental engineering
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 水系感染症 / ノロウイルス / 蛍光偏光法 / カプシドタンパク質 / 抗原抗体反応 / 金コロイド / 3DNA / 複合体 / ウイルス / 新規ウイルス定量法 / 標識化 / MALDI-TOF-MS |
|---|
| Research Abstract |
The objective of this study was to develop a rapid and simple method applying fluorescence polarization which has been used as a tool to evaluate protein-protein interaction for quantification of waterborne viruses. As a result, novel method that can detect norovirus particles within 30 min was successfully developed based on the investigation of effects of plate type, antibody type, antibody concentration and antigen-antibody reaction time on virus detection by fluorescence polarization.
|
Report
(3 results)
Research Products
(10 results)
