Development of a novel RNA detection method for understanding environmental microbial ecology

Research Project

Project/Area Number	24656309
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Civil and environmental engineering
Research Institution	Tohoku University
Principal Investigator	HARADA Hideki 東北大学, 工学(系)研究科(研究院), 教授 (70134971)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	核酸定量
Outline of Final Research Achievements	A novel RNA detection and quantification method using molecular weight cut-off membrane was developed for deciphering microbial population dynamics in environmental biotechnologies. After demonstrating the proof-of-concept of the method, an experimental protocol and an optimization method of hybridization stringency for specific detection were established. Eventually, some members involved in anaerobic wastewater treatment processes were detected and quantified by the originally developed method.

Report

(4 results)