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Design of Nano-reactor Programmed by Synthesized DNA

Research Project

Project/Area Number 24656472
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Properties in chemical engineering process/Transfer operation/Unit operation
Research InstitutionKyushu University

Principal Investigator

GOTO Masahiro  九州大学, 工学(系)研究科(研究院), 教授 (10211921)

Co-Investigator(Kenkyū-buntansha) KUBOTA Fukiko  九州大学, 工学研究院, 助教 (60294899)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsナノリアクター / アプタマー / 自己組織化 / タンパク質 / DNA / ナノテクノロジー / バイオ材料 / 分子集合体 / 融合タンパク質 / ナノマテリアル / 生体分子
Research Abstract

We present the covalent protein-protein conjugated nanoreactor, which is programmed by synthesized DNA. To demonstrate the concept, we chose thrombin as a model protein for the protein conjugation and designed four different single-stranded DNA. One of them is a template of a DNA scaffold for the arrangement of the thrombin-binding aptamers and three of them are thrombin-binding aptamers with different sticky ends that can hybridize with the DNA template (aptamer unit). By self-assembling these oligonucleotides, they should form a comb-like structure (termed DNA scaffold) composed of both the linear chains due to the DNA double-strands and the branched arms (the flexible DNA aptamer units). Thrombin molecules can get close to each other by the binding of the thrombin molecules with the aptamer units. Then, the addition of a chemical cross-linker induces the neighboring thrombin molecules on the DNA scaffold to easily cross-link each other.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (27 results)

All 2014 2013 2012

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (19 results) (of which Invited: 2 results)

  • [Journal Article] Protein supramolecular complex formation by site-specific avidin biotin interactions2013

    • Author(s)
      Y. Mori, R. Wakabayashi, M. Goto, N. Kamiya
    • Journal Title

      Org. Biomol. Chem.

      Volume: 11 Pages: 914-922

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tailing DNA aptamers with functional proteins by two-step enzymatic reaction2013

    • Author(s)
      M. Takahara, K. Hayashi, M. Goto, N. Kamiya
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 116 Pages: 660-665

    • NAID

      110009687827

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tailing DNA aptamers with functional proteins by two-step enzymatic reaction2013

    • Author(s)
      M. TAKAHARA, K. HAYASHI, M. GOTO, N. KAMIYA
    • Journal Title

      J. Biosci. Bioeng

      Volume: 116 Issue: 6 Pages: 660-665

    • DOI

      10.1016/j.jbiosc.2013.05.025

    • NAID

      110009687827

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microplate assay for aptamer-based thrombin detection using a DNA-enzyme conjugate based on His-tag chemistry2012

    • Author(s)
      J. Shimada, T. Maruyama, M. Kitaoka, N. Kamiya, M. Goto
    • Journal Title

      Anal. Biochem.

      Volume: 421 Pages: 541-546

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Programmable protein protein conjugation via DNA-based self-assembly2012

    • Author(s)
      J. Shimada, T. Maruyama, M. Kitaoka, H. Yoshinaga, K. Nakano, N. Kamiya and M. Goto
    • Journal Title

      Chem. Commun.

      Volume: 48 Pages: 6226-6228

    • NAID

      120006318790

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transglutaminase-mediated in situ hybridization (TransISH) system : A new methodology for simplified mRNA detection2012

    • Author(s)
      M. Kitaoka, M. Mitsumori, K. Hayashi, Y. Hiraishi, H. Yoshinaga, K. Nakano, K. Miyawaki, S. Noji, M. Goto, N. Kamiya
    • Journal Title

      Anal. Chem.

      Volume: 84 Pages: 5885-5891

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Control of a tyrosyl radical mediated protein cross-linking reaction by electrostatic interaction2012

    • Author(s)
      K. Minamihata, M. Goto, N. Kamiya
    • Journal Title

      Bioconjugate Chem.

      Volume: 23 Pages: 1600-1609

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transglutaminase-mediated in situ hybridization (TransISH) system: A new methodology for simplified mRNA detection.2012

    • Author(s)
      M. Kitaoka, M. Mitsumori, K. Hayashi, Y. Hiraishi, H. Yoshinaga, K. Nakano, K. Miyawaki, S. Noji, M. Goto, N. Kamiya
    • Journal Title

      Anal. Chem.

      Volume: 84 Issue: 14 Pages: 5885-5891

    • DOI

      10.1021/ac2034198

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Directed Aggregation and Fusion of Vesicles Induced by DNA Surfactants2014

    • Author(s)
      M. Goto
    • Organizer
      AIChE Meeting
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] ペプチド集合体の形態制御と細胞培養基材への展開2013

    • Author(s)
      古賀未佳, 若林里衣, 神谷典穂, 後藤雅宏
    • Organizer
      第50回化学関連支部合同九州大会
    • Related Report
      2013 Final Research Report
  • [Presentation] ペプチド集合体のナノ構造制御と三次元細胞培養基材への応用2013

    • Author(s)
      若林里衣, 古賀未佳, 神谷典穂, 後藤雅宏
    • Organizer
      日本化学会第93回春季年会
    • Related Report
      2013 Final Research Report
  • [Presentation] Fabrication of Higher-Order Protein Supramolecular Complexes2013

    • Author(s)
      Y. Mori, R. Wakabayashi, M. Goto, N. Kamiya
    • Organizer
      IGER International Symposium on Cell Surface Structures and Functions
    • Related Report
      2013 Final Research Report
  • [Presentation] 自己集合ペプチドのナノ構造制御と細胞培養基材への応用2013

    • Author(s)
      若林里衣,, 古賀未佳, 神谷典穂, 後藤雅宏
    • Organizer
      化学工学会第45回秋季大会
    • Related Report
      2013 Final Research Report
  • [Presentation] 酵素応答性ペプチドハイドロゲルのネットワーク形成制御2013

    • Author(s)
      古賀未佳, 若林里衣, 神谷典穂, 後藤雅宏
    • Organizer
      日本化学会第7回バイオ関連化学シンポジウム
    • Related Report
      2013 Final Research Report
  • [Presentation] Nanostructured Peptide Amphiphile Assemblies for Bioactivity Control2013

    • Author(s)
      R. Wakabayashi, M. Koga, N. Kamiya, M. Goto
    • Organizer
      8th International Symposium on Macrocyclic and Supramolecular Chemistry (8-ISMSC)
    • Related Report
      2013 Final Research Report
  • [Presentation] 自己集合ペプチドのナノ構造制御と細胞培養基材への応用2013

    • Author(s)
      若林里衣、古賀未佳、神谷典穂、後藤雅宏
    • Organizer
      化学工学会第45回秋季大会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 両親媒性ペプチドから形成されるナノ構造の形態が細胞機能に及ぼす影響2013

    • Author(s)
      古賀未佳、若林里衣、神谷典穂、後藤雅宏
    • Organizer
      化学工学会第45回秋季大会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ペプチド超分子集合体の新規ナノ構造制御2013

    • Author(s)
      若林里衣、古賀未佳、神谷典穂、後藤雅宏
    • Organizer
      第62回高分子討論会
    • Place of Presentation
      金沢大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 酵素応答性ペプチドハイドロゲルのネットワーク形成制御2013

    • Author(s)
      古賀未佳、若林里衣、神谷典穂、後藤雅宏
    • Organizer
      日本化学会第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Directed Aggregation and Fusion of vesicles induced by a DNA surfactant2013

    • Author(s)
      M. Goto
    • Organizer
      AIChE Annual Meeting, 2013
    • Place of Presentation
      サンフランシスコ
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Split Spy0128のバイオコンジュゲーションツールとしての利用2012

    • Author(s)
      安倍弘喜, 若林里衣, 後藤雅宏, 神谷典穂
    • Organizer
      酵素工学研究会第68回講演会
    • Related Report
      2013 Final Research Report
  • [Presentation] 酵素反応を利用した高分子-タンパク質ハイブリッドゲルの作製2012

    • Author(s)
      森山幸祐, 後藤雅宏, 神谷典穂
    • Organizer
      化学工学会第44回秋季大会
    • Related Report
      2013 Final Research Report
  • [Presentation] Lipid Modification of Proteins Catalyzed by Transglutaminase2012

    • Author(s)
      H. Abe, M. Goto, N. Kamiya
    • Organizer
      2nd International Conference on Molecular & Functional Catalysis (ICMFC-2)
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Functional protein assemblies by site-specific avidin-biotin interactions2012

    • Author(s)
      Y. Mori, M. Goto, N. Kamiya
    • Organizer
      2th Japan-China-Korea Joint Symposium on Enzyme Engineering
    • Related Report
      2013 Final Research Report
  • [Presentation] 酵素反応を活用した機能性ハイドロゲルの創製2012

    • Author(s)
      森山幸祐、後藤雅宏、神谷典穂
    • Organizer
      第23回九州地区ケミカルエンジニアリング討論会
    • Place of Presentation
      北九州
    • Related Report
      2012 Research-status Report
  • [Presentation] 基質を液体化することで構築される新たな酵素反応場2012

    • Author(s)
      中古場輝満、後藤雅宏、神谷典穂
    • Organizer
      第23回九州地区ケミカルエンジニアリング討論会
    • Place of Presentation
      北九州
    • Related Report
      2012 Research-status Report
  • [Presentation] 酵素反応を利用した高分子-タンパク質ハイブリッドゲルの作製2012

    • Author(s)
      森山幸祐、後藤雅宏、神谷典穂
    • Organizer
      化学工学会第44回秋季大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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