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nanomanipulationof antibody based on liquid-binding peptide

Research Project

Project/Area Number 24656496
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionTohoku University

Principal Investigator

UMETSU Mitsuo  東北大学, 工学(系)研究科(研究院), 准教授 (70333846)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsナノバイオ / 分子認識 / 蛋白質
Research Abstract

Recently, we identified the peptide with affinity for liquid surface. In this study, we tried to use the peptide for manipulating proteins on liquid surface and clustering peptide in water solution. Consequently, the fusion of the liquid-binding peptide to GFP resulting in the formation of GFP membrane on the water-ionic liquid interface and the fusion of the peptide to antibody fragment led to the clustering of antibody fragment with avidity effect.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (33 results)

All 2013 2012

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (20 results) (of which Invited: 6 results) Book (1 results)

  • [Journal Article] Application of 300x Enhanced Fluorescence on a Plasmonic Chip Modified with a Bispecific Antibody to a Sensitive Immunosensor2013

    • Author(s)
      Keiko Tawa, Mitsuo Umetsu, Hikaru Nakazawa, Takamitsu Hattori, Izumi Kumagai
    • Journal Title

      ACS Applied Materials & Interfaces

      Volume: 5 Issue: 17 Pages: 8628-8632

    • DOI

      10.1021/am402173y

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hybrid Nanocellulosome Design from Cellulase Modules on Nanoparticles : Synergistic Effect of Catalytically Divergent Cellulase Modules on Cellulose Degradation Activity2013

    • Author(s)
      Hikaru Nakazawa, Do-Myoung Kim, Takeshi Matsuyama, Nobuhiro Ishida, Akinori Ikeuchi, Yuri Ishigaki, Izumi Kumagai, Mitsuo Umetsu
    • Journal Title

      ACS Catalysis

      Volume: 3 Issue: 6 Pages: 1342-1348

    • DOI

      10.1021/cs400012v

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid and Sensitive Detection of Brain-Derived Neurotrophic Factor with a Plasmonic Chip2013

    • Author(s)
      Keiko Tawa, Mari Satoh, Koichi Uegaki, Tomoko Hara, Masami Kojima, Hiroyuki Aota, Yoshiki Yokota, Takahiko Nakaoki, Mitsuo Umetsu, Hikaru Nakazawa, Izumi Kumagai
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 52 Issue: 6S Pages: 06GK01-06GK01

    • DOI

      10.7567/jjap.52.06gk01

    • NAID

      210000142391

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biomass-binding peptides designed by molecular evolution for efficient degradation of cellulose in biomass by cellulase2013

    • Author(s)
      Hikaru Nakazawa, Akinori Ikeuchi, Do-Myoung Kim, Yuri Ishigaki, Hidetaka Asano, Katsunori Kouda, Izumi Kumagai, Mitsuo Umetsu
    • Journal Title

      Green Chemistry

      Volume: 15 Issue: 2 Pages: 365-369

    • DOI

      10.1039/c2gc36914a

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] In-one-pot-at-a-time ligation for high-throughput construction of a protein expression vector library2013

    • Author(s)
      Hikaru Nakazawa, Rui Todokoro, Yuri Ishigaki, Izumi Kumagai, and Mitsuo Umetsu
    • Journal Title

      Chemistry letters

      Volume: 42 Pages: 424-426

    • NAID

      10031162195

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] In-one-pot-at-a-time Ligation for High-throughput Construction of a Protein Expression Vector Library2013

    • Author(s)
      Hikaru Nakazawa, Rui Todokoro, Yuri Ishigaki, Izumi Kumagai, Mitsuo Umetsu
    • Journal Title

      Chemistry letters

      Volume: 42 Pages: 424-426

    • NAID

      10031162195

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide2012

    • Author(s)
      Takashi Matsuyama, Do-Myoung Kim, Mitsuo Umetsu, Taiji Ikawa, Mamoru Yamanishi, Nobuhiro Ishida, Izumi Kumagai, and Haruo Takahashi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 113 Issue: 2 Pages: 160-165

    • DOI

      10.1016/j.jbiosc.2011.09.016

    • NAID

      110009418662

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A high-affinity gold-binding camel antibody: Antibody engineering for one-pot functionalization of gold nanoparticles as biointerface molecules2012

    • Author(s)
      Takamitsu Hattori, Mitsuo Umetsu, Takeshi Nakanishi, Satoko Sawai, Shinsuke Kikuchi, Ryutaro Asano, and Izumi Kumagai
    • Journal Title

      Bioconjugate Chemistry

      Volume: 23(9) Issue: 9 Pages: 1934-1944

    • DOI

      10.1021/bc300316p

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nanocluster design for the construction of artificial cellulosomes2012

    • Author(s)
      Do-Myoung Kim, Hikaru Nakazawa, Mitsuo Umetsu, Takashi Matsuyama, Nobuhiro Ishida, Akinori Ikeuchi, Haruo Takahashi, Ryutaro Asano, and Izumi Kumagai
    • Journal Title

      Catalysis Science & Technology

      Volume: 2 Issue: 3 Pages: 499-503

    • DOI

      10.1039/c2cy00371f

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quantum Dot Motion on Microtubules2012

    • Author(s)
      Aurélien Sikora, Daniel Oliveira, Kyongwan Kim, Andrew L. Liao, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Wonmuk Hwang, and Winfried Teizer
    • Journal Title

      Chemistry Letters

      Volume: 41 Issue: 10 Pages: 1215-1217

    • DOI

      10.1246/cl.2012.1215

    • NAID

      10031120636

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The assembly of kinesin-based nanotransport systems2012

    • Author(s)
      D. Oliveira, D.-M. Kim, M. Umetsu, I. Kumagai, T. Adschiri, W. Teizer
    • Journal Title

      J. Appl. Phys

      Volume: 112 Issue: 12

    • DOI

      10.1063/1.4769870

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] ボトムアップな粒子アセンブリツールとしてのバイオ分子2012

    • Author(s)
      梅津 光央
    • Journal Title

      セラミックス

      Volume: 47 Pages: 616-619

    • NAID

      10031155593

    • Related Report
      2012 Research-status Report
  • [Presentation] 抗体フォールドを用いたインターフェイスデザイン : ナノ世界の糊として2013

    • Author(s)
      梅津光央
    • Organizer
      未来化学創造センターナノバイオアセンブリワークショップ
    • Place of Presentation
      福岡
    • Year and Date
      2013-11-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Smart bio-design for interface molecules between nanomaterials2013

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      日本化学会東北支部70周年記念国際会議
    • Place of Presentation
      仙台
    • Year and Date
      2013-09-28
    • Related Report
      2013 Final Research Report
  • [Presentation] ナノを意識した蛋白質スマートデザイン2013

    • Author(s)
      梅津光央
    • Organizer
      生体機能関連化学部会第28回若手フォーラム
    • Place of Presentation
      名古屋
    • Year and Date
      2013-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Smart antibody desgined from protein engineering2013

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      2013 Korean Society for Biotechnology and Bioengineering (KSBB) Spring Meeting & International Symposium
    • Place of Presentation
      Gwangju, Korea
    • Year and Date
      2013-04-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Material-binding antibody as an interface tool for manipulating nano-materials2013

    • Author(s)
      Mitsuo Umetsu, Takamitsu Hattori, Takeshi Nakanishi, and Izumi Kumagai
    • Organizer
      2013 MRS Spring Meeting
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2013-04-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Material-binding antibody as an interface tool for manipulating nano-materials2013

    • Author(s)
      Mitsuo Umetsu, Takamitsu Hattori, Takeshi Nakanishi, Izumi Kumagai
    • Organizer
      2013 Materisl Research Society Spring Meeting
    • Place of Presentation
      San Francisco (USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Smart antibody desgined from protein engineering2013

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      2013 Korean Society for Biotechnology and Bioengineering Spring Meeting & International Symposium
    • Place of Presentation
      Gwangju (Korea)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ナノを意識した蛋白質スマートデザイン2013

    • Author(s)
      梅津 光央
    • Organizer
      生体機能関連化学部会 第28回若手フォーラム
    • Place of Presentation
      名古屋大学 (名古屋市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Smart bio-design for interface molecules between nanomaterials2013

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      日本化学会東北支部70周年記念国際会議
    • Place of Presentation
      東北大学 (仙台市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 抗体フォールドを用いたインターフェイスデザイン:ナノ世界の糊として2013

    • Author(s)
      梅津 光央
    • Organizer
      未来化学創造センター ナノバイオアセンブリワークショップ
    • Place of Presentation
      福岡市産学連携交流センター (福岡)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Build up Design for Multivalent Antibodies on Multimeric Protein Platform2012

    • Author(s)
      Rui Todokoro, Mitsuo Umetsu, Hikaru Nakazawa, Ryutaro Asano, Izumi Kumagai
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Restructure of Sequential CDR Walking Approach in CAnIGET Method2012

    • Author(s)
      Yasuko Seta, Mitsuo Umetsu, Hikaru Nakazawa, Seiichi Takami, Izumi Kumagai
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-12
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] LEGO design for multivalent antibodies expressed in E.coli2012

    • Author(s)
      Rui Todokoro, Hikaru Nakazawa, Ryutaro Asano, Mitsuo Umetsu, Izumi Kumagai
    • Organizer
      Young Asian Biochemical Engineers' Community (YABEC) 2012
    • Place of Presentation
      徳島
    • Year and Date
      2012-10-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Smart Bio-Design from Protein Engineering in the Field of Nanobiotechnology2012

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      Young Asian Biochemical Engineers' Community (YABEC) 2012
    • Place of Presentation
      徳島
    • Year and Date
      2012-10-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Protein engineering for site-specific bioconjugation chemistry : Construction of multiple functional lowmolecular antibodies2012

    • Author(s)
      Mitsuo Umetsu, Asami Ueda, Takeshi Nakanishi, Kentaro Hashikami, Ryutaro Asano, Izumi Kumagai
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Protein Engineering for Nano Engineering2012

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      2012 Northeastern Asian Symposium
    • Place of Presentation
      仙台
    • Year and Date
      2012-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Smart protein design for interface molecules in nano world2012

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      2012 Northeastern Asian Symposium
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Protein engineering for site-specific bioconjugation chemistry: Construction of multiple functional lowmolecular antibodies2012

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] Smart Bio-Design from Protein Engineering in the Field of Nanobiotechnology2012

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      The 19th Symposium of Young Asian Biochemical Engineers' Community (YABEC) 2012
    • Place of Presentation
      Tokushima, Japan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] LEGO design for multivalent antibodies expressed in E.coli2012

    • Author(s)
      Rui Todokoro
    • Organizer
      The 19th Symposium of Young Asian Biochemical Engineers' Community (YABEC) 2012
    • Place of Presentation
      Tokushima, Japan
    • Related Report
      2012 Research-status Report
  • [Book] 進化分子工学2013

    • Author(s)
      梅津 光央、中澤 光、服部 峰充
    • Total Pages
      424
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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