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Application of low-shear modeled microgravity culture for screening of new microbes and it's evaluation

Research Project

Project/Area Number 24656497
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionUniversity of Tsukuba

Principal Investigator

AOYAGI Hideki  筑波大学, 生命環境系, 教授 (00251025)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords模擬微小重力培養 / 微生物 / 細菌 / スクリーニング / VBNC / ultramicrobacteria / バイオリアクター / filtration / バイオテクノロジー
Research Abstract

The effects of low-shear modeled microgravity (LSMMG) on a microbial community were investigated, and the potential application of LSMMG culture in the screening of new microorganisms was evaluated. The cell growth and sugar consumption rates, and final cell concentrations in LSMMG culture were higher than those of normal-gravity culture. Three groups of microorganisms were observed: a) the growth was promoted by LSMMG; b) the growth was suppressed by LSMMG; and c) the growth was not affected by LSMMG. The microorganisms whose growth were promoted by LSMMG showed that some had high similarity with microorganisms that have not yet been isolated. The LSMMG culture is useful for screening of new microorganisms.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (13 results)

All 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (8 results)

  • [Journal Article] 模擬微小重力培養が孔径0.2-μmのフィルターで濾過した微生物叢に及ぼす影響の解析と新規微生物スクリーニングへの応用2014

    • Author(s)
      青柳秀紀、黒田晶葉
    • Journal Title

      生物工学会誌

      Volume: 92 Pages: 72-72

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 微生物の新規培養法の開発と利用-模擬微小重力培養を活用した微生物の新規スクリーニング法の開発-2012

    • Author(s)
      青柳秀紀
    • Journal Title

      ケミカルエンジニアリング

      Volume: 57 Pages: 889-893

    • Related Report
      2013 Final Research Report
  • [Journal Article] Effects of low-shear modeled microgravity on a microbial community filtered through a 0.2-μm filter and its potential application in screening for novel microorganisms2012

    • Author(s)
      Hideki Aoyagi, Akiha Kuroda
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 114 Pages: 73-79

    • NAID

      110009479369

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of low-shear modeled microgravity on a microbial community filtered through a 0.2-μm filter and its potential application in screening for novel microorganisms.2012

    • Author(s)
      Hideki Aoyagi and Akiha Kuroda
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 114 Pages: 73-79

    • NAID

      110009479369

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 微生物の新規培養法の開発と利用ー模擬微小重力培養を活用した微生物の新規スクリーニング法の開発ー2012

    • Author(s)
      青柳秀紀
    • Journal Title

      ケミカルエンジニアリング

      Volume: 57 Pages: 889-893

    • Related Report
      2012 Research-status Report
  • [Presentation] Effect of low-shear modeled microgravity on a microbial community and its potential application2014

    • Author(s)
      Hideki Aoyagi
    • Organizer
      XIV^<th> International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Montreal, Quebec, Canada(accepted : 2014年4月20日)
    • Year and Date
      2014-07-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Effects of low-shear modeled microgravity on a microbial community and its potential application.2014

    • Author(s)
      HIdeki Aoyagi
    • Organizer
      XIVth International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Montreal, Quebec, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] カタラーゼとピルビン酸が孔径0.2μmのフィルターで濾過した環境サンプル中の微生物叢に及ぼす影響の解析と利用2013

    • Author(s)
      纐纈智也、青柳秀紀
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2013-09-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Effect of low-shear modeled microgravity on microbial community and its application in screening for novel microorganisms2013

    • Author(s)
      Tomoya Kouketsu, Hideki Aoyagi
    • Organizer
      University of the Philippines Los Banos
    • Place of Presentation
      University of the Philippines Los Banos, Philippine
    • Year and Date
      2013-03-06
    • Related Report
      2013 Final Research Report
  • [Presentation] カタラーゼとピルビン酸が孔径0.2 μmのフィルターで濾過した環境サンプル中の微生物叢に及ぼす影響の解析と利用2013

    • Author(s)
      纐纈智也、青柳秀紀
    • Organizer
      日本生物工学会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 模擬微小重力培養を活用した微生物の新規スクリーニング法の開発2012

    • Author(s)
      黒田晶葉、纐纈智也、青柳秀紀
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2012-10-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 模擬微小重力培養を活用した微生物の新規スクリーニング法の開発

    • Author(s)
      黒田晶葉、纐纈智也、青柳秀紀
    • Organizer
      第64回日本生物工学会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] Effect of low-shear modeled microgravity on microbial community and its application in screening for novel microorganisms

    • Author(s)
      Tomoya Kouketsu and Hideki Aoyagi
    • Organizer
      フィリピン大学ロスバニョス校
    • Place of Presentation
      フィリピン大学ロスバニョス校、フィリピン
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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