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Development of selective cell collection method using photo-degradable PEG-lipid

Research Project

Project/Area Number 24656498
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionThe University of Tokyo

Principal Investigator

NAGAMUNE Teruyuki  東京大学, 工学(系)研究科(研究院), 教授 (20124373)

Co-Investigator(Renkei-kenkyūsha) YAMAGUCHI Satoshi  東京大学, 先端科学技術研究センター, 講師 (80398106)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords光分解性PEG脂質 / 細胞パターニング / 一細胞アレイ / 細胞分離回収
Research Abstract

Single-cell array is a promising technology for microscopy-based high-content analyses.In this study, we constructed a versatile single cell array for both non-adherent and adherent cells by developing a photo-cleavable PEG-lipid/collagen coating material. On this material surface, we confirmed that cells behave similar to their native functions such as cell adhesion, mitosis and migration. Furthermore, we succeeded in high-throughput single cell quantitative imaging analysis on the motility of the adherent cells and that on the morphological changes of non-adherent cells using this PEG-lipid/collagen-based single-cell array, and the recovery of a target cell by photo-irradiation of this cell.This versatile single-cell array shall be invaluable tool for the research in the fields of cell engineering.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (22 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (20 results) (of which Invited: 1 results)

  • [Journal Article] Dynamic PhotoChemical Lipid Micropatterning for Manipulation of Nonadherent Mammalian Cells2014

    • Author(s)
      Yamahira, S., Takasaki, Y., Yamaguchi, S., Sumura. K., Kanamori, T. and Nagamune, T.
    • Journal Title

      Methods in Cell Biology

      Volume: 120 Pages: 131-144

    • DOI

      10.1016/b978-0-12-417136-7.00008-2

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transfer printing of transfected cell microarrays from poly(ethylene glycol)-oleyl surfaces onto biological hydrogels2013

    • Author(s)
      Han SW, Nakamura C, Miyake J, Chang SM, Adachi T
    • Journal Title

      J Nanosci Nanotechnol

      Volume: 110 Issue: 12 Pages: 3269-74

    • DOI

      10.1002/bit.25010

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 光分解性RGD-PEGを用いた細胞接着の時空間制御2014

    • Author(s)
      高橋裕美, 他3名
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋
    • Year and Date
      2014-03-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 光分解性PEG脂質修飾コラーゲン表面を用いた一細胞アレイ法の開発とその応用2014

    • Author(s)
      山平真也, 他4名
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋
    • Year and Date
      2014-03-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 光分解性PEG脂質を用いた一細胞アレイ化技術と細胞解析への応用2013

    • Author(s)
      山平真也, 他4名
    • Organizer
      化学とマイクロ・ナノシステム学会第28回研究会
    • Place of Presentation
      イーグレひめじ、姫路
    • Year and Date
      2013-12-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Light-controllable Cell Patterning using Photo-cleavable Poly(ethylene glycol)-Lipid for Photoreleasable Cell Microarray2013

    • Author(s)
      山平真也, 他4名
    • Organizer
      第13回東京大学生命科学シンポジウム
    • Place of Presentation
      東京大学、東京
    • Year and Date
      2013-06-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 光分解性PEG脂質表面上での一細胞レベルマニピュレーション技術の開発2013

    • Author(s)
      山平真也, 他4名
    • Organizer
      化学工学会第78年会
    • Place of Presentation
      大阪大学豊中キャンパス、大阪
    • Year and Date
      2013-03-17
    • Related Report
      2013 Final Research Report
  • [Presentation] PEG脂質を用いた次世代細胞マイクロアレイの開発2012

    • Author(s)
      山口哲志、長棟輝行
    • Organizer
      第29回医用高分子研究会講座
    • Place of Presentation
      産総研臨界副都心センター,東京
    • Year and Date
      2012-11-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Spatio-temporal Cell Micro-Patterning with Photo- Cleavable PEG-lipid2012

    • Author(s)
      Yamaguchi, S., 他4名
    • Organizer
      AIChE annual meeting
    • Place of Presentation
      Pittsburgh, USA
    • Year and Date
      2012-11-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Light- controllable Cell Patterning using Photo-cleavable Poly(ethylene glycol) -Lipid for Photoreleasable Cell Microarray2012

    • Author(s)
      Yamaguchi, S., 他4名
    • Organizer
      The 19th Symposium of Young Asian Biochemical Engineers' Community
    • Place of Presentation
      徳島大学,徳島
    • Year and Date
      2012-10-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Spatio-temporal cell patterning technology using a photocleavable cell anchoring reagent2012

    • Author(s)
      Yamaguchi, S., 他4名
    • Organizer
      15th International Biotechnology Symposium and Exhibition (IBS2012)
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2012-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Spatio -temporal control of cell micro-patterns on photo-cleavable PEG-lipid surfaces2012

    • Author(s)
      Yamaguchi, S., 他4名
    • Organizer
      The Second Asian Chemical Biology Conference (ACBC2012)
    • Place of Presentation
      Southern Beach Hotel & Resort,沖縄
    • Year and Date
      2012-07-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Light-controllable Cell Patterning using Photo-cleavable Poly(ethylene glycol)-Lipid for Photoreleasable Cell Microarray

    • Author(s)
      山平真也、 山口哲志、 須丸公雄、 金森敏幸、 長棟輝行
    • Organizer
      第13回東京大学生命科学シンポジウム
    • Place of Presentation
      東京大学本郷キャンパス、東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 光分解性PEG 脂質を用いた一細胞アレイ化技術と細胞解析への応用

    • Author(s)
      山平真也、 山口哲志、 須丸公雄、 金森敏幸、 長棟輝行
    • Organizer
      化学とマイクロ・ナノシステム学会第28回研究会
    • Place of Presentation
      イーグレひめじ、兵庫
    • Related Report
      2013 Annual Research Report
  • [Presentation] 光分解性PEG 脂質修飾コラーゲン表面を用いた一細胞アレイ法の開発とその応用

    • Author(s)
      山平真也、 山口哲志、 長棟輝行
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学東山キャンパス、愛知
    • Related Report
      2013 Annual Research Report
  • [Presentation] 光分解性RGD-PEGを用いた細胞接着の時空間制御

    • Author(s)
      高崎裕美、 山口哲志、 山平真也、 長棟輝行
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学東山キャンパス、愛知
    • Related Report
      2013 Annual Research Report
  • [Presentation] Spatio-temporal control of cell micro-patterns on photo-cleavable PEG-lipid surface

    • Author(s)
      S. Yamaguchi, S. Yamahira, K. Sumaru, T. Kanamori, T. Nagamune
    • Organizer
      The Second Asian Chemical Biology Conference (ACBC2012)
    • Place of Presentation
      Okinawa, Southern Beach Hotel & Resort
    • Related Report
      2012 Research-status Report
  • [Presentation] Spatio-temporal cell patterning technology using a photocleavable cell anchoring reagent

    • Author(s)
      Shinya Yamahira, Satoshi Yamaguchi, Kyoko Kikuchi, Kimio Sumaru, Toshiyuki Kanamori, Teruyuki Nagamune
    • Organizer
      15th International Biotechnology Symposium and Exhibition (IBS2012)
    • Place of Presentation
      Daegu, Korea
    • Related Report
      2012 Research-status Report
  • [Presentation] Light-controllable Cell Patterning using Photo-cleavable Poly(ethylene glycol)-Lipid for Photoreleasable Cell Microarray

    • Author(s)
      Yamahira Shinya, Yamaguchi Satoshi, Sumaru Kimio, Kanamori Toshiyuki, Nagamune Teruyuki
    • Organizer
      The 19th Symposium of Young Asian Biochemical Engineers' Community (YABEC2012)
    • Place of Presentation
      Tokushima, Tokushima University
    • Related Report
      2012 Research-status Report
  • [Presentation] Spatio-temporal Cell Micro-Patterning with Photo-Cleavable PEG-lipid

    • Author(s)
      Satoshi Yamaguchi, Shinya Yamahira, Kimio Sumaru, Toshiyuki Kanamori and Teruyuki Nagamune
    • Organizer
      2012 AIChE annual meeting
    • Place of Presentation
      Pittsburgh, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] PEG脂質を用いた次世代細胞マイクロアレイの開発

    • Author(s)
      山口哲志, 長棟輝行
    • Organizer
      第29回医用高分子研究会講座
    • Place of Presentation
      東京、産業技術総合研究所臨界副都心センター
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 光分解性PEG脂質表面上での一細胞レベルマニピュレーション技術の開発

    • Author(s)
      山平真也, 山口哲志, 須丸公雄, 金森敏幸, 長棟輝行
    • Organizer
      化学工学会第78年会
    • Place of Presentation
      大阪、大阪大学豊中キャンパス
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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