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Detection system using elecrochemical glucose sensor system and the change of nano-structure of aptamers

Research Project

Project/Area Number 24656499
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

IKEBUKURO Kazunori  東京農工大学, 工学(系)研究科(研究院), 教授 (70251494)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsアプタマー / 血管内皮成長因子 / 電気化学検出 / 疾病マーカー / ナノ構造 / グルコースデヒドロゲナーゼ / 血糖値センサー / 血管内皮細胞増殖因子 / 電気化学測定 / バイオセンシング / VEGF
Research Abstract

We develped the Gq-switch by connecting the glucose dehydrogenase (GDH)-binding aptamer and the vascular endothelial growth factor (VEGF)-binding aptamer. Gq-switch forms huge 3D network structure sicne it has a lot of G-quadruplex (Gq) structure forming parts and it can be the juction of the multiple aptamers. We observed its structural change upon its binding to VEGF and checked it can also bind to GDH. The GDH used in this study can directly transfer electron to the electrode, therefore if the distance between the GDH bound to Gq-swithch and the electrode changes, we can expect the change of electric current generated by the glucose oxidation catalyzed by the GDH.
We immoblized the GDH-Gq-switch complex onto the gold electrode and obseved the change of electric current generated from the glucose addition upon the addition of VEGF. That current change was dependent on the concentration of VEGF and therefore we were able to detect VEGF with this electrochemical detection system.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2014 Other

All Presentation (2 results) Book (2 results) Remarks (1 results)

  • [Presentation] ナノ構造体を形成するアプタマーの構造変化を利用したVEGFの電気化学的検出2014

    • Author(s)
      人木田直紀、三田千福、吉田亘、阿部公一、池袋一典
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2014-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] ナノ構造体を形成するアプタマーの構造変化を利用したVEGFの電気化学的検出2014

    • Author(s)
      八木田直紀、三田千福、吉田亘、阿部公一、池袋一典
    • Organizer
      日本化学会第94春季大会
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2013 Annual Research Report
  • [Book] リボザイムとアプタマー(化学便覧応用化学編第7版)2014

    • Author(s)
      吉田亘、阿部公一、池袋一典
    • Publisher
      丸善出版株式会社
    • Related Report
      2013 Final Research Report
  • [Book] "Electrochemical Biosensors Using Aptamers for Theranostics" in "Advances in Biochemical Engineering / Biotechnology"2014

    • Author(s)
      Koichi Abe, Wataru Yoshida and Kazunori Ikebukuro
    • Publisher
      Man Bock Gu, Springer-Verlag
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.tuat.ac.jp/~kakusan/index.html

    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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