Detection system using elecrochemical glucose sensor system and the change of nano-structure of aptamers
| Project/Area Number |
24656499
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
IKEBUKURO Kazunori 東京農工大学, 工学(系)研究科(研究院), 教授 (70251494)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | アプタマー / 血管内皮成長因子 / 電気化学検出 / 疾病マーカー / ナノ構造 / グルコースデヒドロゲナーゼ / 血糖値センサー / 血管内皮細胞増殖因子 / 電気化学測定 / バイオセンシング / VEGF |
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| Research Abstract |
We develped the Gq-switch by connecting the glucose dehydrogenase (GDH)-binding aptamer and the vascular endothelial growth factor (VEGF)-binding aptamer. Gq-switch forms huge 3D network structure sicne it has a lot of G-quadruplex (Gq) structure forming parts and it can be the juction of the multiple aptamers. We observed its structural change upon its binding to VEGF and checked it can also bind to GDH. The GDH used in this study can directly transfer electron to the electrode, therefore if the distance between the GDH bound to Gq-swithch and the electrode changes, we can expect the change of electric current generated by the glucose oxidation catalyzed by the GDH.
We immoblized the GDH-Gq-switch complex onto the gold electrode and obseved the change of electric current generated from the glucose addition upon the addition of VEGF. That current change was dependent on the concentration of VEGF and therefore we were able to detect VEGF with this electrochemical detection system.
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Report
(3 results)
Research Products
(5 results)
