Construction of a cell-analyzing biochip

• Project/Area Number: 24656500
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: MIHARA Hisakazu 東京工業大学, 生命理工学研究科, 教授 (30183966)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: バイオチップ / ペプチド / 細胞

Research Abstract

To approach the goal of high-throughput analysis of cancer cells, the study on development of biochips using peptide ligands with various cell interaction abilities was performed. A peptide library showing various cell-penetration activities was successfully constructed. The different activities were converted to the color-bar codes regarded as cell finger print patterns. The mechanism of peptide cell-penetration was also examined.

Research Products

• [Journal Article] Systematic Screening of the Cellular Uptake of Designed alpha-Helix Peptides Bioorg (2013)
  • Author(s): K. Usui, T. Kikuchi, M. Mie, E. Kobatake, H. Mihara
  • Journal Title: Med. Chem. Volume: 21 Pages: 2560-2567
  • Peer Reviewed
• [Journal Article] A Peptide Release System using a Photo-cleavable Linker in a Cell Array Format for Cell-toxicity Analysis (2013)
  • Author(s): T. Kakiyama, K. Usui, K. Tomizaki, M. Mie, E. Kobatake, H. Mihara
  • Journal Title: Polymer J. Volume: 45 Pages: 535-539
  • NAID: 10031166681
  • Peer Reviewed
• [Journal Article] A Novel Array Format for Monitoring Cellular Uptake Using a Photo-Cleavable Linker for Peptide Release (2013)
  • Author(s): K. Usui, T. Kikuchi, K. Tomizaki, T. Kakiyama, H. Mihara
  • Journal Title: Chem. Commun. Volume: 49 Pages: 6394-6396
  • Peer Reviewed
• [Journal Article] Cell Penetration and Cell-selective Drug Delivery Usingα-Helix Peptides Conjugated with Gold Nanoparticles (2013)
  • Author(s): H.J. Park, H. Tsutsumi, H. Mihara
  • Journal Title: Biomaterials Volume: 34 Pages: 4872-4879
  • Peer Reviewed
• [Journal Article] A Novel Array Format for Monitoring Cellular Uptake Using a Photo-Cleavable Linker for Peptide Release (2013)
  • Author(s): K. Usui, T. Kikuchi, K. Tomizaki, T. Kakiyama, H. Mihara
  • Journal Title: Chem. Commun. Volume: 49 Issue: 57 Pages: 6394-6396
  • DOI: 10.1039/c3cc41632a
  • Peer Reviewed
• [Journal Article] Cell Penetration and Cell-selective Drug Delivery Using α-Helix Peptides Conjugated with Gold Nanoparticles (2013)
  • Author(s): H.J. Park, H. Tsutsumi, H. Mihara
  • Journal Title: Biomaterials Volume: 34 Issue: 20 Pages: 4872-4879
  • DOI: 10.1016/j.biomaterials.2013.03.049
  • Peer Reviewed
• [Journal Article] Systematic Screening of the Cellular Uptake of Designed alpha-Helix Peptides (2013)
  • Author(s): Kenji Usui, Takuya Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
  • Journal Title: Bioorg. Med. Chem. Volume: 21 Pages: 2560-2567
  • Peer Reviewed
• [Journal Article] A Peptide Release System using a Photo-cleavable Linker in a Cell Array Format for Cell-toxicity Analysis (2013)
  • Author(s): Takashi Kakiyama, Kenji Usui, Kinya Tomizaki, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
  • Journal Title: Polymer J. Volume: 45 Issue: 5 Pages: 535-539
  • DOI: 10.1038/pj.2013.20
  • NAID: 10031166681
  • Peer Reviewed
• [Presentation] Designed Peptide Libraries for Cell Analyzing Microarrays (2013)
  • Author(s): Hisakazu Mihara, Kenji Usui, Hiroshi Tsutsumi, Kiyoshi Nokihara
  • Organizer: 6^<th> International Peptide Symposium
  • Place of Presentation: Hawaii
  • Invited
• [Presentation] Designed Peptide Libraries for Cell Analyzing Microarrays (2013)
  • Author(s): H. Mihara, K. Usui, H.Tsutsumi, K. Nokihara
  • Organizer: 6th International Peptide Symposium
  • Place of Presentation: Hawaii
  • Invited
• [Presentation] αヘリックスペプチド結合金ナノ粒子を用いた細胞導入と細胞選択的ドラッグデリバリ (2012)
  • Author(s): Park Hyejin,堤浩,三原久和
  • Organizer: 第49回ペプチド討論会
  • Place of Presentation: 鹿児島
• [Presentation] 細胞透過性ペプチド結合金ナノ粒子の細胞との相互作用評価 (2012)
  • Author(s): 西村仁孝, Hyejin Park,堤浩,三原久和
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋