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Body on a Chip: in vitro living model using human pluripotent stem cells

Research Project

Project/Area Number 24656502
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionKyoto University

Principal Investigator

KAMEI Kenichiro  京都大学, 物質ー細胞統合システム拠点, 助教 (00588262)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsマイクロ流体デバイス / ヒト多能性幹細胞 / 組織工学 / ヒトES/iPS細胞 / 幹細胞 / Body on a Chip / 3次元微小組織
Research Abstract

In this research, we will develop "Body on a Chip" by accomplishing three aims, 1) development of microfluidic platform for construction of 3D micro tissues, 2) construction of 3D micro tissues using human pluripotent stem cells (hPSCs) and 3) development of "Body on a Chip" by interconnecting multiple 3D micro tissue within a microfluidic device.
Specifically, A. Development of high throughput microfluidic device (HT-microFD); B. Establishment of multiple 3D micro tissues within a HT-microFD using hPSCs; C. Construction of of "Body on a Chip" by interconnecting multiple 3D micro tissue within a microfluidic device.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Products Report
  • Research Products

    (47 results)

All 2021 2019 2018 2017 2016 2015 2014 2013 2012 Other

All Journal Article (9 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 9 results,  Open Access: 1 results) Presentation (25 results) (of which Invited: 15 results) Remarks (4 results) Patent(Industrial Property Rights) (9 results)

  • [Journal Article] Three-dimensional cultured liver-on-a-Chip with mature hepatocyte-like hcells derived from human pluripotent stem cells2019

    • Author(s)
      Kamei, Ken-ichiro; Yoshioka, Momoko; Terada, Shiho; Tokunaga, Yumie; Chen, Yong
    • Journal Title

      Biomedical Microdevices

      Volume: 21 Issue: 3 Pages: 73-73

    • DOI

      10.1007/s10544-019-0423-8

    • Related Report
      Products Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Microfluidic-nanofiber hybrid array for screening of cellular microenvironments2017

    • Author(s)
      K. Kamei, Y. Mashimo, M. Yoshioka, Y. Tokunaga, C. Fockenberg, S. Terada, M. Nakajima, T. Shibata-Seki, L. Liu, T. Akaike, E. Kobatake, E. S. How, M. Uesugi and Y. Chen
    • Journal Title

      Small

      Volume: 印刷中 Issue: 18 Pages: 1603104-1603104

    • DOI

      10.1002/smll.201603104

    • Related Report
      Products Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Integrated heart/cancer on a chip to reproduce the side effects of anti-cancer drugs in vitro2017

    • Author(s)
      Kamei, Ken-ichiro; Kato, Yoshiki; Hirai, Yoshikazu; Ito, Shinji; Satoh, Junko; Oka, Atsuko; Tsuchiya, Toshiyuki; Chen, Yong; Tabata, Osamu
    • Journal Title

      RSC Advances

      Volume: 7 Issue: 58 Pages: 36777-36786

    • DOI

      10.1039/c7ra07716e

    • NAID

      120006331959

    • Related Report
      Products Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characterization of phenotypic and transcriptional differences in human pluripotent stem cells under two- and three-dimensional culture conditions2016

    • Author(s)
      K. Kamei, Y. Koyama, Y. Mashimo, M. Yoshioka, C. Fockenberg, M. Nakashima, R. Mosbergen, O. Korn, J. Li, C. Wells and Y. Chen
    • Journal Title

      Advanced Healthcare Materials

      Volume: 5 Issue: 22 Pages: 2951-2958

    • DOI

      10.1002/adhm.201600893

    • Related Report
      Products Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of a Body-on-a-Chip Using 3-D Microstructuring Technique2016

    • Author(s)
      Y.Kato, Y.Hirai, K.Kamei, T.Tsuchiya and O.Tabata
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 136 Issue: 6 Pages: 229-236

    • DOI

      10.1541/ieejsmas.136.229

    • NAID

      130005154785

    • ISSN
      1341-8939, 1347-5525
    • Related Report
      Products Report
    • Peer Reviewed
  • [Journal Article] Nanofibrous gelatin substrates for long-term expansion of human pluripotent stem cells2014

    • Author(s)
      Li Liu, Momoko Yoshioka, Minako Nakajima, Arata Ogasawara, Jun Liu, Kouichi Hasegawa, Sisi Li, Jianli Zou, Norio Nakatsuji, Ken-ichiro Kamei*, Yong Chen*
    • Journal Title

      Biomaterial

      Volume: in press Issue: 24 Pages: 6259-6267

    • DOI

      10.1016/j.biomaterials.2014.04.024

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Body on a Chip: Re-creation of a living system in vitro2013

    • Author(s)
      K. Kamei, Y. Hirai and O. Tabata
    • Journal Title

      IEEE, Nanotechnology Magazine

      Volume: 7 Issue: 3 Pages: 6-14

    • DOI

      10.1109/mnano.2013.2275024

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cutting-edge microfabricated biomedical tools for human pluripotent stem cell research2013

    • Author(s)
      K. Kamei
    • Journal Title

      Journal of Laboratory Automation

      Volume: 18(6) Issue: 6 Pages: 469-481

    • DOI

      10.1177/2211068213495394

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phenotypic and transcriptional modulation of human pluripotent stem cells induced by nano/microfabrication materials2013

    • Author(s)
      K. Kamei, Y. Hirai, Y. Makino, M. Yoshioka, Q. Yuan, M. Nakajima, Y. Chen, and O. Tabata
    • Journal Title

      Advanced Healthcare Materials

      Volume: 2(2) Issue: 2 Pages: 287-291

    • DOI

      10.1002/adhm.201200283

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Bio-inspired microfluidic platform to control cell functions2014

    • Author(s)
      K. Kamei, Y. Mashimo, Y. Koyama, M. Yoshioka, M. Nakajima, C. Fockenberg, Y. Chen
    • Organizer
      日本化学会第94春季年会(2014)
    • Related Report
      2013 Final Research Report
  • [Presentation] High-throughput screening platform2014

    • Author(s)
      Y. Mashimo, K. Kamei, C. Fockenberg, L. Liu, Y. Koyama and Y. Chen
    • Organizer
      日本化学会第94春季年会(2014)
    • Related Report
      2013 Final Research Report
  • [Presentation] ナノファイバーを用いたヒト多能性幹細胞培養基材の開発2014

    • Author(s)
      亀井謙一郎、劉莉、吉岡桃子、長谷川光一、中辻憲夫、陳勇
    • Organizer
      日本再生医療学会総会
    • Related Report
      2013 Final Research Report
  • [Presentation] ハイスループット・マイクロ流体デバイスを用いたヒトES/iPS細胞培養用ナノファイバー・スクリーニング2014

    • Author(s)
      眞下泰正、C. Fockenberg、小山芳江、劉莉、陳勇、亀井謙一郎
    • Organizer
      日本再生医療学会総会
    • Related Report
      2013 Final Research Report
  • [Presentation] Bio-inspired microfluidic platform to control cell functions2014

    • Author(s)
      K. Kamei, Y. Mashimo, Y. Koyama, M. Yoshioka, M. Nakajima, C. Fockenberg, Y. Chen
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] High-throughput screening platform of engineered cellular microenvironments for human pluripotent stem cells2014

    • Author(s)
      Y. Mashimo, K. Kamei, C. Fockenberg, L. Liu, Y. Koyama and Y. Chen
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ナノファイバーを用いたヒト多能性幹細胞培養基材の開発2014

    • Author(s)
      亀井 謙一郎、劉 莉、吉岡 桃子、長谷川 光一、中辻 憲夫、陳 勇
    • Organizer
      日本再生医療学会総会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] ハイスループット・マイクロ流体デバイスを用いたヒトES/iPS細胞培養用ナノファイバー・スクリーニング2014

    • Author(s)
      眞下 泰正、C. Fockenberg、小山 芳江、劉 莉、陳 勇、亀井 謙一郎
    • Organizer
      日本再生医療学会総会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Bio-inspired microfluidic platform to control cell functions2013

    • Author(s)
      K.Kamei
    • Journal Title

      The 10th MicRO Alliance Meeting 2013

    • Place of Presentation
      京都大学
    • Year and Date
      2013-11-21
    • Related Report
      Products Report
    • Invited
  • [Presentation] Engineered regulatory niche to control cellular functions2013

    • Author(s)
      K.Kamei
    • Organizer
      7th Annual Symposium on Nanobiotechnology
    • Place of Presentation
      University of Bristol, Bristol, UK
    • Year and Date
      2013-11-05
    • Related Report
      Products Report
    • Invited
  • [Presentation] 「Body on a Chip」を用いた創薬スクリーニングの開発2013

    • Author(s)
      亀井 謙一郎
    • Organizer
      テルモ科学技術振興財団 特別研究助成 贈呈式 記念講演
    • Place of Presentation
      KKRホテル東京
    • Year and Date
      2013-03-14
    • Related Report
      Products Report
    • Invited
  • [Presentation] Nano/microengineering approach to investigate and control stem cell function2013

    • Author(s)
      K.Kamei
    • Organizer
      Seminar
    • Place of Presentation
      Monash University, Clayton, Australia
    • Year and Date
      2013-02-14
    • Related Report
      Products Report
    • Invited
  • [Presentation] Gelatin nanofibrous scaffold to maintain human ES/iPS cells for long-term period2013

    • Author(s)
      K.Kamei
    • Organizer
      Joint SCA and iCeMS Symposium
    • Place of Presentation
      University of Melbourne, Melbourne, Australia
    • Year and Date
      2013-02-13
    • Related Report
      Products Report
    • Invited
  • [Presentation] Gelatin nanofibrous scaffold enables maintaining human ES/iPS cells for long-term period2013

    • Author(s)
      K.Kamei
    • Organizer
      The 1st Bristol-Kyoto Symposium 2013
    • Place of Presentation
      University of Bristol, Bristol, UK
    • Year and Date
      2013-01-10
    • Related Report
      Products Report
    • Invited
  • [Presentation] Nanofiber scaffolds to maintain human pluripotent stem cells under feeder-free conditions2013

    • Author(s)
      K.Kamei
    • Organizer
      Mini-symposium of WPI-iCeMS and Edinburgh University
    • Place of Presentation
      University of Edinburgh, Edinburgh, UK
    • Year and Date
      2013-01-08
    • Related Report
      Products Report
    • Invited
  • [Presentation] Gelatin nanofibrous scaffold enables maintaining human ES/iPS cells for long-term period2013

    • Author(s)
      K. Kamei
    • Organizer
      The 1st Bristol-Kyoto Symposium 2013
    • Place of Presentation
      Bristol, UK
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Gelatin nanofibrous scaffold enables expanding human pluripotent stem cells for long-term period2012

    • Author(s)
      K. Kamei
    • Organizer
      6th Nanobiotechnology Symposium
    • Place of Presentation
      Kyoto University, Kyoto, Japan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Artificial regulatory microenvironments (niche) for controlling cell function2012

    • Author(s)
      K. Kamei
    • Organizer
      IEEE Nanotechnology Material and Devices Conference (IEEE-NMDC)
    • Place of Presentation
      Hawaii, USA
    • Related Report
      2012 Research-status Report Products Report
    • Invited
  • [Presentation] Phenotypic modulation of pluripotent stem cells (PSCs) induced by microfabrication materials2012

    • Author(s)
      K. Kamei, Y. Hirai, Y. Makino, M. Yoshioka, M. Nakajima, Q. Yuan, Y. Chen and O. Tabata
    • Organizer
      The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)
    • Place of Presentation
      沖縄
    • Related Report
      2012 Research-status Report
  • [Presentation] Phenotypic modulation of pluripotent stem cells by microfabrication materials2012

    • Author(s)
      K. Kamei, Y. Hirai, Y. Makino, M. Yoshioka, M. Nakajima, Q. Yuan, Y. Chen and O. Tabata
    • Organizer
      10th International Society of Stem Cell Research (ISSCR) Annual Meeting,
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Body on a Chip:人体の再構成と創薬への応用

    • Author(s)
      亀井 謙一郎
    • Organizer
      電子情報技術部会、MEMS分科会講演会
    • Place of Presentation
      公益社団法人 新化学技術推進協会
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Nano/microengineering approach to investigate and control stem cell function

    • Author(s)
      K. Kamei
    • Organizer
      Monash University
    • Place of Presentation
      Monash University, Clayton, Australia
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Gelatin nanofibrous scaffold to maintain human ES/iPS cells for long-term period

    • Author(s)
      K. Kamei
    • Organizer
      Joint SCA and iCeMS Symposium
    • Place of Presentation
      University of Melbourne, Melbourne, Australia
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Nanofiber scaffolds to maintain human pluripotent stem cells under feeder-free conditions

    • Author(s)
      K. Kamei
    • Organizer
      Mini-symposium of WPI-iCeMS and Edinburg University
    • Place of Presentation
      Edinburg, UK
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Phenotypic and transcriptional modulation of human pluripotent stem cells induced by nano/microfabrication materials

    • Author(s)
      K. Kamei
    • Organizer
      MicRO Alliance Meeting 2012
    • Place of Presentation
      University of Freiburg, Freiburg, Germany
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks]

    • URL

      http://www.chen.icems.kyoto-u.ac.jp/

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://ken1kamei.wordpress.com/

    • Related Report
      2013 Final Research Report
  • [Remarks] Ken-ichiro Kamei @ iCeMS, Kyoto University

    • URL

      http://ken1kamei.wordpress.com

    • Related Report
      2012 Research-status Report
  • [Remarks] Chen Group, iCeMS, Kyoto Unversity

    • URL

      http://www.chen.icems.kyoto-u.ac.jp

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] マイクロ流体デバイス及び細胞の微小3次元培養法2021

    • Inventor(s)
      亀井謙一郎、陳勇
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2021
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] マイクロ流体デバイス及び細胞の微小3次元培養法2019

    • Inventor(s)
      亀井 謙一郎、陳 勇
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2019
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] 細胞培養足場基材、マイクロ流体デバイス及びそれを用いたハイスループットナノファイバースクリーニング方法2018

    • Inventor(s)
      亀井 謙一郎、眞下 泰正、劉 莉、陳 勇
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2018
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] マイクロ流体デバイス及び細胞の微小3次元培養法2016

    • Inventor(s)
      亀井 謙一郎, 陳 勇
    • Industrial Property Rights Holder
      京都大学
    • Filing Date
      2016-07-14
    • Description
      国外に移行中
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] 内胚葉系細胞の製造方法、肝臓細胞の製造方法、内胚葉系細胞の誘導促進剤、肝臓細胞の誘導促進キット、およびマイクロ流体デバイス2015

    • Inventor(s)
      亀井 謙一郎, 本田 桃子
    • Industrial Property Rights Holder
      京都大学
    • Filing Date
      2015-12-22
    • Description
      提出日:2014年12月24日 国外に移行中
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] マイクロ流体デバイスを用いたヒト多能性幹細胞の微小3次元培養法2014

    • Inventor(s)
      亀井 謙一郎、陳 勇
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-02-25
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] マイクロ流体デバイスを用いたヒト多能性幹細胞の微小3次元培養法2014

    • Inventor(s)
      亀井 謙一郎、陳 勇
    • Industrial Property Rights Holder
      亀井 謙一郎、陳 勇
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-02-25
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞培養足場基材、マイクロ流体デバイス及びそれを用いたハイスループットナノファイバースクリーニング方法2013

    • Inventor(s)
      亀井 謙一郎、眞下 泰正、劉 莉、陳 勇
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-12-20
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 細胞培養足場基材、マイクロ流体デバイス及びそれを用いたハイスループットナノファイバースクリーニング方法2013

    • Inventor(s)
      亀井 謙一郎、眞下 泰正、劉 莉、陳 勇
    • Industrial Property Rights Holder
      亀井 謙一郎、眞下 泰正、劉 莉、陳 勇
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-12-20
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2023-03-30  

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