Chiral lipidomics by using supercritical fluid chromatography/mass spectrometry
| Project/Area Number |
24656503
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
BAMBA Takeshi 大阪大学, 工学(系)研究科(研究院), 教授 (10432444)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
FUKUSAKI Eiichiro 大阪大学, 大学院工学研究科, 教授 (40273594)
YOSHIDA Masaru 神戸大学, 大学院医学研究科, 准教授 (00419475)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | enantiomer / lipidomics / supercritical fluid / mass spectrometry / chiral analysis / 超臨界流体 / 超臨界流体クロマトグラフィー / SFC/MS / 脂質 / 光学異性体 / キラルリピドミクス / 質量分析 |
|---|
| Outline of Final Research Achievements |
We applied supercritical fluid chromatography (SFC) to the enantiomeric separation of lipids and tried to construct the chiral lipidomics system. We successfully separated triacylglycerol (TAG) enantiomeric isomers with connected chiral columns. Additionally, SFC also allows us to separate TAG positional isomers. Furthermore, we performed the analyses of biologicalsamples by using the constructed chiral lipidomics system. We achieved the enantiomeric separation of TAG enantiomeric isomers and positional isomers in edible oils. Additionally, we performed the chiral lipidomics in diabetes and myocardial infarction samples and obtained specific profiles.
|
Report
(4 results)
Research Products
(59 results)
-
-
-
-
-
-
-
[Journal Article] Development of a lipid profiling system using reverse-phase liquid chromatography coupled to high-resolution mass spectrometry with rapid polarity switching and an automated lipid identification software2013
Author(s)
Yamada, T., Uchikata, T., Sakamoto, S., Yokoi, Y., Fukusaki, E., Bamba, T
-
Journal Title
J. Chromatogra. A
Volume: 1292
Pages: 211-218
DOI
Related Report
Peer Reviewed
-
[Journal Article] Data Assessment and Metabolite Identification Tool for Large-scale MRM-based Widely Targeted Metabolomics2013
Author(s)
Tsugawa, H., Arita, M., Kanazawa, M., Ogiwara, A., Bamba, T., Fukusaki, E
-
Journal Title
Anal. Chem
Volume: 85(10)
Issue: 10
Pages: 5191-5199
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 実践脂質メタボロミクス2014
Author(s)
馬場健史
Organizer
第66回日本生物工学会大会シンポジウム
Place of Presentation
札幌コンベンションセンター（北海道，札幌市）
Year and Date
2014-09-11
Related Report
Invited
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
