Analysis and application of new translation system
| Project/Area Number |
24656511
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
ABE Hiroshi 独立行政法人理化学研究所, 伊藤ナノ医工学研究室, 専任研究員 (80415067)
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| Project Period (FY) |
2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | mRNA / 翻訳 / タンパク質合成 / 環状RNA / 原核生物 / リボソーム / 終わりのない回転式翻訳 / RNA / タンパク質 / 環状 |
|---|
| Research Abstract |
Small circular RNA molecules containing an infinite open reading frame were synthesized and tested in an E. coli cell-free translation system. A circular RNA 126 nucleotides in length was found to produce more product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA in this continuous peptide synthesis.
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Report
(2 results)
Research Products
(3 results)
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[Journal Article] Rolling circle amplification in prokaryotic translation system using small circular RNA.2013
Author(s)
Abe, N., Hiroshima, M., Maruyama, H., Nakashima, Y., Nakano, Y., Matsuda, A., Sako, Y., Ito, Y., and Abe, H.
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Journal Title
Angew. Chem. Int. Ed.
Volume: 52
Issue: 27
Pages: 7004-7008
DOI
Related Report
Peer Reviewed
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