Identification and functional characterization of anandamide receptors in Caenorhabditis elegans as a model organism.

• Project/Area Number: 24657001
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Nagoya University
• Principal Investigator: MATSUMOTO Kunihiro 名古屋大学, 理学(系)研究科(研究院), 教授 (70116375)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 遺伝学 / 遺伝子 / シグナル伝達 / 神経科学 / 生理活性

Research Abstract

Anandamide, a member of endocannabinoids, is known to be involved in many
physiological processes. CB1, CB2 and TRPV act as anandamide receptors in mammals. In addition, it has been suggested that mammals have an unidentified receptor CBx for anandamide. To identify new anandamide receptors, we performed a functional screening by using a nematode Caenorhabditis elegans as a model organism. As a result, we successfully identified new nematode seven-transmembrane receptors, designated as SFAH-1 and SFAH-2. SFAH-1 shows a weak similarity to CB1 and CB2 receptors, whereas SFAH-2 shows a similarity to a certain kind of vertebrate peptide receptor.

Research Products

• [Journal Article] Endocannabinoid-Goαsignalling inhibits axon regeneration in Caenorhabditis elegans by antagonizing the Gqα-PKC-JNK signaling (2012)
  • Author(s): Pastuhov, S. Iv., Fujiki, K., Nix, P., Kanao, S., Bastiani, M., Matsumoto, K., and Hisamoto, N.
  • Journal Title: Nature Commun Volume: 3
  • Peer Reviewed
• [Journal Article] Endocannabinoid-Goα signalling inhibits axon regeneration in Caenorhabditis elegans by antagonizing Gqα-PKC-JNK signalling. (2012)
  • Author(s): Pastuhov et al.
  • Journal Title: Nature Communications Volume: 3 Issue: 1 Pages: 1136-1136
  • DOI: 10.1038/ncomms2136
  • Peer Reviewed
• [Presentation] Endocannabinoid AEA as injury signal in axon regeneration (2013)
  • Author(s): パストゥホフストラヒル、久本直毅、松本邦弘
  • Organizer: 第19回国際線虫学会(2013 International worm meeting)
  • Place of Presentation: アメリカカリフォルニア大学ロサンゼルス校
• [Presentation] Endocannabinoid AEA as injury signal in axon regeneration (2013)
  • Author(s): パストゥホフストラヒル、久本直毅、松本邦弘
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Endocannabinoid AEA as injury signal in axon regeneration (2013)
  • Author(s): Strahil Iv Pastuhov, Naoki Hisamoto, Kunihiro Matsumoto.
  • Organizer: 第19回国際線虫学会 （2013 International worm meeting）
  • Place of Presentation: カリフォルニア大学ロサンゼルス校（UCLA, CA, USA）
• [Presentation] Endocannabinoid regulation of JNK MAP kinase in axon regeneration (2012)
  • Author(s): パストゥホフストラヒル、藤木恒太、ニックスパオラ、金尾朱夏、バスティア二マイケル、久本直毅、松本邦弘
  • Organizer: 5th East Asia Worm Meeting
  • Place of Presentation: 台湾Chientan Youth Activity Center
• [Presentation] Regulation of Axon Regeneration by Endocannabinoids (2012)
  • Author(s): パストゥホフストラヒル、藤木恒太、ニックスパオラ、金尾朱夏、バスティア二マイケル、久本直毅、松本邦弘
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Endocannabinoid regulation of JNK MAP kinase in axon regeneration (2012)
  • Author(s): Pastuhov, S. Iv.
  • Organizer: 5th East Asia Worm Meeting
  • Place of Presentation: Chientan (Jiantan) Youth Activity Center、台北（タイペイ、台湾)
• [Remarks]
  • URL: http://bunshi3.bio.nagoya-u.ac.jp/~bunshi6/matsumoto_japanese/index.html