| Project/Area Number |
24657011
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Ecology/Environment
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MAKINO Takashi 東北大学, 大学院生命科学研究科, 助教 (20443442)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 分子生態 / 疾病生態学 / 保全生態学 / 抗菌ペプチド / 遺伝子コピー数変異 / 遺伝子コピー数 / ヤマアカガエル |
|---|
| Research Abstract |
Our previous study indicated that anti peptide, Temporin-1 in Rana ornativentirs shows copy-number variations. In order to determine DNA sequences in the regions including temporin-1, we created fosmid library and chose the clone libraries including temporin-1. Then we tried to determine these DNA sequences using the next generation sequencer (Ion PGM). The results showed that among about 40kbp DNA region, those in 33kbp were non-coding regions. The rest of the sequences contained at least three copies of temporin-1, including temporin-1Oj and temproin-1OC. This suggests that these copies of temporin might be tenderly repeated. At the present study, we could not determine complete sequences of these regions.
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