| Project/Area Number |
24657020
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Ecology/Environment
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| Research Institution | Ryukoku University |
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Principal Investigator |
KONDOH Michio 龍谷大学, 理工学部, 准教授 (30388160)
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| Co-Investigator(Kenkyū-buntansha) |
MINAMOTO Toshihumi 総合地球環境学研究所, 研究部, プロジェクト上級研究員 (50450656)
MARUYAMA Atsushi 龍谷大学, 理工学部, 講師 (70368033)
YAMANAKA Hiroki 龍谷大学, 理工学部, 講師 (60455227)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 環境DNA / 魚類群集 / DNAバーコーディング / 環境モニタリング / 琵琶湖水系 / 群集ネットワーク |
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| Research Abstract |
We have developed the molecular and theoretical techniques to establish eDNA-based fish community monitoring. The major achievements are as follows: (i) A molecular biological methodology established to detect and quantify eDNA of seven fish species of the Lake Biwa-Yodo River System; (ii) DNA release and decomposition rates of fish individual, the basic parameters, and their condition dependence experimentally determined for particular species; (iii) Field and laboratory experiments conducted to test the methods developed for eDNA-based monitoring of bluegill and Japanese giant salamander; (iv) Novel methodologies established to capture and concentrate eDNA in water samples; (v) Multispecies detection technique based on multiplex PCR studied; (vi) Novel theoretical hypotheses obtained as to community network structure and its community dynamics consequences.
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