Investigation of Daphnia as a genetic model organism

• Project/Area Number: 24657022
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Ecology/Environment
• Research Institution: Okazaki Research Facilities, National Institutes of Natural Sciences
• Principal Investigator: IGUCHI Taisen 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡﨑統合バイオサイエンスセンター, 教授 (90128588)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ミジンコ / 逆遺伝学 / エコゲノミクス / 甲殻類 / 性決定 / 生殖細胞 / 環境応答

Research Abstract

The ubiquitous, freshwater microcrustacean Daphnia has been analyzed as a model animal for monitoring ecosystem integrity. Daphnia are capable of either clonal or sexual reproduction, making them ideally suited for genetic research, but the establishment of in vitro development of the embryos and gene modification techniques has not been established. We investigate a condition for natural male production and found the Daphnia strain whose sex is solely regulated by day length. In addition, we established an optimal condition for microinjections and the following in vitro development of the embryos. The current research is preliminary but our findings provide useful knowledge for further study for genetic study for Daphnids.

Research Products

• [Journal Article] Establishment of a short-term in vivo screening method for detecting chemicals having juvenile hormone activity using adult Daphnia magna (2014)
  • Author(s): R. Abe, H. Watanabe, M. Yamamuro, T. Iguchi and N. Tatarazako
  • Journal Title: J. Appl. Toxicol Volume: 印刷中 Issue: 1 Pages: 75-82
  • DOI: 10.1002/jat.2989
  • Peer Reviewed / Open Access
• [Journal Article] Molecular impact of juvenile hormone agonists on neonatal Daphnia magna. (2014)
  • Author(s): Toyota, K., Kato, Y., Miyakawa, H., Yatsu, R., Mizutani, T., Ogino, Y., Miyagawa, S., Watanabe, H., Nishide, H., Uchiyama, I., Tatarazako, N. and Iguchi, T.
  • Journal Title: Journal of Applied Toxicology Volume: 34 Issue: 5 Pages: 537-544
  • DOI: 10.1002/jat.2922
  • Peer Reviewed
• [Journal Article] Development of a microinjection system for RNA interference in the water flea Daphnia pulex (2013)
  • Author(s): Chizue Hiruta, Kenji Toyota, Hitoshi Miyakawa, Yukiko Ogino, Shinichi Miyagawa, Norihisa Tatarazako, Joseph R Shaw, Taisen Iguchi
  • Journal Title: BMC Biotechnology Volume: 13(96) Issue: 1 Pages: 96-96
  • DOI: 10.1186/1472-6750-13-96
  • Peer Reviewed
• [Journal Article] The mutation in methoprene-tolerant alters juvenile hormone use by insects and crustaceans (2013)
  • Author(s): Hitoshi Miyakawa, Kenji Toyota, Ikumi Hirakawa, Yukiko Ogino, Shinichi Miyagawa, Shigeto Oda, Norihisa Tatarazako, Toru Miura, John K. Colbourne, Taisen Iguchi
  • Journal Title: Nature Communications Volume: ncomms2868 Issue: 1 Pages: 1856-1856
  • DOI: 10.1038/ncomms2868
  • Peer Reviewed
• [Journal Article] Genomic expression responses toward bisphenol-A toxicity in Daphnia magna in terms of reproductive activity (2013)
  • Author(s): Jeong SW, Lee SM, Yum SS, Iguchi T, Seo YR
  • Journal Title: Mol. Cell. Toxicol Volume: 9 Pages: 149-158
  • URL: http://link.springer.com/article/10.1007/s13273-013-0019-y
  • Peer Reviewed
• [Journal Article] Science and policy on endocrine disrupters must not be mixed : a reply to a"common sense"intervention by toxicology journal editors (2013)
  • Author(s): Bergman Å, Andersson AM, Becher G, van den Berg M, Blumberg B, Bjerregaard P, Bornehag CG, Bornman R, Brandt I, Brian JV, Casey SC, Fowler PA, Frouin H, Giudice LC, Iguchi T, Hass U, Jobling S, Juu A, Kidd KA, Kortenkamp A, Lind M, Martin OV, Muir D, Ochieng R, Olea N, Norrgren L, Ropstad E, Ross PS, Rudén C, Scheringer M, Skakkebaek NE, Söder O, Sonnenschein C, Soto A, Swan S, Toppari J, Tyler CR, Vandenberg LN, Vinggaard AM, Wiberg K., Zoeller RT.
  • Journal Title: Environ. Health Volume: 12 Issue: 1 Pages: 69-69
  • DOI: 10.1186/1476-069x-12-69
  • Peer Reviewed
• [Journal Article] The impact of endocrine disruption : A consensus statement on the state of the science (2013)
  • Author(s): Bergman Å, Heindel JJ, Kidd KA, Jobling S, Zoeller RT, Becher G, Bjerregaard P, Bornman R, Brandt I, Brian JV, Kortenkamp A, Muir D, Ochieng R, Skakkebaek NE, Iguchi T, Toppari J
  • Journal Title: Environ. Health Perspect. Volume: 121 Issue: 4
  • DOI: 10.1289/ehp.1205448
  • Peer Reviewed
• [Journal Article] Molecular cloning of doublesex genes of four cladocera (water flea) species (2013)
  • Author(s): Kenji Toyota, Yasuhiko Kato, Masaru Sato, Naomi Sugiura, Shinichi Miyagawa, Hitoshi Miyakawa, Hajime Watanabe, Shigeto Oda, Yukiko Ogino, Chizue Hiruta, Takeshi Mizutani, Norihisa Tatarazako, Susanne Paland, Craig Jackson, John K. Colbourne, Taisen Iguchi
  • Journal Title: BMC Genomics Volume: 14:239 Issue: 1 Pages: 239-239
  • DOI: 10.1186/1471-2164-14-239
  • Peer Reviewed
• [Journal Article] Sequencing three crocodilian genomes to illuminate the evolution of archosaurs and amniotes (2012)
  • Author(s): St John JA et al.
  • Journal Title: Genome Biol Volume: 13 Issue: 1 Pages: 415-415
  • DOI: 10.1186/gb-2012-13-1-415
  • Peer Reviewed
• [Presentation] Approach to establishing tools for gene functional analysis and cryopreservation in Daphnia pulex (2014)
  • Author(s): Hiruta C, Toyota K, Miyakawa H, Miyagawa S, Ogino Y, Tatarazako N, Shaw JR, Tanaka D, Iguchi T
  • Organizer: Daphnia Genomics Consortium 2014
  • Place of Presentation: University of Birmingham, イギリス
• [Presentation] Abortive meiosis found in the oogenesis of parthenogenetic Daphnia pulex (2014)
  • Author(s): Hiruta C, Tochinai S, Iguchi T
  • Organizer: Daphnia Genomics Consortium 2014 meeting
  • Place of Presentation: University of Birmingham, イギリス
• [Presentation] Cloning and their expression pattern of doublesex genes of cladocera (2014)
  • Author(s): Miyagawa, S., Toyota, K., Miyakawa, H., Hiruta, C., Kato, Y., Watanabe, H., Tatarazako, N., Paland, S., Jackson, C., Colbourne, J.K. and Iguchi, T.
  • Organizer: EMBO Conference; Daphnia Genomics Consortium 2014
  • Place of Presentation: バーミンガム大学(バーミンガム, イギリス)
• [Presentation] Analysis of juvenile hormone as a sex-determining hormone in Daphnia pulex. EMBO Conference; Daphnia Genomics (2014)
  • Author(s): Toyota, K., Miyakawa, H., Hiruta, C., Sumiya, E., Ogino, Y., Miyagawa, S. and Iguchi, T
  • Organizer: EMBO Conference; Daphnia Genomics Consortium 2014
  • Place of Presentation: バーミンガム大学(バーミンガム, イギリス)
• [Presentation] Approach to establishing tools for gene functional analysis and cryopreservation in Daphnia pulex. (2014)
  • Author(s): Hiruta, C., Toyota, K., Miyakawa, H., Miyagawa, S., Ogino, Y., Tatarazako, N., Shaw, J.R., Tanaka, D., and Iguchi, T.
  • Organizer: EMBO Conference; Daphnia Genomics Consortium 2014
  • Place of Presentation: バーミンガム大学(バーミンガム, イギリス)
• [Presentation] bortive meiosis found in the oogenesis of parthenogenetic Daphnia pulex. Daphnia (2014)
  • Author(s): Hiruta, C., Tochinai, S. and Iguchi, T.
  • Organizer: EMBO Conference; Daphnia Genomics Consortium 2014
  • Place of Presentation: バーミンガム大学(バーミンガム, イギリス)
• [Presentation] Ecdysteroids are involved in regulatory mechanismof a reproductive cycle in D. magna. (2014)
  • Author(s): Sumiya, E., Ogino, Y., Miyakawa, H., Hiruta, C., Toyota, K., Miyagawa, S. and Iguchi, T.
  • Organizer: EMBO Conference; Daphnia Genomics Consortium 2014
  • Place of Presentation: バーミンガム大学(バーミンガム, イギリス)
• [Presentation] nalysis of juvenile hormone as a sex-determining hormone in Daphnia pulex (2014)
  • Author(s): Miyakawa, H., Toyota, K., Hirakawa, I, Ogino, Y., Miyagawa, S., Oda, S., Tatarazako, N., Miura, T., Colbourne JK., and Iguchi, T.
  • Organizer: EMBO Conference; Daphnia Genomics Consortium 2014
  • Place of Presentation: バーミンガム大学(バーミンガム, イギリス)
• [Presentation] A single amino acid substitution in Methoprene-tolerant(Met) alters juvenile hormone use by insects and Crustaceans (2013)
  • Author(s): Miyakawa H, Toyota K, Hirakawa I, Ogino Y, Miyagawa S, Oda S, Tatarazako N, Miura T, Colbourne JK, Iguchi T
  • Organizer: Insect Hormones International Workshop 2013
  • Place of Presentation: University of Minnesota,アメリカ
• [Presentation] Environmental sex determination of water flea, Daphnia magna (2013)
  • Author(s): Iguchi T, Toyota K., Miyakawa H, Hiruta C, Ogino Y, Miyagawa S, Tatarazako N
  • Organizer: 17th International Congress of Comparative Endocrinology (ICCE 2013)
  • Place of Presentation: Facultat de Biologia at the Universitat de Barcelona, スペイン
• [Presentation] Development of an RNA interference method using microinjection in the water flea Daphnia pulex (2013)
  • Author(s): Hiruta C, Toyota K, Miyakawa H, Iguchi T
  • Organizer: 46th Ann. Meet. Jap. Soc. Devel. Biol.
  • Place of Presentation: 島根県松江市
• [Presentation] Aquatic environments and endocrine activity : The regulatory approach in Japan (2013)
  • Author(s): Iguchi T
  • Organizer: SETAC Europe 23rd Ann. Meet.
  • Place of Presentation: Glasgow, イギリス
• [Presentation] ミジンコにおける内分泌かく乱作用メカニズムの解析 (2013)
  • Author(s): 井口泰泉
  • Organizer: 環境ホルモン学会第16回研究発表会
  • Place of Presentation: 東京大学 （東京都文京区）
  • Invited
• [Presentation] オミジンコ仔虫における幼若ホルモン応答遺伝子の網羅的解析 (2013)
  • Author(s): 豊田賢治、阿部良子、渡部春奈、岡智宏、宮川一志、宮川信一、荻野由紀子、鑪迫典久、井口泰泉
  • Organizer: 環境ホルモン学会第16回研究発表会
  • Place of Presentation: 東京大学 （東京都文京区）
• [Presentation] Ecdysteroid in higher titer inhibits molting and reproduction cycle in Daphnia magna. (2013)
  • Author(s): Sumiya, E., Ogino, Y., Miyakawa, H., Hiruta, C., Toyota, K., Miyagawa, S. and Iguchi, T.
  • Organizer: 環境ホルモン学会第16回研究発表会
  • Place of Presentation: 東京大学 （東京都文京区）
• [Presentation] ミジンコDaphnia pulexにおける6種類の幼若ホルモン様物質の暴露による幼若ホルモン応答遺伝子の網羅的探索. (2013)
  • Author(s): 宮川一志、井口泰泉
  • Organizer: 環境ホルモン学会第16回研究発表会
  • Place of Presentation: 東京大学 （東京都文京区）
• [Presentation] ミジンコの性決定ホルモンとしての幼若ホルモンの新規機能の解明 (2013)
  • Author(s): 豊田賢治, 宮川一志, 蛭田千鶴江, 角谷絵里, 荻野由紀子, 宮川信一, 井口泰泉
  • Organizer: 第38回日本比較内分泌学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）
• [Presentation] オオミジンコ脱皮ホルモンによる脱皮と生殖周期の制御. (2013)
  • Author(s): 谷絵里、宮川一志、荻野由紀子、蛭田千鶴江、豊田賢治、宮川信一、井口泰泉
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山大学（岡山県岡山市）
• [Presentation] Development of an RNA interference method using microinjection in the water flea Daphnia pulex, (2013)
  • Author(s): Hiruta, C., Toyota, K., Miyakawa, H. and Iguchi, T.
  • Organizer: 日本発生生物学会第46回大会
  • Place of Presentation: くにびきメッセ（島根県松江市）
• [Presentation] Temperature-dependent sex determination and sex differentiation: alligators, fish and daphnids (2012)
  • Author(s): 井口泰泉
  • Organizer: SETAC Asia Pacific
  • Place of Presentation: ホテル日航（ 熊本県熊本市）
  • Invited
• [Presentation] オオミジンコ脱皮ホルモン合成経路遺伝子の発現変動 (2012)
  • Author(s): 角谷絵里ら
  • Organizer: 第83回日本動物学会
  • Place of Presentation: 東京大学山上会館（ 東京都文京区）
• [Presentation] ミジンコ類のMetおよびSRCを用いたin vitroにおける幼若ホルモン作用評価系の開発 (2012)
  • Author(s): 宮川一志ら
  • Organizer: 環境ホルモン学会第15回研究発表会
  • Place of Presentation: 東京大学山上会館（ 東京都文京区）
• [Presentation] Analysis of sex determining genes involved in male induction in Cladocerans treated by juvenile hormone agonist (2012)
  • Author(s): Toyota, K. et al.
  • Organizer: 環境ホルモン学会第15回研究発表会
  • Place of Presentation: 東京大学山上会館（ 東京都文京区）
• [Presentation] オオミジンコ脱皮ホルモン合成経路遺伝子の脱皮周期における時空間的発現解析 (2012)
  • Author(s): 角谷絵里ら
  • Organizer: 環境ホルモン学会第15回研究発表会
  • Place of Presentation: 東京大学山上会館（ 東京都文京区）
• [Remarks]
  • URL: http://www.nibb.ac.jp/%7Ebioenv1/index-j.html
• [Remarks] Iguchi Lab.
  • URL: http://www.nibb.ac.jp/%7Ebioenv1/index-j.html
• [Remarks] Iguchi Lab on Web
  • URL: http://www.nibb.ac.jp/%7Ebioenv1/index-j.html