Proteomic identification of dephosphorylated proteins by phosphatase protein

• Project/Area Number: 24657033
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: FUKAO YOICHIRO 奈良先端科学技術大学院大学, バイオサイエンス研究科, 特任准教授 (80432590)
• Co-Investigator(Renkei-kenkyūsha): ISHIZAKI Kimitsune 神戸大学, 理学研究科, 准教授 (00452293) ; FUJIWARA Masayuki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 特任助教 (70403350)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: プロテインホスファターゼ / 脱リン酸化 / リン酸化ペプチド精製 / HPLC / ゼニゴケ / イネ培養細胞 / PP2C / ホスファターゼ / リン酸化 / ペプチド / タンパク質 / プロテインフォスファターゼ / リン酸化ペプチド

Research Abstract

Protein phosphorylation and dephosphorylation by protein kinases and phosphatases regulate many biological events in plants. To identify as many phosphoproteins as possible, several purification methods of phosphopeptides have been established. Here, we used two kind of phosphopeptides purification columns (PhosTio and PolyMac) and each method allowed identifying 771 and 641 phosphopeptides in rice cultured cells. Also, 913 and 802 phosphopeptides were identified in rice cultured cells treated with rice blast fungus. These results indicate that phosphorylation of several proteins are induced responded to pathogen attack and may have a role in disease resistances.

Research Products

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  • URL: http://bsw3.naist.jp/plantglobal/index.shtml