Development of live cell imaging techniques for cell wall polymers

• Project/Area Number: 24657034
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: HASHIMOTO Takashi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (80180826)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: セルロース / 可視化 / 蛍光標識 / 植物細胞 / 植物

Research Abstract

Fluorescent probes that track and monitor dynamic status of biopolymers, such as cellulose microfibrils, in living plant cells are essential for next-generation dynamic studies of plant cell wall. In this study, we fused in tandem a signal peptide from cell wall protein (expansin), a crystal cellulose-binding module from cellulose degrading enzyme (cellobiohydrolase from Trichoderma reesei), a membrane-spanning domain of plasma membrane receptor (BRI1), and green fluorescent protein (GFP), and transiently expressed this fusion protein in onion epidermal cells. GFP fluorescence was detected in cortical regions as dotts but not in linear filaments. Detailed observation using transgenic plants is necessary.

Research Products

• [Remarks]
  • URL: http://bsw3.naist.jp/hashimoto/