Establishment for usage of plant parasitic nematode

• Project/Area Number: 24657035
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kumamoto University
• Principal Investigator: SAWA Shinichiro 熊本大学, 自然科学研究科, 教授 (00315748)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: nematode / arabidopsis / 挑戦的萌芽 / サツマイモネコブセンチュウ / 感染

Research Abstract

In order to establish the plant parasitic nematode as a model organism for the analysis of plant-nematode interaction, we have tried to culture the nematode, to sterilize the nematode, and to perform whole mount in situ hybridization test, to identify effector proteins by using proteome technique. Further, by using effector protein candidates, we performed Y2H screening, and identified some effector protein binding proteins.

Research Products

• [Journal Article] Light-dependent green gall formation induced by Meloidogyne incognita (2014)
  • Author(s): Nishiyama, H., Nakagami, S., Todaka, A., Arita, T., Ishida, T., and Sawa, S.
  • Journal Title: Nematology Volume: (In press)
  • Peer Reviewed
• [Journal Article] CLE6 expression recovers gibberellin deficiency to promote shoot growth in Arabidopsis. (2014)
  • Author(s): Bidadi, H.,et al.
  • Journal Title: Plant J. Volume: In press Issue: 2 Pages: 241-252
  • DOI: 10.1111/tpj.12475
  • Peer Reviewed
• [Journal Article] CLE-CLAVATA1 peptide-receptor signaling module regulates the expansion of plant root systems in a nitrogen-dependent manner (2014)
  • Author(s): Araya, T., Miyamoto, M., Mibowo, J., Suzuki, A., Kojima, S., Tsuchiya, Y. N., Sawa, S., Fukuda, H., Wiren, N., Takahashi, H.
  • Journal Title: Proc. Natl. Acad. Sci. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Identification of Japanese Lecanorchis(Orchidaceae) Species in Fruiting Stage Int (2014)
  • Author(s): Fukunaga, H., Sawa, Y., and Sawa, S.
  • Journal Title: J. Biol. Volume: 6(In press) Issue: 2
  • DOI: 10.5539/ijb.v6n2p1
  • Peer Reviewed
• [Journal Article] Evolutionarily conserved CLE peptide signaling in plant development, symbiosis, and parasitism (2013)
  • Author(s): Miyawaki, K., Tabata, R., and Sawa, S.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 16 Pages: 598-606
  • Peer Reviewed
• [Journal Article] CLE19 peptide ac tivity is regulated by SUPPRESSOR OF LLP1 1-mediated C-terminal processing in endosomes in Arabidopsis (2013)
  • Author(s): Tamaki, T., Betsuyaku, S., Hamasaki, R., Fujiwara, M., Fukao, Y., Fukuda, H., and Sawa,S.
  • Journal Title: Plant J. Volume: 6 Pages: 970-981
  • Peer Reviewed
• [Journal Article] Differential Effects of Peptides Stomagen, EPF1, and EPF2 on Activation of the MAP Kinase MPK6 and the SPCH Protein Level (2013)
  • Author(s): Jewaria, P. K., Hara, T., Tanaka, H., Kondo, T., Betsuyaku, S., Sawa, S., Sakagami, Y., Aimoto S. and Kakimoto, T.
  • Journal Title: Plant Cell Physiol Volume: 54 Pages: 1253-1262
  • NAID: 10031195179
  • Peer Reviewed
• [Journal Article] Identification of an EMS-induced causal mutation in a gene required for boron-mediated root development by low-coverage genome re-sequencing in Arabidopsis (2013)
  • Author(s): Tabata, R., Kamiya, T., Shigenobu S., Yamaguchi K., Yamada, M., Hasebe, M., Fujiwara, T., and Sawa, S.
  • Journal Title: Plant Signaling & Behavior Volume: 8
  • Peer Reviewed
• [Journal Article] Synergistic Interaction of CLAVATA1, CLAVATA2, and RECEPTOR-1 LIKE PROTEIN2 KINASE 2 in Cyst Nematode Parasitism of Arabidopsis (2013)
  • Author(s): Replogle, A., Wang, J., Paolillo, V., Smeda, J., Kinoshita, A., Durbak, A., Tax, F., Wang, X., Sawa, S., and Mitchum, M. G.
  • Journal Title: Mol Plant Microbe Interact Volume: 26 Pages: 87-96
  • Peer Reviewed
• [Journal Article] The function of CLE and other plant peptide hormones in root development (2013)
  • Author(s): Yamada, M., and Sawa, S.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 16 Pages: 1-6
  • Peer Reviewed
• [Journal Article] SUPPRESSOR OF LLP1 1-mediated C-terminal processing is critical for CLE19 peptide activity. (2013)
  • Author(s): Takayuki Tamaki
  • Journal Title: The Plant Journal Volume: 76 Issue: 6 Pages: 970-981
  • DOI: 10.1111/tpj.12349
  • Peer Reviewed
• [Presentation] 植物感染性線虫の感染分子機構の解析 (2014)
  • Author(s): 澤進一郎、光増可奈子
  • Organizer: 日本農薬学会
  • Place of Presentation: 京都大学
  • Year and Date: 2014-03-15
• [Presentation] シロイヌナズナの幹細胞活性を規定するCLV3ペプチドホルモンシグナル伝達因子の順遺伝学による網羅的探索 (2013)
  • Author(s): 澤進一郎, 志水法子, 江島千佳, 西山英孝, 石田喬志
  • Organizer: 日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] サツマイモネコブセンチュウに対する誘引忌避物質探索 (2013)
  • Author(s): 西山英孝, 肥田博隆, 戸高晃彦, 江島千佳, 相良知実, Bui Thi Ngan, 澤進一郎
  • Organizer: 日本線虫学会第21回大会
  • Place of Presentation: 唐津市民交流プラザ
• [Presentation] サツマイモネコブ線虫のエフェクタータンパク質の解析 (2013)
  • Author(s): 豊島主峰, 相良知美, 光増可奈子, 江島千佳, Bui Thi Ngan, 澤進一郎
  • Organizer: 日本線虫学会第21回大会
  • Place of Presentation: 唐津市民交流プラザ
• [Presentation] Carboxypeptidase in CLE peptide matu ration step,and CLE signaling molecu les in Arabidopsis (2013)
  • Author(s): Shinichiro Sawa
  • Organizer: 21ST Conference of the International Plant Growth Substances Association
  • Place of Presentation: The Shanghai International Conferenc e Center
• [Presentation] Involvement of plant CLE peptide sig naling in nematode infection process (2013)
  • Author(s): Ejima, B.T.Ngan, N.Shimizu, S.Sawa
  • Organizer: 21S T Conference of the International Pl ant Growth Substances Association
  • Place of Presentation: The Shanghai International Conferenc e Center
• [Presentation] Analysis of CLE peptide signaling in A rabidopsis thaliana (2013)
  • Author(s): N.Shimizu, R.Tabata, S.Shigenobu, M.Hase be, K, Yamaguchi, M.Yamada, C.Ejima, S.Sawa
  • Organizer: 21ST Conference of the International Plant Growth Substances Association
  • Place of Presentation: The Shanghai International Conference Center
• [Presentation] Carboxypeptidase in CLE peptide maturation step,and CLE signaling molecules in Arabidopsis (2013)
  • Author(s): Shinichiro Sawa
  • Organizer: 21ST Conference of the International Plant Growth Substances Association
  • Place of Presentation: The Shanghai International Conference Center
• [Presentation] シロイヌナズナの幹細胞活性を規定するCLV3ペプチドホルモンシグナル伝達因子の順遺伝学による網羅的探索 (2013)
  • Author(s): 澤進一郎,志水法子,江島千佳,西山英孝,石田喬志
  • Organizer: 日本生化学大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Participation of the cell wall interface in intercellular signaling in plants (2013)
  • Author(s): Shinichiro Sawa
  • Organizer: International Cell Wall Integrity Symposium
  • Place of Presentation: KKR Kamakura Wakamiya
• [Remarks]
  • URL: http://www.sci.kumamoto-u.ac.jp/~sawa/