Studies on chloroplast personalities by visualizing cellular signal transduction processes and photosynthesis.

• Project/Area Number: 24657036
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto Prefectural University
• Principal Investigator: SHIINA Takashi 京都府立大学, 生命環境科学研究科(系), 教授 (10206039)
• Co-Investigator(Kenkyū-buntansha): KUMAZAKI Shigeichi 京都大学, 大学院理学研究科, 准教授 (40293401)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 葉緑体 / Ca2+ / ROS / 表皮 / 免疫 / ストロミュール / 蛍光寿命顕微鏡 / シグナル / 可視化 / ミトコンドリア

Research Abstract

Different tissues contain various types of plastids that differ in function and morphology. This study revealed that plastids have distinct functions in plant immunity and Ca2+ signaling in a tissue-specific manner. Plastids in leaves and roots evoked distinct Ca2+ signals in response to biotic and abiotic stresses such as salt and osmotic stresses. Furthermore, we found that plastids in epidermis have a specific role in biosynthesis of salicylic acid in plant immunity. Interestingly, we demonstrated that Ca2+ and ROS signaling play critical roles in the induction of stromules in chloroplasts in epidermal cells of hypocotyles. These results imply that plastids in distinct tissues have specialized roles.

Research Products

• [Journal Article] Recent advances in the study of chloroplast gene expression and its evolution (2014)
  • Author(s): Yagi, Y and Shiina, T.
  • Journal Title: Front. in Plant Sci Volume: (eCollection 2014) Pages: 5-61
• [Journal Article] Calcium signaling in plant endosymbiotic organelles : mechanism and role in physiology (2014)
  • Author(s): Nomura, H. and Shiina, T.
  • Journal Title: Mol. Plant Volume: (in press)
• [Journal Article] A scalable open-source pipeline for large-scale root phenotyping (2014)
  • Author(s): Radka Slovak, Christian Göschl, Xiaoxue Su, Koji Shimotani, Takashi Shiina and Wolfgang Busch
  • Journal Title: Plant Cell Volume: (in press)
• [Journal Article] Recent advances in the study of chloroplast gene expression and its evolution. (2014)
  • Author(s): Yagi, Y and Shiina, T.
  • Journal Title: Front. in Plant Sci. Volume: 5 Pages: 61-61
  • Peer Reviewed
• [Journal Article] Calcium signaling in plant endosymbiotic organelles: mechanism and role in physiology. (2014)
  • Author(s): Nomura, H. and Shiina, T.
  • Journal Title: Mol. Plant Volume: 未定
  • Peer Reviewed
• [Journal Article] A scalable open-source pipeline for large-scale root phenotyping. (2014)
  • Author(s): 52) Radka Slovak1, Christian Göschl1, Xiaoxue Su, Koji Shimotani, Takashi Shiina and Wolfgang Busch
  • Journal Title: Plant Cell Volume: 未定
  • Peer Reviewed
• [Journal Article] Chloroplast envelope localization of EDS5, an essential factor for salicylic acid biosynthesis in Arabidopsis thaliana (2013)
  • Author(s): Yamasaki K, Motomura Y, Yagi Y, Nomura H, Kikuchi S, Nakai M, Shiina T.
  • Journal Title: Plant Signal Behav Volume: 8
• [Journal Article] Overproduction of hyperthermostable β-1,4-endoglucanase from the archaeon Pyrococcus horikoshii by tobacco chloroplast engineering (2013)
  • Author(s): Nakahira Y, Ishikawa K, Tanaka K, Tozawa Y, Shiina T.
  • Journal Title: Biosci Biotechnol Biochem Volume: 77 Pages: 2140-3
  • NAID: 130003381889
• [Journal Article] Chloroplast envelope localization of EDS5, an essential factor for salicylic acid biosynthesis in Arabidopsis thaliana. (2013)
  • Author(s): Yamasaki K, Motomura Y, Yagi Y, Nomura H, Kikuchi S, Nakai M, Shiina T.
  • Journal Title: Plant Signal Behav. Volume: 8 Issue: 4 Pages: e23603-e23603
  • DOI: 10.4161/psb.23603
  • Peer Reviewed
• [Journal Article] Overproduction of hyperthermostable β-1,4-endoglucanase from the archaeon Pyrococcus horikoshii by tobacco chloroplast engineering. (2013)
  • Author(s): Nakahira Y, Ishikawa K, Tanaka K, Tozawa Y, Shiina T.
  • Journal Title: Biosci Biotechnol Biochem. Volume: 77 Pages: 2140-2143
  • NAID: 130003381889
  • Peer Reviewed
• [Journal Article] Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis. (2013)
  • Author(s): Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH.
  • Journal Title: Plant J. Volume: 2013 73 Issue: 5 Pages: 761-75
  • DOI: 10.1111/tpj.12069
  • Peer Reviewed
• [Journal Article] Transformation of thylakoid membranes during differentiation from vegetative cell into heterocyst visualized by microscopic spectral imaging. (2013)
  • Author(s): Kumazaki S, Akari M, Hasegawa M.
  • Journal Title: Plant Physiol. Volume: 161 Issue: 3 Pages: 1321-1333
  • DOI: 10.1104/pp.112.206680
  • Peer Reviewed
• [Journal Article] Functional characterization of ObgC in ribosome biogenesis during chloroplast development. (2012)
  • Author(s): Bang WY, Chen J, Jeong IS, Kim SW, Kim CW, Jung HS, Lee KH, Kweon HS, Yoko I, Shiina T, Bahk JD.
  • Journal Title: Plant J. Volume: 71 Issue: 1 Pages: 122-34
  • DOI: 10.1111/j.1365-313x.2012.04976.x
  • Peer Reviewed
• [Journal Article] Eukaryotic-type plastid nucleoid protein pTAC3 is essential for transcription by the bacterial-type plastid RNA polymerase. (2012)
  • Author(s): Yagi Y, Ishizaki Y, Nakahira Y, Tozawa Y, Shiina T.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 109 Issue: 19 Pages: 7541-6
  • DOI: 10.1073/pnas.1119403109
  • Peer Reviewed
• [Journal Article] Chloroplast-mediated activation of plant immune signalling in Arabidopsis. (2012)
  • Author(s): Nomura H, Komori T, Uemura S, Kanda Y, Shimotani K, Nakai K, Furuichi T, Takebayashi K, Sugimoto T, Sano S, Suwastika IN, Fukusaki E, Yoshioka H, Nakahira Y, Shiina T.
  • Journal Title: Nat Commun.26 Volume: 3 Issue: 1 Pages: 926-926
  • DOI: 10.1038/ncomms1926
  • Peer Reviewed
• [Journal Article] Evolutionary aspects of plastid proteins involved in transcription: The transcription of a tiny genome is mediated by a complicated machinery. (2012)
  • Author(s): Yagi Y, Shiina T.
  • Journal Title: Transcription Volume: 3 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Transformation of thylakoid membranes during differentiation from vegetative cell into heterocyst visualized by microscopic spectral imaging
  • Author(s): Kumazaki S, Akari M, Hasegawa M.
  • Journal Title: Plant Physiol Volume: 161 Pages: 1321-1333
• [Presentation] AMPが誘導する病害抵抗瀬遺伝子発現と光合成電子伝達 (2014)
  • Author(s): 青山真夕、中井香奈、下谷紘司、佐野智、椎名隆
  • Organizer: 第55回日本植物生理学会
  • Place of Presentation: 富山
• [Presentation] 葉緑体Ca^<2+>センサーCASを介した防御応答遺伝子発現の制御 (2014)
  • Author(s): 下谷 紘司、青山 真夕、中井 香奈、佐野智、椎名 隆
  • Organizer: 第55回日本植物生理学会
  • Place of Presentation: 富山
• [Presentation] ミトコンドリア局在機械受容チャネルMSL1の機能解析 (2014)
  • Author(s): 艾原佐紀,原田尚実,市川美恵,古市卓也、椎名隆
  • Organizer: 第55回日本植物生理学会
  • Place of Presentation: 富山
• [Presentation] PAMPが誘導する病害抵抗瀬遺伝子発現と光合成電子伝達 (2014)
  • Author(s): 青山真夕、中井香奈、下谷紘司、佐野智、椎名隆
  • Organizer: 第55回日本植物生理学会
  • Place of Presentation: 富山
• [Presentation] 葉緑体Ca2+センサーCASを介した防御応答遺伝子発現の制御 (2014)
  • Author(s): 下谷 紘司、青山 真夕、中井 香奈、佐野 智、椎名 隆
  • Organizer: 第55回日本植物生理学会
  • Place of Presentation: 富山
• [Presentation] 葉緑体光転写制御におけるコムギ葉緑体RNAポリメラーゼ複合体のDNA結合動態の解析 (2013)
  • Author(s): 本村幸也,八木祐介,中平洋一,戸澤譲,椎名隆
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都
• [Presentation] ウルシノキのトランスクリプトーム解析 (2013)
  • Author(s): 石崎陽子,岡本嘉明,佐藤貴彦,大藪泰,椎名隆
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都
• [Presentation] ミトコンドリアCa^<2+> センサータンパク質AtMICU1の機能解析 (2013)
  • Author(s): 山口咲希,八木祐介,椎名隆
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都
• [Presentation] チラコイド膜CASタンパク質が制御する防御関連転写因子群の解析 (2013)
  • Author(s): 下谷紘司,神田ゆい,中平洋一,椎名隆
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都
• [Presentation] 光条件に依存する植物病理応答遺伝子の発現解析 (2013)
  • Author(s): 中井香奈,金麗花,中平洋一,椎名隆
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都
• [Presentation] 植物免疫における葉緑体ラジカル制御タンパク質CASの下流で働く因子の単離と解析 (2013)
  • Author(s): 野村裕也,藤原正幸,深尾陽一朗,椎名隆,吉岡博文
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都
• [Presentation] Chloroplast Ca^<2+> and plant immunity (2013)
  • Author(s): Shiina, T.
  • Organizer: 3rd International Symposium on Chloroplast Genomics and Genetic
  • Place of Presentation: ニューブルンスイック 米国
• [Presentation] Functional Characterization of Mitochondrial Mechanosensitive Channel MSL1 in Arabidopsis thaliana (2013)
  • Author(s): Yomogihara, S., Harada, N., Asakura, C., Yamaguchi, S., Ichikawa, M., Furuichi, T., Shiina, T.
  • Organizer: 第24回国際シロイヌナズナ研究会議
  • Place of Presentation: シドニー オーストラリア
• [Presentation] Control of defense gene expression via chloroplast Ca^<2+> sensor protein CAS in Arabidopsis (2013)
  • Author(s): Shimotani1, K., Nakai1, K., Nomura, H., Yoshioka, H., Shiina, T.
  • Organizer: 第24回国際シロイヌナズナ研究会議
  • Place of Presentation: シドニー オーストラリア
• [Presentation] ミトコンドリア機械受容チャネルMSL1の機能解析 (2013)
  • Author(s): 艾原佐紀,原田尚実,市川美恵,椎名隆
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 札幌
• [Presentation] 葉緑体accD 遺伝子プロモータの進化 (2013)
  • Author(s): 石崎陽子,木寅翔太,新村周一,椎名隆
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 札幌
• [Presentation] 葉緑体Ca^<2+>センサーCASによる植物の免疫応答制御の解析 (2013)
  • Author(s): 下谷紘司,中井香奈,山崎加奈子,佐野智、椎名隆
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 札幌
• [Presentation] 葉緑体ストロミュールのABAによる誘導機構 (2013)
  • Author(s): 椎名隆、金麗花
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 札幌
• [Presentation] Role of chloroplasts in plant defense responses (2013)
  • Author(s): Shiina, T.
  • Organizer: International Closing Meeting of the Research Unit 804‚ Retrograde Signaling in Plants
  • Place of Presentation: ミュンヘン ドイツ
• [Presentation] A role for chloroplast Ca^<2+> sensor protein CAS in regulation of intracellular Ca2+ signaling and plant defense responses (2013)
  • Author(s): Shiina, T.
  • Organizer: Indo-Japan Joint Workshop on "Signal sensing and transduction in photosynthetic organisms-from cyanobacteria to land plants"
  • Place of Presentation: ハイデラバード インド
• [Presentation] Chloroplast Ca2+ and plant immunity (2013)
  • Author(s): Shiina, T.
  • Organizer: 3rd International Symposium on Chloroplast Genomics and Genetic.
  • Place of Presentation: ニューブルンスイック 米国
  • Invited
• [Presentation] Role of chloroplasts in plant defense responses (2013)
  • Author(s): Shiina, T.
  • Organizer: International Closing Meeting of the Research Unit 804 &#8218;Retrograde Signaling in Plants’
  • Place of Presentation: ミュンヘン ドイツ
  • Invited
• [Presentation] A role for chloroplast Ca2+ sensor protein CAS in regulation of intracellular Ca2+ signaling and plant defense responses (2013)
  • Author(s): Shiina, T.
  • Organizer: Indo-Japan Joint Workshop on "Signal sensing and transduction in photosynthetic organisms-from cyanobacteria to land plants"
  • Place of Presentation: ハイデラバード インド
  • Invited
• [Presentation] unctional Characterization of Mitochondrial Mechanosensitive Channel MSL1 in Arabidopsis thaliana. (2013)
  • Author(s): Yomogihara, S., Harada, N., Asakura, C., Yamaguchi, S., Ichikawa, M., Furuichi, T., Shiina, T.
  • Organizer: 第24回国際シロイヌナズナ研究会議
  • Place of Presentation: シドニー オーストラリア
• [Presentation] Control of defense gene expression via chloroplast Ca2+ sensor protein CAS in Arabidopsis. (2013)
  • Author(s): Shimotani1, K., Nakai1, K., Nomura, H., Yoshioka, H., Shiina, T.
  • Organizer: 第24回国際シロイヌナズナ研究会議
  • Place of Presentation: シドニー オーストラリア
• [Presentation] 葉緑体accD遺伝子プロモータの進化 (2013)
  • Author(s): 石崎陽子，木寅翔太，新村周一，椎名隆
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 札幌
• [Presentation] 葉緑体Ca2+センサーCASによる植物の免疫応答制御の解析 (2013)
  • Author(s): 下谷紘司，中井香奈,山崎加奈子,佐野智、椎名隆
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 札幌
• [Presentation] 植物の細胞内シグナル伝達系と共生オルガネラ (2013)
  • Author(s): 椎名隆
  • Organizer: 第54回植物生理学会
  • Place of Presentation: 岡山市
  • Invited
• [Presentation] ミトコンドリアのCa2+センサータンパク質候補AtMICU1の機能解析 (2013)
  • Author(s): 浅倉千洋
  • Organizer: 第54回植物生理学会
  • Place of Presentation: 岡山市
• [Presentation] 葉緑体CASタンパク質による防御応答遺伝子の発現制御 (2013)
  • Author(s): 下谷紘司、神田ゆい、中井香奈、山崎加奈子、椎名隆
  • Organizer: 第54回植物生理学会
  • Place of Presentation: 岡山市
• [Presentation] ミトコンドリア機械受容チャネルMSL1の機能解析 (2013)
  • Author(s): 𦫿原佐紀、原田尚実、市川美恵、古市卓也、椎名隆
  • Organizer: 第54回植物生理学会
  • Place of Presentation: 岡山市
• [Presentation] 葉緑体ストレス応答プロモータpsbD KRPの構造とストレス応答性の進化 (2013)
  • Author(s): 木寅 翔太、椎名隆
  • Organizer: 第54回植物生理学会
  • Place of Presentation: 岡山市
• [Presentation] Crosstalk between Plant Immunity and Chloroplasts (2013)
  • Author(s): 椎名隆
  • Organizer: 29th IPSR International Symposium and 5th Symposium on Plant Sciences
  • Place of Presentation: 倉敷市
  • Invited
• [Presentation] ウルシノキのトランス クリプトーム解析 (2012)
  • Author(s): 石崎 陽子, 岡本 嘉明, 佐藤 貴彦, 大藪 泰, 椎 名 隆
  • Organizer: 第76回植物学会
  • Place of Presentation: 姫路市
• [Presentation] 光による防御応答遺伝 子の発現制御 (2012)
  • Author(s): 中井 香奈, 佐野 智, 椎 名 隆
  • Organizer: 第76回植物学会
  • Place of Presentation: 姫路市
• [Presentation] 葉緑体CAS タンパク質 による防御応答関連転 写因子群の発現制御 (2012)
  • Author(s): 下谷 紘司, 神田 ゆい, 椎名 隆
  • Organizer: 第76回植物学会
  • Place of Presentation: 姫路市
• [Presentation] 葉緑体psbD 光応答プロモーターの光応答及びス トレス応答機能について (2012)
  • Author(s): 木寅 翔太, 椎名 隆, 新村 修一
  • Organizer: 第76回植物学会
  • Place of Presentation: 姫路市
• [Presentation] Light-dependent Expression of PAMP-induced Defense Genes (2012)
  • Author(s): K. Nakai, S. Sano and T. Shin
  • Organizer: 10th International Plant Molecular Biology Congress
  • Place of Presentation: 済州島
• [Presentation] Transcription Factors Under the Control of Chloroplast-derived Immune Signalling Arabidopsis thaliana (2012)
  • Author(s): K. Shimotani, Y . Kanda and T. Shiina
  • Organizer: 10th International Plant Molecular Biology Congress
  • Place of Presentation: 済州島
• [Presentation] Evolutionary Analysis of Plastid Psbd Light-responsive Promoters and Their Stress Response in Land Plants (2012)
  • Author(s): S. Kitora, S. Shimmura, M. Nozoe and T. Shiina
  • Organizer: 10th International Plant Molecular Biology Congress
  • Place of Presentation: 済州島
• [Presentation] Chloroplast Ca2+ and Plant Immunity (2012)
  • Author(s): T. Shiina
  • Organizer: 3rd International Symposium on Chloroplast Genomics and Genetic Engineerin
  • Place of Presentation: アメリカ New Brucswick市