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Studies on chloroplast personalities by visualizing cellular signal transduction processes and photosynthesis.

Research Project

Project/Area Number 24657036
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto Prefectural University

Principal Investigator

SHIINA Takashi  京都府立大学, 生命環境科学研究科(系), 教授 (10206039)

Co-Investigator(Kenkyū-buntansha) KUMAZAKI Shigeichi  京都大学, 大学院理学研究科, 准教授 (40293401)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords葉緑体 / Ca2+ / ROS / 表皮 / 免疫 / ストロミュール / 蛍光寿命顕微鏡 / シグナル / 可視化 / ミトコンドリア
Research Abstract

Different tissues contain various types of plastids that differ in function and morphology. This study revealed that plastids have distinct functions in plant immunity and Ca2+ signaling in a tissue-specific manner. Plastids in leaves and roots evoked distinct Ca2+ signals in response to biotic and abiotic stresses such as salt and osmotic stresses. Furthermore, we found that plastids in epidermis have a specific role in biosynthesis of salicylic acid in plant immunity. Interestingly, we demonstrated that Ca2+ and ROS signaling play critical roles in the induction of stromules in chloroplasts in epidermal cells of hypocotyles. These results imply that plastids in distinct tissues have specialized roles.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (58 results)

All 2014 2013 2012 Other

All Journal Article (17 results) (of which Peer Reviewed: 11 results) Presentation (41 results) (of which Invited: 5 results)

  • [Journal Article] Recent advances in the study of chloroplast gene expression and its evolution2014

    • Author(s)
      Yagi, Y and Shiina, T.
    • Journal Title

      Front. in Plant Sci

      Volume: (eCollection 2014) Pages: 5-61

    • Related Report
      2013 Final Research Report
  • [Journal Article] Calcium signaling in plant endosymbiotic organelles : mechanism and role in physiology2014

    • Author(s)
      Nomura, H. and Shiina, T.
    • Journal Title

      Mol. Plant

      Volume: (in press)

    • Related Report
      2013 Final Research Report
  • [Journal Article] A scalable open-source pipeline for large-scale root phenotyping2014

    • Author(s)
      Radka Slovak, Christian Göschl, Xiaoxue Su, Koji Shimotani, Takashi Shiina and Wolfgang Busch
    • Journal Title

      Plant Cell

      Volume: (in press)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Recent advances in the study of chloroplast gene expression and its evolution.2014

    • Author(s)
      Yagi, Y and Shiina, T.
    • Journal Title

      Front. in Plant Sci.

      Volume: 5 Pages: 61-61

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calcium signaling in plant endosymbiotic organelles: mechanism and role in physiology.2014

    • Author(s)
      Nomura, H. and Shiina, T.
    • Journal Title

      Mol. Plant

      Volume: 未定

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A scalable open-source pipeline for large-scale root phenotyping.2014

    • Author(s)
      52) Radka Slovak1, Christian Göschl1, Xiaoxue Su, Koji Shimotani, Takashi Shiina and Wolfgang Busch
    • Journal Title

      Plant Cell

      Volume: 未定

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplast envelope localization of EDS5, an essential factor for salicylic acid biosynthesis in Arabidopsis thaliana2013

    • Author(s)
      Yamasaki K, Motomura Y, Yagi Y, Nomura H, Kikuchi S, Nakai M, Shiina T.
    • Journal Title

      Plant Signal Behav

      Volume: 8

    • Related Report
      2013 Final Research Report
  • [Journal Article] Overproduction of hyperthermostable β-1,4-endoglucanase from the archaeon Pyrococcus horikoshii by tobacco chloroplast engineering2013

    • Author(s)
      Nakahira Y, Ishikawa K, Tanaka K, Tozawa Y, Shiina T.
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 77 Pages: 2140-3

    • NAID

      130003381889

    • Related Report
      2013 Final Research Report
  • [Journal Article] Chloroplast envelope localization of EDS5, an essential factor for salicylic acid biosynthesis in Arabidopsis thaliana.2013

    • Author(s)
      Yamasaki K, Motomura Y, Yagi Y, Nomura H, Kikuchi S, Nakai M, Shiina T.
    • Journal Title

      Plant Signal Behav.

      Volume: 8 Issue: 4 Pages: e23603-e23603

    • DOI

      10.4161/psb.23603

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Overproduction of hyperthermostable β-1,4-endoglucanase from the archaeon Pyrococcus horikoshii by tobacco chloroplast engineering.2013

    • Author(s)
      Nakahira Y, Ishikawa K, Tanaka K, Tozawa Y, Shiina T.
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 77 Pages: 2140-2143

    • NAID

      130003381889

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis.2013

    • Author(s)
      Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH.
    • Journal Title

      Plant J.

      Volume: 2013 73 Issue: 5 Pages: 761-75

    • DOI

      10.1111/tpj.12069

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transformation of thylakoid membranes during differentiation from vegetative cell into heterocyst visualized by microscopic spectral imaging.2013

    • Author(s)
      Kumazaki S, Akari M, Hasegawa M.
    • Journal Title

      Plant Physiol.

      Volume: 161 Issue: 3 Pages: 1321-1333

    • DOI

      10.1104/pp.112.206680

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of ObgC in ribosome biogenesis during chloroplast development.2012

    • Author(s)
      Bang WY, Chen J, Jeong IS, Kim SW, Kim CW, Jung HS, Lee KH, Kweon HS, Yoko I, Shiina T, Bahk JD.
    • Journal Title

      Plant J.

      Volume: 71 Issue: 1 Pages: 122-34

    • DOI

      10.1111/j.1365-313x.2012.04976.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Eukaryotic-type plastid nucleoid protein pTAC3 is essential for transcription by the bacterial-type plastid RNA polymerase.2012

    • Author(s)
      Yagi Y, Ishizaki Y, Nakahira Y, Tozawa Y, Shiina T.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 109 Issue: 19 Pages: 7541-6

    • DOI

      10.1073/pnas.1119403109

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chloroplast-mediated activation of plant immune signalling in Arabidopsis.2012

    • Author(s)
      Nomura H, Komori T, Uemura S, Kanda Y, Shimotani K, Nakai K, Furuichi T, Takebayashi K, Sugimoto T, Sano S, Suwastika IN, Fukusaki E, Yoshioka H, Nakahira Y, Shiina T.
    • Journal Title

      Nat Commun.26

      Volume: 3 Issue: 1 Pages: 926-926

    • DOI

      10.1038/ncomms1926

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evolutionary aspects of plastid proteins involved in transcription: The transcription of a tiny genome is mediated by a complicated machinery.2012

    • Author(s)
      Yagi Y, Shiina T.
    • Journal Title

      Transcription

      Volume: 3 Pages: 1-5

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transformation of thylakoid membranes during differentiation from vegetative cell into heterocyst visualized by microscopic spectral imaging

    • Author(s)
      Kumazaki S, Akari M, Hasegawa M.
    • Journal Title

      Plant Physiol

      Volume: 161 Pages: 1321-1333

    • Related Report
      2013 Final Research Report
  • [Presentation] AMPが誘導する病害抵抗瀬遺伝子発現と光合成電子伝達2014

    • Author(s)
      青山真夕、中井香奈、下谷紘司、佐野智、椎名隆
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山
    • Related Report
      2013 Final Research Report
  • [Presentation] 葉緑体Ca^<2+>センサーCASを介した防御応答遺伝子発現の制御2014

    • Author(s)
      下谷 紘司、青山 真夕、中井 香奈、佐野智、椎名 隆
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山
    • Related Report
      2013 Final Research Report
  • [Presentation] ミトコンドリア局在機械受容チャネルMSL1の機能解析2014

    • Author(s)
      艾原佐紀,原田尚実,市川美恵,古市卓也、椎名隆
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] PAMPが誘導する病害抵抗瀬遺伝子発現と光合成電子伝達2014

    • Author(s)
      青山真夕、中井香奈、下谷紘司、佐野智、椎名隆
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] 葉緑体Ca2+センサーCASを介した防御応答遺伝子発現の制御2014

    • Author(s)
      下谷 紘司、青山 真夕、中井 香奈、佐野 智、椎名 隆
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] 葉緑体光転写制御におけるコムギ葉緑体RNAポリメラーゼ複合体のDNA結合動態の解析2013

    • Author(s)
      本村幸也,八木祐介,中平洋一,戸澤譲,椎名隆
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] ウルシノキのトランスクリプトーム解析2013

    • Author(s)
      石崎陽子,岡本嘉明,佐藤貴彦,大藪泰,椎名隆
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] ミトコンドリアCa^<2+> センサータンパク質AtMICU1の機能解析2013

    • Author(s)
      山口咲希,八木祐介,椎名隆
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] チラコイド膜CASタンパク質が制御する防御関連転写因子群の解析2013

    • Author(s)
      下谷紘司,神田ゆい,中平洋一,椎名隆
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 光条件に依存する植物病理応答遺伝子の発現解析2013

    • Author(s)
      中井香奈,金麗花,中平洋一,椎名隆
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物免疫における葉緑体ラジカル制御タンパク質CASの下流で働く因子の単離と解析2013

    • Author(s)
      野村裕也,藤原正幸,深尾陽一朗,椎名隆,吉岡博文
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] Chloroplast Ca^<2+> and plant immunity2013

    • Author(s)
      Shiina, T.
    • Organizer
      3rd International Symposium on Chloroplast Genomics and Genetic
    • Place of Presentation
      ニューブルンスイック 米国
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional Characterization of Mitochondrial Mechanosensitive Channel MSL1 in Arabidopsis thaliana2013

    • Author(s)
      Yomogihara, S., Harada, N., Asakura, C., Yamaguchi, S., Ichikawa, M., Furuichi, T., Shiina, T.
    • Organizer
      第24回国際シロイヌナズナ研究会議
    • Place of Presentation
      シドニー オーストラリア
    • Related Report
      2013 Final Research Report
  • [Presentation] Control of defense gene expression via chloroplast Ca^<2+> sensor protein CAS in Arabidopsis2013

    • Author(s)
      Shimotani1, K., Nakai1, K., Nomura, H., Yoshioka, H., Shiina, T.
    • Organizer
      第24回国際シロイヌナズナ研究会議
    • Place of Presentation
      シドニー オーストラリア
    • Related Report
      2013 Final Research Report
  • [Presentation] ミトコンドリア機械受容チャネルMSL1の機能解析2013

    • Author(s)
      艾原佐紀,原田尚実,市川美恵,椎名隆
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 葉緑体accD 遺伝子プロモータの進化2013

    • Author(s)
      石崎陽子,木寅翔太,新村周一,椎名隆
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] 葉緑体Ca^<2+>センサーCASによる植物の免疫応答制御の解析2013

    • Author(s)
      下谷紘司,中井香奈,山崎加奈子,佐野智、椎名隆
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] 葉緑体ストロミュールのABAによる誘導機構2013

    • Author(s)
      椎名隆、金麗花
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Role of chloroplasts in plant defense responses2013

    • Author(s)
      Shiina, T.
    • Organizer
      International Closing Meeting of the Research Unit 804‚ Retrograde Signaling in Plants
    • Place of Presentation
      ミュンヘン ドイツ
    • Related Report
      2013 Final Research Report
  • [Presentation] A role for chloroplast Ca^<2+> sensor protein CAS in regulation of intracellular Ca2+ signaling and plant defense responses2013

    • Author(s)
      Shiina, T.
    • Organizer
      Indo-Japan Joint Workshop on "Signal sensing and transduction in photosynthetic organisms-from cyanobacteria to land plants"
    • Place of Presentation
      ハイデラバード インド
    • Related Report
      2013 Final Research Report
  • [Presentation] Chloroplast Ca2+ and plant immunity2013

    • Author(s)
      Shiina, T.
    • Organizer
      3rd International Symposium on Chloroplast Genomics and Genetic.
    • Place of Presentation
      ニューブルンスイック 米国
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Role of chloroplasts in plant defense responses2013

    • Author(s)
      Shiina, T.
    • Organizer
      International Closing Meeting of the Research Unit 804 &#8218;Retrograde Signaling in Plants’
    • Place of Presentation
      ミュンヘン ドイツ
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] A role for chloroplast Ca2+ sensor protein CAS in regulation of intracellular Ca2+ signaling and plant defense responses2013

    • Author(s)
      Shiina, T.
    • Organizer
      Indo-Japan Joint Workshop on "Signal sensing and transduction in photosynthetic organisms-from cyanobacteria to land plants"
    • Place of Presentation
      ハイデラバード インド
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] unctional Characterization of Mitochondrial Mechanosensitive Channel MSL1 in Arabidopsis thaliana.2013

    • Author(s)
      Yomogihara, S., Harada, N., Asakura, C., Yamaguchi, S., Ichikawa, M., Furuichi, T., Shiina, T.
    • Organizer
      第24回国際シロイヌナズナ研究会議
    • Place of Presentation
      シドニー オーストラリア
    • Related Report
      2013 Annual Research Report
  • [Presentation] Control of defense gene expression via chloroplast Ca2+ sensor protein CAS in Arabidopsis.2013

    • Author(s)
      Shimotani1, K., Nakai1, K., Nomura, H., Yoshioka, H., Shiina, T.
    • Organizer
      第24回国際シロイヌナズナ研究会議
    • Place of Presentation
      シドニー オーストラリア
    • Related Report
      2013 Annual Research Report
  • [Presentation] 葉緑体accD遺伝子プロモータの進化2013

    • Author(s)
      石崎陽子,木寅翔太,新村周一,椎名隆
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] 葉緑体Ca2+センサーCASによる植物の免疫応答制御の解析2013

    • Author(s)
      下谷紘司,中井香奈,山崎加奈子,佐野智、椎名隆
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物の細胞内シグナル伝達系と共生オルガネラ2013

    • Author(s)
      椎名隆
    • Organizer
      第54回植物生理学会
    • Place of Presentation
      岡山市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] ミトコンドリアのCa2+センサータンパク質候補AtMICU1の機能解析2013

    • Author(s)
      浅倉千洋
    • Organizer
      第54回植物生理学会
    • Place of Presentation
      岡山市
    • Related Report
      2012 Research-status Report
  • [Presentation] 葉緑体CASタンパク質による防御応答遺伝子の発現制御2013

    • Author(s)
      下谷紘司、神田ゆい、中井香奈、山崎加奈子、椎名隆
    • Organizer
      第54回植物生理学会
    • Place of Presentation
      岡山市
    • Related Report
      2012 Research-status Report
  • [Presentation] ミトコンドリア機械受容チャネルMSL1の機能解析2013

    • Author(s)
      𦫿原佐紀、原田尚実、市川美恵、古市卓也、椎名隆
    • Organizer
      第54回植物生理学会
    • Place of Presentation
      岡山市
    • Related Report
      2012 Research-status Report
  • [Presentation] 葉緑体ストレス応答プロモータpsbD KRPの構造とストレス応答性の進化2013

    • Author(s)
      木寅 翔太、椎名隆
    • Organizer
      第54回植物生理学会
    • Place of Presentation
      岡山市
    • Related Report
      2012 Research-status Report
  • [Presentation] Crosstalk between Plant Immunity and Chloroplasts2013

    • Author(s)
      椎名隆
    • Organizer
      29th IPSR International Symposium and 5th Symposium on Plant Sciences
    • Place of Presentation
      倉敷市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] ウルシノキのトランス クリプトーム解析2012

    • Author(s)
      石崎 陽子, 岡本 嘉明, 佐藤 貴彦, 大藪 泰, 椎 名 隆
    • Organizer
      第76回植物学会
    • Place of Presentation
      姫路市
    • Related Report
      2012 Research-status Report
  • [Presentation] 光による防御応答遺伝 子の発現制御2012

    • Author(s)
      中井 香奈, 佐野 智, 椎 名 隆
    • Organizer
      第76回植物学会
    • Place of Presentation
      姫路市
    • Related Report
      2012 Research-status Report
  • [Presentation] 葉緑体CAS タンパク質 による防御応答関連転 写因子群の発現制御2012

    • Author(s)
      下谷 紘司, 神田 ゆい, 椎名 隆
    • Organizer
      第76回植物学会
    • Place of Presentation
      姫路市
    • Related Report
      2012 Research-status Report
  • [Presentation] 葉緑体psbD 光応答プロモーターの光応答及びス トレス応答機能について2012

    • Author(s)
      木寅 翔太, 椎名 隆, 新村 修一
    • Organizer
      第76回植物学会
    • Place of Presentation
      姫路市
    • Related Report
      2012 Research-status Report
  • [Presentation] Light-dependent Expression of PAMP-induced Defense Genes2012

    • Author(s)
      K. Nakai, S. Sano and T. Shin
    • Organizer
      10th International Plant Molecular Biology Congress
    • Place of Presentation
      済州島
    • Related Report
      2012 Research-status Report
  • [Presentation] Transcription Factors Under the Control of Chloroplast-derived Immune Signalling Arabidopsis thaliana2012

    • Author(s)
      K. Shimotani, Y . Kanda and T. Shiina
    • Organizer
      10th International Plant Molecular Biology Congress
    • Place of Presentation
      済州島
    • Related Report
      2012 Research-status Report
  • [Presentation] Evolutionary Analysis of Plastid Psbd Light-responsive Promoters and Their Stress Response in Land Plants2012

    • Author(s)
      S. Kitora, S. Shimmura, M. Nozoe and T. Shiina
    • Organizer
      10th International Plant Molecular Biology Congress
    • Place of Presentation
      済州島
    • Related Report
      2012 Research-status Report
  • [Presentation] Chloroplast Ca2+ and Plant Immunity2012

    • Author(s)
      T. Shiina
    • Organizer
      3rd International Symposium on Chloroplast Genomics and Genetic Engineerin
    • Place of Presentation
      アメリカ New Brucswick市
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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