| Project/Area Number |
24657038
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hosei University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | イネ細胞 / タバコモザイクウイルス / キュウリモザイクウイルス / RNAサイレンシング / サプレッション / エレクトロポレーション / ウイルス生産の抑制解除機構 / マイクロRNA |
|---|
| Research Abstract |
We have shown previously that the propagation of TMV was not observed in rice protoplasts, when TMV-RNA was solely introduced into them. However, TMV propagation was observed, when both TMV-RNA and CMV-RNA were introduced into rice protoplasts. This observation may be interpreted from the viewpoints of microRNA functioning. Generally speaking, it is said that upon virus infection, RNA silencing functions; however, in some of virus products, factors that suppress RNA silencing function upon virus infection. Thus, in this study one of such factors is supposed to be a gene product of 2b of CMV. It is inferred that certain factors in rice cells have been identified that interact with the 2b protein.
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