Elucidation of the virus propagation-interference mechanism in plant cells and search for suppressing factors of this process

• Project/Area Number: 24657038
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Hosei University
• Principal Investigator: NAGATA Toshiyuki 法政大学, 生命科学部, 教授 (10012519)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: イネ細胞 / タバコモザイクウイルス / キュウリモザイクウイルス / RNAサイレンシング / サプレッション / エレクトロポレーション / ウイルス生産の抑制解除機構 / マイクロRNA

Research Abstract

We have shown previously that the propagation of TMV was not observed in rice protoplasts, when TMV-RNA was solely introduced into them. However, TMV propagation was observed, when both TMV-RNA and CMV-RNA were introduced into rice protoplasts. This observation may be interpreted from the viewpoints of microRNA functioning. Generally speaking, it is said that upon virus infection, RNA silencing functions; however, in some of virus products, factors that suppress RNA silencing function upon virus infection. Thus, in this study one of such factors is supposed to be a gene product of 2b of CMV. It is inferred that certain factors in rice cells have been identified that interact with the 2b protein.

Research Products

• [Journal Article] Paulownia tomentosa. A Chinese plant in Japan (2013)
  • Author(s): Nagata, Toshiyuki, DuVal, A., Lack, W.W., Newbitt, M., Schmull, M., Crane, P.R.
  • Journal Title: Curtis's Botanical Magazine Volume: Vol. 30 Pages: 261-274
  • Peer Reviewed
• [Journal Article] Ginkgo biloba. Connections with people and art across thousand years (2013)
  • Author(s): Crane, P.R., Nagata, Toshiyuki, Murata, J., Ohi-Toma, T., DuVal, A., Newvitt, M., Jarvis, C.
  • Journal Title: Curis's Botanical Magazine Volume: Vol. 30 Pages: 239-260
  • Peer Reviewed
• [Journal Article] An unusual xylotheques with plant illustrations from early Meiji Japan (2013)
  • Author(s): Nagata, Toshiyuki, DuVal, A., Lack, H.W., Loudon, G., Nesbitt, M., Schmull, M., Crane, P.R.
  • Journal Title: Economic Botany Volume: Vol. 67 Pages: 87-92
  • Peer Reviewed
• [Journal Article] 小石川、キュー、ベルリン―ダーレム植物園をつなぐ「加藤竹斎」図版 (2013)
  • Author(s): 長田敏行、DuVal, A., 東馬哲雄、邑田仁、Crane, P.
  • Journal Title: 日本植物園協会誌 Volume: Vol. 48 Pages: 112-113
  • Peer Reviewed
• [Journal Article] Paulownia tomentosa. A Chinese plant in Japan. (2013)
  • Author(s): Nagata, T., DuVal, A., Lack, W.W., Nesbitt, M., Schmull, M, P.R. Crane
  • Journal Title: Curtis's Botanical Magazine Volume: 30 Pages: 261-274
  • Peer Reviewed
• [Journal Article] Ginkgo biloba. Connection with people and art across thousand years. (2013)
  • Author(s): Crane, P.R., Nagata, T., Murata, J., Ohi-Toma, T., DuVal, A., Mesbitt, M., Jarvis, C.
  • Journal Title: Curtis's Botanical Magazine Volume: 30 Pages: 239-260
  • Peer Reviewed
• [Journal Article] An unusual xylotheque with plant illustrations from early Meiji Japany me (2013)
  • Author(s): Nagata, T., DuVal, A., Lack, H.W., Loudon, G., Nesbitt, M., Schmull, M., Crane, P.R.
  • Journal Title: Economic Botany Volume: 67 Issue: 2 Pages: 87-92
  • DOI: 10.1007/s12231-013-9227-6
  • Peer Reviewed
• [Journal Article] 小石川、キュー、ベルリン-ダーレム植物園をつなぐ「加藤竹斎」図版 (2013)
  • Author(s): 長田敏行、DuVal, A., 東馬哲夫、邑田仁、Crane, P.R.
  • Journal Title: 日本植物園協会誌 Volume: 48 Pages: 112-113
  • Peer Reviewed
• [Journal Article] Role of actin microfilaments in phragmoplast guidance to the cortical division zone (2012)
  • Author(s): Sano, T., Hayashi, T., Kutsuna, N., Nagata, Toshiyuki, Hasezawa, S.
  • Journal Title: Current Topics in Plant Science Volume: Vol. 13 Pages: 87-94
  • Peer Reviewed
• [Journal Article] Light as environmental regulator for germination and macrocotyledon development in Streptocarpus rexii (Gesneriaceae) (2012)
  • Author(s): Nishii, K., Nagata, Toshiyuki, Wang, C.-N., Möller, M.
  • Journal Title: South African Journal of Botany Volume: Vol. 81 Pages: 50-60
  • Peer Reviewed
• [Journal Article] Gibberellin as a suppressor of lateral dominance and inducer of apical growth in the unifoliate Streptocarpus wendlandii (Gesneriaceae) (2012)
  • Author(s): Nishii, K., Wang, C.-N., Spada, A., Nagata, Toshiyuki, Möller, M.
  • Journal Title: New Zealand Journal of Botany Volume: Vol. 50 Pages: 1-21
  • Peer Reviewed
• [Journal Article] Metal ion homeostasis mediated by NRAMP transporters in plant cells : Focused on increased resistance to iron and cadmium ion (2012)
  • Author(s): Sano, T., Yoshihara, T., Handa, K. Sato, MH. Nagata, Toshiyuki and Hasezawa, S.
  • Journal Title: In Cross Talk and Membrane Trafficking Pathways, Roberto Weigert ed Volume: Chapter 10 Pages: 213-228
  • Peer Reviewed
• [Journal Article] Identification of amino acid residues essential for onion lachrymatory factor synthase activity (2012)
  • Author(s): Masamura, N., Ohashi, W., Tsuge, N., Imai, S., Ishii-Nakamura, A., Hirota, H., Nagata, Toshiyuki and Kumagai, N.
  • Journal Title: Bioscience, Biotechnology and Biochemistry Volume: Vol. 73 Pages: 447-453
  • Peer Reviewed
• [Journal Article] 小石川植物園-王立キュー植物園-ベルリン・ダーレム植物園をつなぐもの (2012)
  • Author(s): 長田敏行、東馬哲雄、邑田仁、Crane, P.
  • Journal Title: 遺伝 Volume: Vol. 66 Pages: 578-582
  • NAID: 40019432721
• [Journal Article] バイオマスの拡がり (2012)
  • Author(s): 長田敏行
  • Journal Title: 遺伝 Volume: Vol. 66 Pages: 79-81
• [Journal Article] ジャガイモゲノムの決定 (2012)
  • Author(s): 長田敏行
  • Journal Title: 遺伝 Volume: Vol. 66 Pages: 8-11
• [Journal Article] Gibberellin as a suppressor of lateral doinace and inducer of apical growth in the unifoliate Streptocarpus wendlandii (Gesneriaceae) (2012)
  • Author(s): K. Nishii, C.-N. Wang, A. Spada, T. Nagata, M. Moeller
  • Journal Title: New Zealand Journal of Botany Volume: 50 Issue: 3 Pages: 267-287
  • DOI: 10.1080/0028825x.2012.671775
  • Peer Reviewed
• [Journal Article] Light as environmental regulator for germination and macrocotyledon development in Streptocarpus rexii (Gesneriaceae) (2012)
  • Author(s): K. Nishii, T. Nagata, C.-N. Wang, M. Moeller
  • Journal Title: South African Journal of Botany Volume: 81 Pages: 50-60
  • DOI: 10.1016/j.sajb.2012.05.003
  • Peer Reviewed
• [Presentation] イチョウの生命誌 (2013)
  • Author(s): 長田敏行
  • Organizer: 日本メンデル協会公開講演会
  • Place of Presentation: 長野県下諏訪町
  • Year and Date: 2013-11-09
  • Invited
• [Presentation] サステイナビリテイにより覚醒された科学哲学への関心 (2013)
  • Author(s): 長田敏行
  • Organizer: 日本科学哲学会年会
  • Place of Presentation: 東京都千代田区市ヶ谷
  • Invited
• [Presentation] 小石川、キュー、ベルリン-ダーレム植物園をつなぐ「加藤竹斎」図版 (2013)
  • Author(s): 長田敏行、A. DuVal、東馬哲雄、邑田仁、P.R. Crane
  • Organizer: 日本植物園協会第48回大会
  • Place of Presentation: 茨城県つくば市
• [Presentation] 新奇細胞増殖因子NtXYL1の解析 (2013)
  • Author(s): 清水隆、長田敏行
  • Organizer: 日本植物生理学会大会
  • Place of Presentation: 岡山県岡山市
• [Presentation] 小石川・キュー・ベルリン-ダーレム植物園をつなぐ「加藤竹斎」図版 (2013)
  • Author(s): 長田敏行
  • Organizer: 日本植物園協会第48回大会
  • Place of Presentation: 国立科学博物館筑波実験植物園、茨城県つくば市
• [Presentation] サステイナビリテイにより覚醒された科学哲学への関心 (2013)
  • Author(s): 長田敏行
  • Organizer: 日本科学哲学会
  • Place of Presentation: 法政大学市ヶ谷校舎、東京都
  • Invited
• [Presentation] 新奇細胞増殖因子NtXYL1の解析 (2013)
  • Author(s): 清水隆、長田敏行
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学(岡山県)
• [Presentation] 1878年からの贈りもの (2012)
  • Author(s): 長田敏行、東馬哲雄、邑田仁、P.R. Crane
  • Organizer: 日本植物学会大会
  • Place of Presentation: 兵庫県姫路市
• [Presentation] A novel cell division factor that induced cell division in auxin-starved tobacco BY-2 cells (2012)
  • Author(s): T. Shimizu, T. Nagata
  • Organizer: The 3^<rd> EMBO Conference of Plant Molecular Biology
  • Place of Presentation: Matera, Italy
• [Presentation] イチョウの生命誌
  • Author(s): 長田敏行
  • Organizer: 日本メンデル協会公開講演会
  • Place of Presentation: 下諏訪町町民センター、長野県
  • Invited
• [Book] イチョウの自然誌と文化史 (2014)
  • Author(s): 長田敏行
  • Total Pages: 204
  • Publisher: 裳華房