| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Oil body is a plant organelle that exists in cells of fatty seed. It accumulates triacylglycerol. Aim of this research is to identify genes involved in biogenesis of the oil body, and to reveal function of the gene product. I established transgenic Arabidopsis expressing a fusion protein composed of oleosin, an oil body membrane protein, and GFP. This transgenic plant was used to develop live-imaging technique to visualize oil body in cells of living plant. Using this technique, seven mutants with aberrant morphology of oil body have been isolated. Of these, two mutants were precisely analyzed. It allowed identification of two genes responsible for these mutants and characterization of the gene products. Map-base cloning of genes responsible for the rest of mutants is under investigation.
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