Molecular genetical study on oil body biogenesis
Project/Area Number |
24657039
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Nagahama Institute of Bio-Science and Technology (2013-2014) National Institute for Basic Biology (2012) |
Principal Investigator |
HAYASHI Makoto 長浜バイオ大学, バイオサイエンス学部, 教授 (50212155)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 種子 / オルガネラ / オイルボディ / オレオシン / 植物油脂 / バイオ燃料 / バイオディーゼル / バイオマス |
Outline of Final Research Achievements |
Oil body is a plant organelle that exists in cells of fatty seed. It accumulates triacylglycerol. Aim of this research is to identify genes involved in biogenesis of the oil body, and to reveal function of the gene product. I established transgenic Arabidopsis expressing a fusion protein composed of oleosin, an oil body membrane protein, and GFP. This transgenic plant was used to develop live-imaging technique to visualize oil body in cells of living plant. Using this technique, seven mutants with aberrant morphology of oil body have been isolated. Of these, two mutants were precisely analyzed. It allowed identification of two genes responsible for these mutants and characterization of the gene products. Map-base cloning of genes responsible for the rest of mutants is under investigation.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis2015
Author(s)
Kazusato Oikawa, Shigeru Matsunaga, Shoji Mano, Maki Kondo, Kenji Yamada, Makoto Hayashi, Takatoshi Kagawa, Akeo Kadota, Wataru Sakamoto, Shoichi Higashi, Masakatsu Watanabe, Toshiaki Mitsui, Akinori Shigemasa, Takanori Iino, Yoichiroh Hosokawa & Mikio Nishimura
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Journal Title
Nature Plants
Volume: 1
Issue: 4
Pages: 15035-15035
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Highly Oxidized Peroxisomes Are Selectively Degraded via Autophagy in Arabidopsis2013
Author(s)
Shibata, M., Oikawa, K., Yoshimoto, K., Kondo., M., Mano, S., Yamada, K, Hayashi, M, Sakamoto, W., Ohsumi. Y., and Nishimura. M
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Journal Title
The Plant Cell
Volume: 25
Issue: 12
Pages: 4967-4983
DOI
Related Report
Peer Reviewed
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[Presentation] Loss of Arabidopsis 5’-3’ exoribonuclease AtXRN4 function enhances heat tolerance under short-time heat stress treatments2015
Author(s)
Nguyen, H. A., Matsui, A., Tanaka, M., Mizunashi, K., Nakaminami, K., Hayashi, M., Iida, K., Toyada, T., Nguyen, V. D., Seki, M.
Organizer
日本植物生理学会年会
Place of Presentation
東京農業大学
Year and Date
2015-03-16 – 2015-03-18
Related Report
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[Presentation] フェムト秒レーザーを用いた光依存的オルガネラ間接着力評価2014
Author(s)
及川和聡, 真野昌二, 林 誠, 山田 健志, 近藤 真紀, 東 正一, 渡辺 正勝, 三ツ井 敏明, 飯野 敬矩, 重政 彰徳, 細川 陽一郎, 西村 幹夫
Organizer
日本植物生理学会年会
Place of Presentation
富山
Related Report
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