| Project/Area Number |
24657041
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
SEKI Motoaki 独立行政法人理化学研究所, 環境資源科学研究センター, チームリーダー (80281624)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUI Akihiro 独立行政法人理化学研究所, 環境資源科学研究センター, 研究員 (90443027)
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| Project Period (FY) |
2012-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 植物分子機能 / small RNA / 植物 / ストレス / 発現制御 / 遺伝子 / 環境ストレス応答 / RNA制御 / アンチセンスRNA |
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| Research Abstract |
Arabidopsis transcriptome analysis under drought, cold, high-salinity stress and ABA treatment conditions, using tiling array, showed that approximately 6,000 novel transcripts existed in the antisense strand of the AGI code genes and they were induced under abiotic stress. To study the biogenesis mechanism of the antisense RNAs, accumulation of RD29A antisense RNA was analyzed, using RNA-dependent RNA polymerase (RDR) mutant series. Finally, we found that rdr1/2/6 mutation decreased accumulation of RD29A antisense RNA. RNase protection and RNA decay analyses showed that RD29A antisense RNA formed double stranded RNA and promoted degradation of RD29A mRNA. However, RNA-seq analysis did not detect siRNA accumulation in this locus. These results show that antisense RNAs are produced by RNA-dependent RNA polymerase (RDR) 1/2/6 without involving siRNA generation.
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