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Cell culture method for the study of secretory activation in mammary epithelial cells

Research Project

Project/Area Number 24657044
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionUniversity of Tsukuba

Principal Investigator

Matsuda Manabu  筑波大学, 医学医療系, 講師 (30282726)

Research Collaborator KASAI Hiroaki  
INOUE Naoya  
NEMOTO Tasuku  
OKAMURA Naomichi  
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordslactation / tissue culture / milkstasis / histamine / 乳腺 / 乳汁合成 / ヒスタミン / セロトニン / タイトジャンクション / 細胞極性 / 泌乳 / ミルク / リン酸輸送体 / 乳汁分泌 / 細胞培養 / 分泌活性化 / ヒスチジン脱炭酸酵素
Outline of Final Research Achievements

Mammary epithetial cells produce and secrete milk in response to a full set of appropriate systemic and local stimuli. Lack of one of the necessary signals attenuates full activation of lactation. Here, it has been revealed that local signals such as histamine/H1 receptor, in addition to systemic hormone such as insulin and cortisol, contribute to milkstasis via maintaining polarity of the mammary epithelial cells. Although all of these signals were found to be necessary for milk secretion in vivo, the best cocktail of these factors were not yet sufficient to make mammary epithetial cells produce abundant milk in vitro. Innovation of bioreactor to produce milk awaits further studies.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (11 results)

All 2015 2014 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (10 results)

  • [Journal Article] Activated vitamin D3 and pro-activated vitamin D3 attenuate induction of permanent changes caused by neonatal estrogen exposure in the mouse vagina2014

    • Author(s)
      Manabu Matsuda, Keiko Kurosaki, Naomichi Okamura
    • Journal Title

      The Journal of Reproduction and Development

      Volume: 60

    • NAID

      130003390842

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Involvement of histamine-signaling via histamine receptor type 1 in systemic hormone-induced integration of blood-milk barrier in bovine mammary epithetial cells2015

    • Author(s)
      松田 学
    • Organizer
      乳腺・泌乳研究会 第46回大会
    • Place of Presentation
      東京大学弥生キャンパス(東京都)
    • Year and Date
      2015-12-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] Effects of histamine receptor antagonists on the ion-permeability of bovine-, mouse- and human-derived mammary epithelial cell sheets2015

    • Author(s)
      根本 侑玖、松田 学
    • Organizer
      乳腺・泌乳研究会 第46回大会
    • Place of Presentation
      東京大学弥生キャンパス(東京都)
    • Year and Date
      2015-12-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒスタミンは複数の経路により乳腺上皮細胞のイオン透過性に影響する2015

    • Author(s)
      松田 学、根本 侑玖
    • Organizer
      日本動物学会 第86回大会
    • Place of Presentation
      朱鷺メッセ(新潟市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] マウス乳腺リン酸輸送体の発現2014

    • Author(s)
      松田学
    • Organizer
      乳腺・泌乳研究会第45回研究集会
    • Place of Presentation
      東京大学弥生キャンパス
    • Year and Date
      2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] マウス乳腺の分泌活性化に伴うリン酸輸送体の発現変化2014

    • Author(s)
      松田学,井上尚弥,岡村直道
    • Organizer
      日本動物学会大会
    • Place of Presentation
      東北大学
    • Year and Date
      2014-09-10 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] MCF-10A細胞におけるヒスタミンの作用経路2013

    • Author(s)
      井上尚哉,葛西裕亮,岡村直道,松田学
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 乳腺上皮細胞の透過性に対するヒスタミンシグナル阻害の影響2012

    • Author(s)
      松田 学
    • Organizer
      日本動物学会
    • Place of Presentation
      大阪大学(大阪府)
    • Related Report
      2012 Research-status Report
  • [Presentation] マウスおよびウシにおける乳腺上皮透過性に対するヒスタミンの作用2012

    • Author(s)
      松田 学
    • Organizer
      日本比較内分泌学会
    • Place of Presentation
      福井大学(福井県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 乳腺のヒスタミンシグナル阻害による密着結合関連遺伝子の発現変化

    • Author(s)
      松田学,葛西裕亮,井上尚哉
    • Organizer
      第66回日本動物学会関東支部大会
    • Place of Presentation
      東京大学柏キャンパス(千葉県)
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒト乳腺上皮由来MCF10A細胞の上皮膜透過性に対するヒスチジン受容体阻害剤の影響

    • Author(s)
      松田 学
    • Organizer
      日本動物学会関東支部大会
    • Place of Presentation
      東京工業大学(東京都)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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