| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The dioecious plant species, Silene latifolia, has a sex determination mechanism based on an active Y chromosome. We used inter-specific hybrids (Correns’ cross, 1928) in the genus Silene to study the effects of gene complexes on the Y chromosome. If the function of Y-linked genes has been maintained in the same state as in the hermaphrodite progenitor species, it should be possible to substitute such genes by genes coming from the related hermaphrodite species, S. viscosa. In the inter-specific hybrid, S. latifolia x S. viscosa, anthers indeed develop far beyond the early bilobal stage characteristic of XX in S. latifolia female plants. We also examined a detailed comparative histological comparison of anther development in hybrids and in wild-type males and Y-deletion mutants generated by heavy-ion beam irradiation. These mutant phenotypes were classified into two groups: a hermaphrodite group and an asexual group with defects in their gynoecium and/or stamen.
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