An investigation into the plant dioecism by Correns' cross and the high throughput 4D screening
Project/Area Number |
24657046
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Morphology/Structure
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Research Institution | The University of Tokyo |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | SlAP3 / 欠失 / 性染色体 / 重イオンビーム / 雌雄異株植物 / ヒロハノマンテマ / 雌雄同株 / コレンス交雑 / 雌雄異株 |
Outline of Final Research Achievements |
The dioecious plant species, Silene latifolia, has a sex determination mechanism based on an active Y chromosome. We used inter-specific hybrids (Correns’ cross, 1928) in the genus Silene to study the effects of gene complexes on the Y chromosome. If the function of Y-linked genes has been maintained in the same state as in the hermaphrodite progenitor species, it should be possible to substitute such genes by genes coming from the related hermaphrodite species, S. viscosa. In the inter-specific hybrid, S. latifolia x S. viscosa, anthers indeed develop far beyond the early bilobal stage characteristic of XX in S. latifolia female plants. We also examined a detailed comparative histological comparison of anther development in hybrids and in wild-type males and Y-deletion mutants generated by heavy-ion beam irradiation. These mutant phenotypes were classified into two groups: a hermaphrodite group and an asexual group with defects in their gynoecium and/or stamen.
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Report
(4 results)
Research Products
(101 results)
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[Journal Article] Mutation rates of Parachlorella kessleri by heavy ion beam irradiation and classification of their genomic deletion types.2014
Author(s)
Yamazaki, T., Ota, S., Oshima, K. Hattori, M., Kazama, Y., Abe, T. and Kawano, S.
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Journal Title
RIKEN Accel. Prog. Rep.
Volume: 47
Pages: 301-301
Related Report
Peer Reviewed
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[Journal Article] HAP2/GCS1 Is Involved in the Sexual Reproduction System of the Marine Macroalga<i> Ulva compressa</i> (Ulvales, Chlorophyta)2014
Author(s)
Yamazaki, T., Endo, M., Ito, K., Suzuki, R., Ota, S., Kuwano, K., Miyamura, S., Toyoda, A. and Kawano, S.
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Journal Title
CYTOLOGIA
Volume: 79
Issue: 4
Pages: 575-584
DOI
NAID
ISSN
0011-4545, 1348-7019
Related Report
Peer Reviewed / Open Access
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[Journal Article] Use of FM1-43, a membrane-specific fluorescent dye, to estimate plasma membrane integrity in the cryopreservation of green algae.2014
Author(s)
Yamazaki, T., Hirai, C., Ota, S., Kuwano, K., and Kawano, S.
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Journal Title
Cryo Letters
Volume: 35
Pages: 180-187
Related Report
Peer Reviewed
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[Journal Article] Starch and lipid accumulation in eight strains of six Chlorella species under strong illumination and aeration culture conditions.2014
Author(s)
Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., and Kawano, S.
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Journal Title
Bioresour. Technol.
Volume: 158
Pages: 127-134
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Rapid degeneration of noncoding DNA regions surrounding SlAP3X/Y after recombination suppression in a dioecious plant, Silene latifolia.2013
Author(s)
Ishii, K., Nishiyama, R., Shibata, F., Kazama, Y., Abe, T., and Kawano, S.
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Journal Title
G3
Volume: 3
Issue: 12
Pages: 2121-2130
DOI
Related Report
Peer Reviewed
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[Journal Article] Phenotypic spectrum of Parachlorella kessleri (Chlorophyta) mutants produced by heavy-ion irradiation.2013
Author(s)
Ota, S., Matsuda, T., Takeshita, T., Yamazaki, T., Kazama, Y., Abe, T., Kawano, S.
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Journal Title
Bioresour. Technol.
Volume: 149
Pages: 432-438
DOI
Related Report
Peer Reviewed
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[Journal Article] Relationship between starch and lipid accumulation induced by nutrient depletion and replenishment in the microalga Parachlorella kessleri.2013
Author(s)
Fernandes, B., Teixeira, J., Dragone, G., Vicente, A.A., Kawano, S., Bisova, K., Pribyl, P., Zachleder, V., and Vitova, M.
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Journal Title
Bioresour. Technol.
Volume: 144
Pages: 268-274
DOI
Related Report
Peer Reviewed
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[Journal Article] Single-membrane-bounded peroxisome division revealed by isolation of dynamin-based machinery2013
Author(s)
Yuuta Imoto, Haruko Kuroiwa, Yamato Yoshida, Mio Ohnuma, Takayuki Fujiwara, Masaki Yoshida, Keiji Nishida, Fumi Yagisawa, Shunsuke Hirooka, Shinya Miyagishima, Osami Misumi, Shigeyuki Kawano, Tsuneyoshi Kuroiwa
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 110
Issue: 23
Pages: 9583-9588
DOI
Related Report
Peer Reviewed
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[Journal Article] Localization and evolution of septins in algae2013
Author(s)
Yamazaki, T., Owari, S., Ota, S., Sumiya, N., Yamamoto, M., Watanabe, K., Nagumo, T., Miyamura, S. and Kawano, S
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Journal Title
Plant J
Volume: (in press)
Issue: 4
Pages: 12147-8
DOI
Related Report
Peer Reviewed
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[Journal Article] Three dimensional ultrastructural study of oil and astaxanthin accumulation during encystment in the green alga Haematococcus pluvialis2013
Author(s)
Wayama, M., Ota, O., Matsuura, M., Nango, N., Hirata, A., and Kawano, S
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Journal Title
PLoS ONE
Volume: 8(1)
Issue: 1
Pages: 53618-9
DOI
Related Report
Peer Reviewed
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[Journal Article] Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species.2013
Author(s)
Mizuno, Y., Sato, A., Watanabe, K., Hirata, A., Takeshita, T., Ota, S., Sato, N., Zachleder, V., Tsuzuki, M., and Kawano, S.
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Journal Title
Bioresour. Technol.
Volume: 129
Pages: 150-155
Related Report
Peer Reviewed
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[Journal Article] The microalga Parachlorella kessleri: A novel highly efficient lipid producer.2013
Author(s)
Li, X., Pribyl, Binová K., Kawano, S., Cepak, V., Zachleder, V., Cizkova, M., Branyikova, I., and Vítová, M.
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Journal Title
Biotechnol. Bioeng.
Volume: 110
Issue: 1
Pages: 97-107
DOI
Related Report
Peer Reviewed
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[Journal Article] Imbalanced functional-disorders between pollen germination and seed formation by heavy-ion beam and γ-ray irradiation.2013
Author(s)
Aonuma, W., Ishii, K., Fujita, N., Shimizu, Y., Kazama, Y., Abe, T., and Kawano, S.
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Journal Title
RIKEN Accel. Prog. Rep.
Volume: 45
Pages: 217-217
Related Report
Peer Reviewed
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[Journal Article] Isolation of mitochondrial and plastid ftsZ genes and analysis 1 of the organelle targeting sequence in the diatom Chaetoceros neogracile (Diatoms,Bacillariophyceae)2012
Author(s)
Nishikawa, T., Moriyama, Y., Sato, M., Sano, T., Hasezawa, S., Ota, S., and Kawano, S
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Journal Title
Phycol. Res
Volume: 60
Issue: 2
Pages: 123-136
DOI
Related Report
Peer Reviewed
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[Journal Article] Chloroplast Division and Differentially Regulated Expression of <i>FtsZ1</i> and <i>FtsZ2</i> in the Synchronous Culture of <i>Nannochloris bacillaris</i> (Chlorophyta, Trebouxiophyceae)2012
Author(s)
Sumiya, N., Watanabe, K., Owari, S., Yamamot, M., and Kawano, S.
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Journal Title
CYTOLOGIA
Volume: 77
Issue: 1
Pages: 59-66
DOI
NAID
ISSN
0011-4545, 1348-7019
Related Report
Peer Reviewed
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[Journal Article] A petal-less flower caused by a Microbotryum violaceum mutant2011
Author(s)
Fujita, N., Aonuma, W., Shimizu, Y., Yamanaka, K., Hirata, A., Hood, M.E., Kawano, S.
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Journal Title
Int.J.Plant Sci
Volume: 173
Issue: 5
Pages: 1-11
DOI
Related Report
Peer Reviewed
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[Journal Article] Narrowing Down the Mapping of Plant Sex-Determination Regions Using New Y Chromosome-Specific Markers and Heavy-Ion-Beam Irradiation-Induced Y Deletion Mutants in Silene latifolia2011
Author(s)
Fujita, N., Torii, C., Ishii, K., Aonuma, W., Shimizu, Y., Kazama, Y., Abe, T., Kawano, S.
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Journal Title
G3
Volume: 2
Issue: 2
Pages: 271-278
DOI
Related Report
Peer Reviewed
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[Presentation] 藻類の雌雄非対称性の成立2014
Author(s)
河野重行
Organizer
ゲノム支援公開シンポジウム
Place of Presentation
東京国際フォーラム(東京都、千代田区)
Year and Date
2014-12-12
Related Report
Invited
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[Presentation] 藻類バイオと電顕3D2014
Author(s)
大田修平、河野重行
Organizer
日本植物学会第78回大会
Place of Presentation
明治大学 生田キャンパス(神奈川県、川崎市)
Year and Date
2014-09-13
Related Report
Invited
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[Presentation] Phenotypic spectrum and 3D/TEM analysis of Parachlorella kessleri mutants produced by heavy-ion irradiation.2014
Author(s)
Ota, S., Takeshita, T., Yamazaki, T., Vítová, M., Zachleder, V., Abe, T., Kawano, S.
Organizer
BioTech2014 and 6th Czech-Swiss Symposium
Place of Presentation
Prague (Czech Republic)
Year and Date
2014-06-11 – 2014-06-14
Related Report
Invited
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[Presentation] Starch and lipid accumulation in Chlorella species under high light intensity in the laboratory and outdoor mass cultures.2014
Author(s)
Takeshita, T., Ivanov, V., Ota, S., Yamazaki, T., Zachleder, V., Kawano, S.
Organizer
BioTech2014 and 6th Czech-Swiss Symposium
Place of Presentation
Prague (Czech Republic)
Year and Date
2014-06-11 – 2014-06-14
Related Report
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[Presentation] Allo-authentication revealed by sexual asymmetry and uniparental organelle-inheritance of male- and femalegametes in the green alga, Ulva compressa.2012
Author(s)
Suzuki, R., Endo, M., Mogi, Y., Miyamura, S., Kuwano, K. and Kawano, S.
Organizer
International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants. Joint Meeting of the 2nd Allo-authentication Meeting and the 5th Egg-Coat Meeting (MCBEEC)
Place of Presentation
Hotel Nagoya Garden Palace, Nagoya, Japan
Related Report
Invited
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[Presentation] Division behavior of mitochondrion and microbody in the red algae Cyanidioschyzon merolae.2012
Author(s)
Imoto, Y., Ohnuma, M., Yoshida, Y., Kuroiwa, H., Kuroiwa, T., and Kawano. S.
Organizer
10th International PhD Student Conference on Experimental Plant Biology
Place of Presentation
Brno, Czech Republic
Related Report
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[Presentation] Three-dimensional ultra-structural study of encystment and astaxanthin accumulation in the green alga, Haematococcus pluvialis.2012
Author(s)
Ota, S., Wayama, M., Nango, N., Hirata, A. and Kawano, S.
Organizer
Protist 2012 ( 19th ISEP and 62nd ISOP joint meeting)
Place of Presentation
Oslo, Norway
Related Report
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[Book] Atlas of Plant cell Structure, Springer2014
Author(s)
Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hyashi, Y., (eds)
Publisher
Springer
Related Report
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