Analysis of a candidate protein complex for the receptor of the maturation-inducing hormone of starfish oocytes

• Project/Area Number: 24657047
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Morphology/Structure
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: OKUMURA Eiichi 東京工業大学, 生命理工学研究科, 助教 (00323808)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ヒトデ / 卵成熟 / ホルモン / 受容体 / GPCR / ナノアフィニティービーズ / 1-メチルアデニン / シグナル伝達

Research Abstract

Maturation-inducing hormone (MIH) induces maturation of animal oocytes arrested at pro-phase of first meiosis. Starfish MIH is 1-methyladenine (1-MeAde), which is first identified MIH in all animal. Even though there are attempts on identification of the receptor of 1-MeAde, it is only revealed as a G-protein coupled receptor (GPCR) and its molecule is still unclear. I tried the receptor identification using a novel material, high-performance affinity beads (FG-beads or Handa beads). Three proteins are identified as binding proteins. These proteins are coded by a cDNA homologous to the rendezvin gene. Starfish rendezvin has two furin protease sites and furin can process them. Since starfish rendezvin has no 7 transmembrane domain of GPCR, it might bind to some GPCR. A candidate GPCR is cloned for the binding partner of starfish rendezvin. A partial fragment of it binds to rendezvin. I propose 1-methyladenine receptor as a unique protein complex of rendezvin and a novel GPCR.

Research Products

• [Journal Article] Cyclin B–Cdk1 inhibits protein phosphatase PP2A-B55 via a Greatwall kinase–independent mechanism (2014)
  • Author(s): Okumura, E., Morita, A., Wakai, M., Mochida, S., Hara, M. & Kishimoto, T.
  • Journal Title: The Journal of cell biology Volume: 204 Pages: 881-889
• [Journal Article] Cyclin B-Cdk1 inhibits protein phosphatase PP2A-B55 via a Greatwall kinase-independent mechanism. (2014)
  • Author(s): Okumura, E., Morita, A., Wakai, M., Mochida, S., Hara, M., and Kishimoto, T.
  • Journal Title: J. Cell Biol. Volume: 204 Issue: 6 Pages: 881-889
  • DOI: 10.1083/jcb.201307160
  • Peer Reviewed
• [Journal Article] Antibody inhibition of protein activity in starfish oocytes. (2014)
  • Author(s): Okumura E, Hara M, Kishimoto T.
  • Journal Title: Methods Mol Biol. Volume: 1128 Pages: 311-330
  • DOI: 10.1007/978-1-62703-974-1_21
  • ISBN: 9781627039734, 9781627039741
• [Journal Article] Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase promoting factor. (2012)
  • Author(s): Hara, M., …., Kishimoto, T.
  • Journal Title: Nat. Commun. Volume: 3 Issue: 1 Pages: 1059-1059
  • DOI: 10.1038/ncomms2062
  • Peer Reviewed
• [Presentation] ヒトデ卵減数分裂再開時におけるPP2A阻害因子Ensa/ARPP19のCdc2による制御 (2012)
  • Author(s): 奥村英一、森田敦、持田悟、原昌稔、岸本健雄
  • Organizer: 分子生物学会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Book] Antibody inhibition of protein activity in starfish oocytes. Methods in Mol. Biol. 1128 (2014)
  • Author(s): Okumura, E., Hara, M. & Kishimoto, T.