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Novel approaches to insect learning systems using transgenic crickets

Research Project

Project/Area Number 24657049
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Animal physiology/Animal behavior
Research InstitutionHokkaido University

Principal Investigator

MIZUNAMI Makoto  北海道大学, 理学(系)研究科(研究院), 教授 (30174030)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsコオロギ / 学習 / 遺伝子組換え / RNA干渉 / 記憶 / 遺伝子発現阻害 / ドーパミン / オクトパミン / ドーパミン受容体 / 一酸化窒素合成酵素
Research Abstract

We attempted to establish (1) transgenic techniques and (2) RNA interference (RNAi) methods to study molecular basis of learning in crickets, Gryllus bimaculatus. For the former, we successfully obtained heterozygous knockout individuals of type 1 dopamine receptor (DopR1) by using CRISPR/CAS system, and now we are now trying to obtain homozygous DopR1 knockout crickets by inbreeding them. For the latter, we performed RNAi experiments with to types of dopamine receptors (Dop1 and Dop2) and a type of octopamine receptor (OA1) and obtained evidence to show that DopR1 participates in aversive learning and OA1 participates in appetitive condoning, whereas DA2 participates in neither of them. The results provided convincing evidence that octopamine neurons and dopamine neurons mediate reward and punishment signals, respectively, in associative learning in crickets.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (59 results)

All 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (36 results) (of which Invited: 8 results) Book (8 results) Remarks (8 results)

  • [Journal Article] Cyclic nucleotide–gated channels, calmodulin, adenylyl cyclase and calcium/calmodulin-dependent kinase II are required for late but not early long-term memory formation in the honey bee2014

    • Author(s)
      Matsumoto Y, Sandoz JC, Devaud JM, Lormant F, Mizunami M, Giurfa M
    • Journal Title

      Learning & Memory

      Volume: 21 Issue: 5 Pages: 272-286

    • DOI

      10.1101/lm.032037.113

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Roles of NO signaling in long-term memory formation in visual learning in an insect.2013

    • Author(s)
      Matsumoto Y, Hirashima D, Terao K, Mizunami M.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 7 Pages: e68538-e68538

    • DOI

      10.1371/journal.pone.0068538

    • NAID

      120005342541

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis and modeling of neural processes underlying sensory preconditioning2013

    • Author(s)
      Matsumoto Y, Hirashima D, Mizunami M.
    • Journal Title

      Neurobiol. Learn. Mem.

      Volume: 101 Pages: 103-113

    • DOI

      10.1016/j.nlm.2013.01.008

    • NAID

      120005228719

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Participation of NO signaling in formation of long-term memory in salivary conditioning of the cockroach.2013

    • Author(s)
      Matsumoto, C.S., Kuramochi, T., Matsumoto, Y., Watanabe, H., Nishino, H., Mizunami, M.
    • Journal Title

      Neuroscience Letters

      Volume: 印刷中 Pages: 4-8

    • DOI

      10.1016/j.neulet.2013.01.010

    • NAID

      120005295655

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Divergent and convergent projections to the two parallel olfactory centers from two neighboring, pheromone-receptive glomeruli in the male American cockroach.2012

    • Author(s)
      Nishino H., Iwasaki M., Kamimura I., Mizunami M.
    • Journal Title

      Journal of Comparative Neurology

      Volume: 印刷中 Issue: 15 Pages: 3428-3445

    • DOI

      10.1002/cne.23111

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Visual and olfactory input segregation in the mushroom body calyces in a basal neopteran, the American cockroach2012

    • Author(s)
      Nishino H, Iwasaki M, Yasuyama K, Hongo H, Watanabe H, Mizunami M.
    • Journal Title

      Arthropod Structure & Development

      Volume: 41 Issue: 1 Pages: 3-16

    • DOI

      10.1016/j.asd.2011.08.005

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Context-dependent olfactory learning monitored by activities of salivary neurons in cockroaches.2012

    • Author(s)
      Matsumoto C., Matsumoto Y., Watanabe H., Nishino H., Mizunami M.
    • Journal Title

      Neurobiology of Learning and Memory

      Volume: 97 Issue: 1 Pages: 30-36

    • DOI

      10.1016/j.nlm.2011.08.010

    • NAID

      120003762641

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Intelligence with microbrains : Cognitive processes underlying insect learning2014

    • Author(s)
      Mizunami M
    • Organizer
      International workshop on animal instinctive and intelligent behaviors
    • Place of Presentation
      Hokkaido Univ. (Sapporo)
    • Year and Date
      2014-02-20
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] コオロギの古典的条件づけにおけるブロッキング2013

    • Author(s)
      寺尾勘太,松本幸久、水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2013-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] コオロギ古典的条件付けにおける習慣形成とオクトパミンニューロンの関係の解明2013

    • Author(s)
      廣鰭翔,松本幸久,水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山市
    • Year and Date
      2013-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] RNA干渉法によるコオロギの学習・記憶の分子メカニズムの解析2013

    • Author(s)
      和久田亮,中村太郎,松本幸久,野地澄晴,三戸太郎,水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2013-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] フタホシコオロギの加齢性記憶障害における飼育環境の影響2013

    • Author(s)
      松本幸久,松本-佐藤千尋,中野真樹,服部淳彦,水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2013-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] MERを用いたゴキブリの学習2013

    • Author(s)
      細野翔平,松本幸久,水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2013-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] コオロギにおけるブロッキング現象の確認2013

    • Author(s)
      寺尾勘太,松本幸久,水波誠
    • Organizer
      日本動物学会北海道支部会
    • Place of Presentation
      北海道教育大学(札幌市)
    • Year and Date
      2013-08-24
    • Related Report
      2013 Final Research Report
  • [Presentation] ゴキブリを用いた新しい学習実験系の確立2013

    • Author(s)
      細野翔平,松本幸久,水波誠
    • Organizer
      日本動物学会北海道支部会
    • Place of Presentation
      北海道教育大学(札幌市)
    • Year and Date
      2013-08-24
    • Related Report
      2013 Final Research Report
  • [Presentation] RNAiによるコオロギの記憶形成に関わる分子機構の解明2013

    • Author(s)
      和久田亮,松本幸久,水波誠
    • Organizer
      日本動物学会北海道支部会
    • Place of Presentation
      北海道教育大学(札幌市)
    • Year and Date
      2013-08-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis and modeling of sensory preconditioning in crickets2013

    • Author(s)
      Mizunami M, Matsumoto Y, Hirashima D
    • Organizer
      日本比較生理生化学会
    • Place of Presentation
      イーグレひめじ(姫路市)
    • Year and Date
      2013-07-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Memory molecules in insects : from behavioral pharmacological studies of olfactory learning in crickets. Symposium : Potential of insect neuroscience2012

    • Author(s)
      Matsumoto Y, Matsumoto C, Mizunami M
    • Organizer
      Japanese Society for Neuroscience, 日本神経科学学会
    • Place of Presentation
      名古屋コンベンションセンター(名古屋市)
    • Year and Date
      2012-09-20
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] コオロギ古典的条件付けにおける習慣形成の行動学的証明2012

    • Author(s)
      廣鰭翔、松本幸久、水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      大阪大学(大阪市)
    • Year and Date
      2012-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] フタホシコオロギの嗅覚学習におけるPKCシグナルの役割解析2012

    • Author(s)
      川中子沙織、松本幸久、水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      大阪大学(大阪市)
    • Year and Date
      2012-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] コオロギ古典的条件付けにおける習慣形成2012

    • Author(s)
      廣鰭翔、松本幸久、水波誠
    • Organizer
      日本動物学会北海道支部会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-08-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis and modeling of sensory preconditioning in crickets2012

    • Author(s)
      Mizunami M, Matsumoto Y, Hirashima D
    • Organizer
      10th International Congress of Neuroethology, Maryland
    • Place of Presentation
      Univ. Maryland (USA)
    • Year and Date
      2012-07-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Roles of aminergic neurons in memory acquisition and retrieval in crickets2012

    • Author(s)
      Mizunami M
    • Organizer
      International Symposium : Biogenic Amines in Insects
    • Place of Presentation
      Berlin Free University (Germany)
    • Year and Date
      2012-07-07
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Olfactory conditioning of mandible elevation response in cricket2012

    • Author(s)
      Matsumoto Y, Matsumoto C, Mizunami M
    • Organizer
      日本比較生理生化学会
    • Place of Presentation
      総合研究大学院大学(葉山市)
    • Year and Date
      2012-07-07
    • Related Report
      2013 Final Research Report
  • [Presentation] NO-cGMP signaling is involved in age-related memory impairment in the cricket Gryllus bimaculatus2012

    • Author(s)
      Matsumoto Y, Matsumoto C, Mizunami M
    • Organizer
      Second International Conference on the Cricket
    • Place of Presentation
      Tokushima Univ. (Tokushima)
    • Year and Date
      2012-03-21
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Systemic RNA interference for the study of long-term memory formation in the cricket2012

    • Author(s)
      Mizunami M, Takahashi T, Hamada A, Miyawaki K, Matsumoto Y, Mito T, Noji S
    • Organizer
      Second International Conference on the Cricket
    • Place of Presentation
      Tokushima Univ. (Tokushima)
    • Year and Date
      2012-03-19
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Analysis and modeling of sensory preconditioning in crickets

    • Author(s)
      Mizunami M, Matsumoto Y, Hirashima D
    • Organizer
      日本比較生理生化学会
    • Place of Presentation
      イーグレひめじ(姫路市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] コオロギにおけるブロッキング現象の確認

    • Author(s)
      寺尾勘太,松本幸久,水波誠
    • Organizer
      日本動物学会北海道支部会
    • Place of Presentation
      北海道教育大学(札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ゴキブリを用いた新しい学習実験系の確立

    • Author(s)
      細野翔平,松本幸久,水波誠
    • Organizer
      日本動物学会北海道支部会
    • Place of Presentation
      北海道教育大学(札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] コオロギの古典的条件づけにおけるブロッキング

    • Author(s)
      和久田亮,松本幸久,水波誠
    • Organizer
      日本動物学会北海道支部会
    • Place of Presentation
      北海道教育大学(札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] コオロギの古典的条件づけにおけるブロッキング

    • Author(s)
      寺尾勘太,松本幸久、水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] コオロギ古典的条件付けにおける習慣形成とオクトパミンニューロンの関係の解明

    • Author(s)
      廣鰭翔,松本幸久,水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] RNA 干渉法によるコオロギの学習・記憶の分子メカニズムの解析

    • Author(s)
      和久田亮,中村太郎,松本幸久,野地澄晴,三戸太郎,水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] フタホシコオロギの加齢性記憶障害における飼育環境の影響

    • Author(s)
      松本幸久,松本-佐藤千尋,中野真樹,服部淳彦,水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] MER を用いたゴキブリの学習

    • Author(s)
      細野翔平,松本幸久,水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Intelligence with microbrains: Cognitive processes underlying insect learning.

    • Author(s)
      Mizunami M
    • Organizer
      International workshop on animal instinctive and intelligent behaviors
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Olfactory conditioning of mandible elevation response in crickets

    • Author(s)
      Matsumoto Y, Matsumoto C, Mizunami M.
    • Organizer
      日本比較生理生化学会
    • Place of Presentation
      総合研究大学院大学(葉山市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Roles of aminergic neurons in memory acquisition and retrieval in crickets

    • Author(s)
      Mizunami M.
    • Organizer
      International Symposium: Biogenic Amines in Insects
    • Place of Presentation
      Frie Universitate Berlin (Germany)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Analysis and modeling of sensory preconditioning in crickets.

    • Author(s)
      Mizunami M, Matsumoto Y, Hirashima D
    • Organizer
      10th International Congress of Neuroethology
    • Place of Presentation
      University of Maryland (USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] コオロギ古典的条件付けにおける習慣形成

    • Author(s)
      廣鰭翔、松本幸久、水波誠
    • Organizer
      日本動物学会北海道支部会
    • Place of Presentation
      北海道大学( 札幌市)
    • Related Report
      2012 Research-status Report
  • [Presentation] コオロギ古典的条件付けにおける習慣形成の行動学的証明

    • Author(s)
      廣鰭翔、松本幸久、水波誠、
    • Organizer
      日本動物学会
    • Place of Presentation
      大阪大学( 吹田市)
    • Related Report
      2012 Research-status Report
  • [Presentation] フタホシコオロギの嗅覚学習におけるPKCシグナルの役割解析

    • Author(s)
      川中子沙織、松本幸久、水波誠
    • Organizer
      日本動物学会
    • Place of Presentation
      大阪大学( 吹田市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Memory molecules in insects: from behavioral pharmacological studies of olfactory learning in crickets

    • Author(s)
      Matsumoto Y, Matsumoto C, Mizunami M.
    • Organizer
      Japanese Society for Neuroscience
    • Place of Presentation
      名古屋コンベンションセンター( 名古屋市)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 行動生物学辞典(上田恵介他編、自己受容器などの項目や付録1を担当)2013

    • Author(s)
      水波誠
    • Publisher
      東京化学同人
    • Related Report
      2013 Final Research Report
  • [Book] 微小脳と巨大脳(項目担当)、高校生物教師用指導書(DVD版)2013

    • Author(s)
      水波誠
    • Publisher
      東京書籍
    • Related Report
      2013 Final Research Report
  • [Book] Chapter 41: Olfactory and visual learning in cockroaches and crickets. In : Invertebrate Learning and Memory, eds by R. Menzel and P.R.Benjamin2013

    • Author(s)
      Mizunami M., Matsumoto Y., Watanabe H., Nishino H.
    • Publisher
      Springer
    • Related Report
      2013 Final Research Report
  • [Book] 行動生物学辞典2013

    • Author(s)
      水波誠
    • Total Pages
      617
    • Publisher
      東京化学同人
    • Related Report
      2013 Annual Research Report
  • [Book] Invertebrate Learning and Memory2013

    • Author(s)
      Mizunami M., Matsumoto Y., Watanabe H., Nishino H.
    • Total Pages
      12
    • Publisher
      Springer-Verlag
    • Related Report
      2012 Research-status Report
  • [Book] 高校生物教師用指導用教材(DVD版)2013

    • Author(s)
      水波誠
    • Total Pages
      5
    • Publisher
      東京書籍
    • Related Report
      2012 Research-status Report
  • [Book] マダガスカルゴキブリの飼育(分担執筆)、針山孝彦他編、研究者が教える動物飼育第2巻「昆虫とクモの仲間」2012

    • Author(s)
      松本幸久、水波誠
    • Publisher
      共立出版
    • Related Report
      2013 Final Research Report
  • [Book] 研究者が教える動物飼育第2巻「昆虫とクモの仲間」2012

    • Author(s)
      松本幸久、水波誠
    • Total Pages
      4
    • Publisher
      共立出版
    • Related Report
      2012 Research-status Report
  • [Remarks] ①朝日新聞グローブ 「脳の不思議、鳥や虫の脳もすごい!」 2014年3月16日(取材協力) 報道関係情報

    • Related Report
      2013 Final Research Report
  • [Remarks] ②朝日新聞 「トンボの目玉はどうなっている?」 2012年6月9日(取材協力) 報道関係情報

    • Related Report
      2013 Final Research Report
  • [Remarks] ①北海道大学オープンキャンパス 高校生限定プログラム「昆虫の学習を体験しよう!」 2013年8月5日 北海道大学(札幌市) アウトリーチ活動

    • Related Report
      2013 Final Research Report
  • [Remarks] 日本生物教育会全国大会実験研修「"脳"をみんなの教材に」講師 2012年8月4日 北海道大学(札幌市) アウトリーチ活動

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      https://www.sci.hokudai.ac.jp/~mizunami/MICROB~2/

    • Related Report
      2013 Final Research Report
  • [Remarks] 水波誠研究室

    • URL

      https://www.sci.hokudai.ac.jp/~mizunami/MICROB~2/

    • Related Report
      2013 Annual Research Report
  • [Remarks] ようこそ水波研究室へ

    • URL

      http://www.sci.hokudai.ac.jp/~mizunami/MICROB~2/index.html

    • Related Report
      2012 Research-status Report
  • [Remarks] Invertebrate Brain Gallery

    • URL

      http://invbrain.neuroinf.jp/modules/newdb1/list.php?id=2&n=20&sort=1&sort_method=desc&item=0&ml_lang=en

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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