Toward the standard methodologies for next-generation molecular phylogenetics
| Project/Area Number |
24657065
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | National Museum of Nature and Science, Tokyo |
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Principal Investigator |
OKUYAMA YUDAI 独立行政法人国立科学博物館, 植物研究部, 研究員 (40522529)
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| Co-Investigator(Kenkyū-buntansha) |
TANABE Akifumi 独立行政法人水産総合研究センター, その他部局等, 研究員 (40549044)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 分子系統樹 / 次世代シーケンサー / RAD-seq / テルペン合成酵素遺伝子 / チャルメルソウ / カンアオイ / RADタグ法 |
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| Research Abstract |
This study aimed to establish the standard methodologies for phylogenetic inferences on the plant groups that underwent rapid species radiation. To this end, two next-generation-sequencer-based methodologies were examined, i.e., RAD-seq and RNA-seq. In the earlier trial of the RAD-seq, we found that the amount of the data output by GS Junior is far from that necessary for consistent phylogenetic study, so we switched to the use of Hiseq 2000. Now we have finished the library preparation and the data processing is underway. As for the RNA-seq, we analyzed the flower-expressed genes and succeeded to find some genes with unusual mode of molecular evolution, possibly because of their association with the plant speciation process. The gene regions were found to have much more information than the standard genes used for the routine phylogenetic studies.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding.2013
Author(s)
Stella Cesari, Gaetan Thilliez, Cecile Ribot, Veronique Chalvon, Corinne Michel, Alain Jauneau, Susana Rivas, Ludovic Alaux, Hiroyuki Kanzaki, Yudai Okuyama, Jean-Benoit Morel, Elisabeth Fournier, Didier Tharreau, Ryohei Terauchi, Thomas Kroj
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Journal Title
The Plant Cell.
Volume: 25
Pages: 1463-1481
Related Report
Peer Reviewed
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