Development of a high-pressure freeze trap method of protein crystals

Research Project

Project/Area Number	24657071
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Structural biochemistry
Research Institution	Nagoya University
Principal Investigator	WATANABE Nobuhisa 名古屋大学, シンクロトロン光研究センター, 教授 (70212321)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	高圧結晶構造解析 / ダイヤモンドアンビルセル
Research Abstract	X-ray radiation damage is a serious problem for the high-pressure protein crystallography, because the method uses a diamond anvil cell and it is necessary to perform experiments at room temperature. In this study, we have tried to develop a new method that makes protein crystals frozen under high-pressure condition. A new apparatus has been developed. However, we found the mother liquid oozes from the crystal when we pressurize protein crystals, and that degrades crystal quality during the freezing process. We could not find a workaround for this problem.

Report

(3 results)