Development of a high-pressure freeze trap method of protein crystals
| Project/Area Number |
24657071
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
WATANABE Nobuhisa 名古屋大学, シンクロトロン光研究センター, 教授 (70212321)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 高圧結晶構造解析 / ダイヤモンドアンビルセル |
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| Research Abstract |
X-ray radiation damage is a serious problem for the high-pressure protein crystallography, because the method uses a diamond anvil cell and it is necessary to perform experiments at room temperature. In this study, we have tried to develop a new method that makes protein crystals frozen under high-pressure condition. A new apparatus has been developed. However, we found the mother liquid oozes from the crystal when we pressurize protein crystals, and that degrades crystal quality during the freezing process. We could not find a workaround for this problem.
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Report
(3 results)
Research Products
(4 results)
