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Purification of mega-complexes involved in preprotein translocation across the chloroplast envelope membranes

Research Project

Project/Area Number 24657073
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

NAKAI Masato  大阪大学, たんぱく質研究所, 准教授 (90222158)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords蛋白質膜透過 / 細胞内蛋白質輸送 / オルガネラ / 葉緑体 / 膜蛋白質複合体 / プラスチド / 細胞内タンパク質輸送 / タンパク質膜透過 / 植物 / 内共生 / 輸送モーター / タンパク質膜透過装置 / タンパク質輸送 / オルガネラ形成 / 生合成 / タンパク質立体構造解析 / 電子顕微鏡観察 / トランスロコン / 蛋白質輸送 / 端粒子解析 / 形質転換植物
Outline of Final Research Achievements

Elucidation of molecular mechanism of protein translocation across cell membranes is fundamentally important for cell biology. Several different type of protein translocation machineries called translocons have been so far identified and characterized. Among them, only bacterial-type SEC translocons have been extensively studied from the structural aspects simply because of some difficulty to obtain high amount of purified translocons of eukaryotic-type. In this study, we have successfully purified TIC translocon at the inner envelope membrane of chloroplast as well as its associated import motor complex.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (21 results)

All 2015 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (15 results) (of which Invited: 15 results) Remarks (3 results)

  • [Journal Article] The TIC complex uncovered: The alternative view on the molecular mechanism of protein translocation across the inner envelope membrane of chloroplasts2015

    • Author(s)
      Masato Nakai
    • Journal Title

      Biochim Biophys Acta.

      Volume: 1847 Issue: 9 Pages: 1-11

    • DOI

      10.1016/j.bbabio.2015.02.011

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 明らかになった葉緑体のトランスロコン2013

    • Author(s)
      地 真吾、平野 美奈子、井出 徹、中井 正人.
    • Journal Title

      細胞工学

      Volume: 32(8) Pages: 882-883

    • Related Report
      2013 Research-status Report
  • [Journal Article] Uncovering the Protein Translocon at the Chloroplast Inner Envelope Membrane2013

    • Author(s)
      Shingo Kikuchi
    • Journal Title

      SCIENCE

      Volume: 339 Issue: 6119 Pages: 571-574

    • DOI

      10.1126/science.1229262

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Unraveling the Mechanism of Protein Transport Across the Chloroplast Inner Envelope Membrane2015

    • Author(s)
      Masato Nakai
    • Organizer
      EMBO Conference   "Mechanisms and regulation of protein translocation"
    • Place of Presentation
      Croatia, Dubrovnik
    • Year and Date
      2015-03-21 – 2015-03-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Evolution of the Chloroplast Protein Import System2015

    • Author(s)
      Masato Nakai
    • Organizer
      Tokyo Tech-HHU Dusseldorf Joint Symposium on Photosynthesis as a New Chemical Resource
    • Place of Presentation
      東京 港区 東工大キャンパスイノベーションセンター
    • Year and Date
      2015-03-04 – 2015-03-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Unraveling the Mechanism of Protein Transport across the Chloroplast Inner Envelope Membrane2014

    • Author(s)
      Masato Nakai
    • Organizer
      The Finland-Japan Joint Research Seminar on Photosynthesis
    • Place of Presentation
      北海道 札幌市 定山渓万世閣ホテルミリオーネ
    • Year and Date
      2014-10-09 – 2014-10-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Reevaluation of the involvement of the "old" Tic proteins in chloroplast protein import2014

    • Author(s)
      Masato Nakai
    • Organizer
      The International Symposium on the Regulation of Photosynthetic Function
    • Place of Presentation
      中国 桂林
    • Year and Date
      2014-08-16 – 2014-08-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Reevaluation of the involvement of the "old" Tic proteins in chloroplast protein import2014

    • Author(s)
      Masato Nakai
    • Organizer
      The 16th Annual Meeting of The French Society of Photosynthesis
    • Place of Presentation
      France, Paris
    • Year and Date
      2014-04-14 – 2014-04-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The evolution of the chloroplast protein import system2014

    • Author(s)
      Masato Nakai
    • Organizer
      A DYNAMO LABEX SYMPOSIUM   "Evolution, biogenesis and dynamics of energy transducing membranes"
    • Place of Presentation
      France, Paris, Maison des Oceans, Institut Oceanographique
    • Year and Date
      2014-04-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Unraveling the mechanism of protein transport across the chloroplast inner envelope membrane2014

    • Author(s)
      Masato Nakai
    • Organizer
      East Asian Cell Biology Conference
    • Place of Presentation
      韓国 釜山 POSTECH
    • Year and Date
      2014-04-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] A novel TIC translocon-associated import motor?2013

    • Author(s)
      中井 正人
    • Organizer
      EMBO Conference "From Structure to Function of Translocation Machines"
    • Place of Presentation
      クロアチア Dubrovnik
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] New evolutionary insights into the chloroplast protein import system2013

    • Author(s)
      中井 正人
    • Organizer
      The 12th   International Forum on Infection and Immunity
    • Place of Presentation
      淡路島 夢舞台国際会議場
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 葉緑体タンパク質輸送と光合成超分子複合体の形成2013

    • Author(s)
      中井 正人
    • Organizer
      第77回 日本植物学会年会 シンポジウム   "光合成の超分子複合体の構造と動態"
    • Place of Presentation
      札幌 北海道大学高等教育推進機構
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 葉緑体タンパク質輸送装置の確立・進化2013

    • Author(s)
      中井 正人
    • Organizer
      第36回 日本分子生物学会年会 ワークショップ   "寄生・共生におけるゾンビ化機構の分子生物学的解析"
    • Place of Presentation
      神戸 国際会議場
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 葉緑体内包膜の新奇な蛋白質輸送装置:葉緑体進化の新たなる謎2013

    • Author(s)
      Masato Nakai
    • Organizer
      第15回 植物オルガネラワークショップ
    • Place of Presentation
      岡山 オルガホール
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Uncovering the protein translocon at the chloroplast inner envelope2012

    • Author(s)
      Masato Nakai
    • Organizer
      第12回 日本蛋白質科学会年会シンポジウム
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Uncovering the protein translocon at the chloroplast inner envelope2012

    • Author(s)
      Masato Nakai
    • Organizer
      Gordon Conference: Mitochondria & Chloroplasts
    • Place of Presentation
      アメリカ スミスフィールド
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 一次共生進化の初期過程でのタンパク質輸送装置の確立・進化2012

    • Author(s)
      Masato Nakai
    • Organizer
      第76回 日本植物学会 シンポジウム
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks] 中井准教授グループの研究紹介

    • URL

      http://www.protein.osaka-u.ac.jp/enzymology/nakaiJ.html

    • Related Report
      2013 Research-status Report
  • [Remarks] 大阪大学 最新研究成果リリース 植物の葉緑体に蛋白質を運び入れる分子装置を同定

    • URL

      http://www.osaka-u.ac.jp/ja/news/ResearchRelease/2013/02/20130201_1

    • Related Report
      2013 Research-status Report
  • [Remarks] 大阪大学蛋白質研究所最新研究成果 陸上植物の進化の謎 解明に一歩

    • URL

      http://www.protein.osaka-u.ac.jp/jpn/achievement/papers/post-6.php

    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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