Experimental development a new cryo-holder suitable to electron cryo-tomography

• Project/Area Number: 24657075
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: YASUNAGA Takuo 九州工業大学, 大学院・情報工学研究院, 教授 (60251394)
• Project Period (FY): 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: クライオ電子顕微鏡 / 構造生物学 / 電子顕微鏡法 / 試料ホルダー / 蛋白質構造解析

Research Abstract

The new cryo-holder suited to electron cryo-tomography was planed by using low-vibration cryo-pump and has been developed. Furthermore, conventional liquid-nitrogen-type cryo-holder was tested and successfully achieved a lower-temperature stage than conventional one by drawing vacuum with vacuum pumps. Hoses for vacuum drawing were important for less vibration of the stage and better hoses give the stable stage to obtain electro micrographs. Currently we are planning to develop a new holder by combining both techniques.

Research Products

• [Journal Article] Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans. (2013)
  • Author(s): Jing, Y., Dan, L. C., Shiori, T., Kotaro, K., Takuo, Y., Shinji, H. & Kang, S.
  • Journal Title: eLife Sciences 2.
  • Peer Reviewed
• [Journal Article] Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans (2013)
  • Author(s): Jing Yan
  • Journal Title: eLIFE Volume: 2
  • DOI: 10.7554/elife.00133
  • Peer Reviewed
• [Journal Article] Enhanced detection efficiency of genetically encoded tag allows the visualization of monomeric proteins by electron microscopy (2012)
  • Author(s): Fukunaga, Y., Higashihara, A., Nishino, Y., Yasunaga, T., Jin, M. & Miyazawa, A
  • Journal Title: Journal of Electron Microscopy Volume: 61 Pages: 229-236
  • Peer Reviewed
• [Journal Article] Tilt-angle measurement of a sample stage using a capacitive liquid-based inclinometer. (2012)
  • Author(s): Matoba, K., Takagi, J., Yasunaga, T., Jinnai, H. & Iwasaki, K.
  • Journal Title: Journal of Electron Microscopy Volume: 61 Pages: 193-198
  • Peer Reviewed
• [Journal Article] Tilt-angle measurement of a sample stage using a capacitive liquid-based inclinometer (2012)
  • Author(s): M.Matoba, et al
  • Journal Title: J.Electron.Microsc. Volume: 61(3) Pages: 193-198
  • DOI: 10.1093/jmicro/dfs034
  • Peer Reviewed
• [Journal Article] Enhanced detection efficiency of genetically encoded tag allows the visualization of monomeric proteins by electron microscopy (2012)
  • Author(s): Yuko Fukunaga
  • Journal Title: Microscopy Volume: 61 Issue: 4 Pages: 229-236
  • DOI: 10.1093/jmicro/dfs043
  • Peer Reviewed
• [Remarks]
  • URL: http://www.yasunaga-lab.bio.kyutech.ac.jp
• [Remarks] 安永研究室HP
  • URL: http://www.yasunaga-lab.bio.kyutech.ac.jp/