Crsytallization chaperon by DNA clamp for protein crystallography
Project/Area Number |
24657076
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
|
Research Institution | University of Shizuoka (2013) Yokohama City University (2012) |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | X線結晶構造解析 / タンパク質 / 結晶化 / タンパク質の結晶化 / 構造生物学 / 結晶化シャペロン / DNAクランプ / タンパク質結晶 |
Research Abstract |
Crystallization of proteins is the most crucial step in the protein crystallography. To date, various crystallization chaperon for proteins are developed. However, there is no versatile chaperon applicable for various proteins. We consider that DNA clamp molecule is usable for crystallization chaperon because DNA clamp adopt symmetric quaternary structure and easily crystallized. Our chaperon will be an option for protein crystallization. In this works, we constructed three types of expression vectors to produce chaperon-fused protein. We tested tree proteins and showed availability of our chaperon.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] A Small Molecule Inhibitor of Monoubiquitinated Proliferating Cell Nuclear Antigen (PCNA) inhibits Repair of Interstrand DNA Crosslink, enhances DNA Double-strand Break, and sensitizes Cancer Cells to Cisplatin2014
Author(s)
Akira Inoue, Sotaro Kikuchi, Asami Hishiki, Youming Shao, Richard Heath, Benjamin J. Evison, Marcelo Actis, Christine E. Canman, Hiroshi Hashimoto, & Naoaki Fujii
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Journal Title
J. Biol. Chem
Volume: 289
Issue: 10
Pages: 7109-7120
DOI
Related Report
Peer Reviewed
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[Journal Article] A Missense Mutation in Rev7 Disrupts Formation of Polζ, Impairing Mouse Development and Repair of Genotoxic Agent-induced DNA Lesions2014
Author(s)
Maryam Khalaj, Abdolrahim Abbasi, Hiroshi Yamanishi, Kouyou Akiyama, Shuso Wakitani, Sotaro Kikuchi, Michiko Hirose, Misako Yuzuriha, Masaki Magari, Heba A Degheidy, Kuniya Abe, Atsuo Ogura, Hiroshi Hashimoto, & Tetsuo Kunieda
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Journal Title
J Biol Chem
Volume: 289
Issue: 6
Pages: 3811-3824
DOI
Related Report
Peer Reviewed
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[Journal Article] Crystal structure of aldoxime dehydratase and its catalytic mechanism involved in carbon-nitrogen triple-bond synthesis2013
Author(s)
Nomura, J., Hashimoto, H., Ohta, T., Hashimoto, Y., Wada, K., Naruta, Y., Oinuma, K-I., & Kobayashi, M.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 110
Issue: 8
Pages: 2810-2815
DOI
Related Report
Peer Reviewed
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[Journal Article] Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex2013
Author(s)
N.Kuwabara, Y.Murayama, H.Hashimoto, Y.Kokabu, M.Ikeguchi, M.Sato, K.Mayanagi, Y.Tsutsui, H.Iwasaki,and T.Shimizu
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Journal Title
Structure
Volume: 20
Issue: 3
Pages: 440-449
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of a small molecule PCNA inhibitor that disrupts interactions with PIP-Box proteins and inhibits DNA replication2012
Author(s)
Chandanamali Punchihewa, Akira Inoue, Asami Hishiki, Yoshihiro Fujikawa, Michele Connelly, Benjamin Evison, Youming Shao, Richard Heath, Isao Kuraoka, Patrick Rodrigues, Hiroshi Hashimoto, Masanobu Kawanishi, Mamoru Sato, Takashi Yagi, Naoaki Fujii
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Journal Title
J Biol Chem
Volume: 287
Issue: 17
Pages: 14289-14300
DOI
Related Report
Peer Reviewed
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