| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Crystallization of proteins is the most crucial step in the protein crystallography. To date, various crystallization chaperon for proteins are developed. However, there is no versatile chaperon applicable for various proteins. We consider that DNA clamp molecule is usable for crystallization chaperon because DNA clamp adopt symmetric quaternary structure and easily crystallized. Our chaperon will be an option for protein crystallization. In this works, we constructed three types of expression vectors to produce chaperon-fused protein. We tested tree proteins and showed availability of our chaperon.
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