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Crsytallization chaperon by DNA clamp for protein crystallography

Research Project

Project/Area Number 24657076
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionUniversity of Shizuoka (2013)
Yokohama City University (2012)

Principal Investigator

HASHIMOTO Hiroshi  静岡県立大学, 薬学部, 教授 (40336590)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsX線結晶構造解析 / タンパク質 / 結晶化 / タンパク質の結晶化 / 構造生物学 / 結晶化シャペロン / DNAクランプ / タンパク質結晶
Research Abstract

Crystallization of proteins is the most crucial step in the protein crystallography. To date, various crystallization chaperon for proteins are developed. However, there is no versatile chaperon applicable for various proteins. We consider that DNA clamp molecule is usable for crystallization chaperon because DNA clamp adopt symmetric quaternary structure and easily crystallized. Our chaperon will be an option for protein crystallization. In this works, we constructed three types of expression vectors to produce chaperon-fused protein. We tested tree proteins and showed availability of our chaperon.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (25 results)

All 2014 2013 2012 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (17 results) (of which Invited: 3 results)

  • [Journal Article] A Small Molecule Inhibitor of Monoubiquitinated Proliferating Cell Nuclear Antigen (PCNA) inhibits Repair of Interstrand DNA Crosslink, enhances DNA Double-strand Break, and sensitizes Cancer Cells to Cisplatin2014

    • Author(s)
      Akira Inoue, Sotaro Kikuchi, Asami Hishiki, Youming Shao, Richard Heath, Benjamin J. Evison, Marcelo Actis, Christine E. Canman, Hiroshi Hashimoto, & Naoaki Fujii
    • Journal Title

      J. Biol. Chem

      Volume: 289 Issue: 10 Pages: 7109-7120

    • DOI

      10.1074/jbc.m113.520429

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Missense Mutation in Rev7 Disrupts Formation of Polζ, Impairing Mouse Development and Repair of Genotoxic Agent-induced DNA Lesions2014

    • Author(s)
      Maryam Khalaj, Abdolrahim Abbasi, Hiroshi Yamanishi, Kouyou Akiyama, Shuso Wakitani, Sotaro Kikuchi, Michiko Hirose, Misako Yuzuriha, Masaki Magari, Heba A Degheidy, Kuniya Abe, Atsuo Ogura, Hiroshi Hashimoto, & Tetsuo Kunieda
    • Journal Title

      J Biol Chem

      Volume: 289 Issue: 6 Pages: 3811-3824

    • DOI

      10.1074/jbc.m113.514752

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of aldoxime dehydratase and its catalytic mechanism involved in carbon-nitrogen triple-bond synthesis2013

    • Author(s)
      Nomura, J., Hashimoto, H., Ohta, T., Hashimoto, Y., Wada, K., Naruta, Y., Oinuma, K-I., & Kobayashi, M.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 110 Issue: 8 Pages: 2810-2815

    • DOI

      10.1073/pnas.1200338110

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex2013

    • Author(s)
      N.Kuwabara, Y.Murayama, H.Hashimoto, Y.Kokabu, M.Ikeguchi, M.Sato, K.Mayanagi, Y.Tsutsui, H.Iwasaki,and T.Shimizu
    • Journal Title

      Structure

      Volume: 20 Issue: 3 Pages: 440-449

    • DOI

      10.1016/j.str.2012.01.005

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of a small molecule PCNA inhibitor that disrupts interactions with PIP-Box proteins and inhibits DNA replication2012

    • Author(s)
      Chandanamali Punchihewa, Akira Inoue, Asami Hishiki, Yoshihiro Fujikawa, Michele Connelly, Benjamin Evison, Youming Shao, Richard Heath, Isao Kuraoka, Patrick Rodrigues, Hiroshi Hashimoto, Masanobu Kawanishi, Mamoru Sato, Takashi Yagi, Naoaki Fujii
    • Journal Title

      J Biol Chem

      Volume: 287 Issue: 17 Pages: 14289-14300

    • DOI

      10.1074/jbc.m112.353201

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural basis for the inhibition of xyloglucan-specific endo-β-1,4-glucanase (XEG) by XEG- protein inhibitor2012

    • Author(s)
      Takuya Yoshizawa
    • Journal Title

      J Biol Chem

      Volume: 287 Issue: 22 Pages: 18710-18716

    • DOI

      10.1074/jbc.m112.350520

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Crystallization and X-ray diffraction analysis of the ternary complex of the C-terminal domain of human REV1 in complex with REV7 bound to a REV3 fragment involved in translesion DNA synthesis2012

    • Author(s)
      Sotaro Kikuchi
    • Journal Title

      Acta Crystallogr Sect F Struct Biol Cryst Commun

      Volume: 68 Issue: 8 Pages: 962-964

    • DOI

      10.1107/s1744309112032435

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural basis of recruitment of DNA polymerase z by interaction between REV1 and REV72012

    • Author(s)
      Sotaro Kikuchi
    • Journal Title

      J Biol Chem

      Volume: 287 Issue: 40 Pages: 33847-33852

    • DOI

      10.1074/jbc.m112.396838

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 損傷乗り越えDNA合成におけるポリメラーゼ相互作用の構造基盤2013

    • Author(s)
      橋本博
    • Organizer
      第12回NRGICがん・ゲノム重点セミナー
    • Place of Presentation
      長崎大学
    • Year and Date
      2013-07-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞周期を制御するREV7/Mad2l2と赤痢菌IpaBとの相互作用の構造基盤2013

    • Author(s)
      菊池壮太郎、原幸大、清水敏之、佐藤衛、橋本博
    • Organizer
      日本結晶学会年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
  • [Presentation] Structural study of REV7, the adaptor protein coordinating REV1 and REV3 polymerases in translesion DNA synthesis2013

    • Author(s)
      Sotato Kikuchi, Kodai Hara, Toshiyuki Shimizu, Mamoru Sato, Hiroshi Hashimoto
    • Organizer
      Structural Life Science 7th International Conference on Structural Genomics
    • Place of Presentation
      sapporo
    • Related Report
      2013 Annual Research Report
  • [Presentation] 修復・転写・細胞周期に関わるMad2L2シグナリング複合体の構造生物学2013

    • Author(s)
      橋本博
    • Organizer
      新学術領域「構造細胞生物学」班会議
    • Place of Presentation
      山梨
    • Related Report
      2013 Annual Research Report
  • [Presentation] REV7-REV3複合体構造から示唆される REV7/Mad2L2と赤痢菌タンパク質 IpaB との相互作用2013

    • Author(s)
      菊池壮太郎、原幸大、清水敏之、佐藤衛、橋本博
    • Organizer
      日本蛋白質科学会年会
    • Place of Presentation
      鳥取
    • Related Report
      2013 Annual Research Report
  • [Presentation] REV1-REV7-REV3三者複合体の結晶構造から明らかになったDNAポリメラーゼζのリクルート機構2013

    • Author(s)
      菊池 壮太郎
    • Organizer
      第30回PFシンポジウム
    • Place of Presentation
      つくは国際会議場, 茨城
    • Related Report
      2012 Research-status Report
  • [Presentation] Crystal structures of human PCNA in complex with small molecule inhibitors for cancer therapeutics2012

    • Author(s)
      H iroshi Hashimoto, Sotaro Kikuchi, Asami Hishiki, Mamoru Sato, Chandanamali Punchihewa, Naoaki Fujii
    • Organizer
      AsCA 12/CRYSTAL 28
    • Place of Presentation
      Adelaide Convention Centre, Adelaide, Australia
    • Related Report
      2013 Final Research Report
  • [Presentation] 抗がん剤併用薬開発に向けたPCNAと阻害剤との複合体構造2012

    • Author(s)
      菊池壮太郎、菱木麻美、Chandanamali Punchihewa、藤井直明、佐藤衛、橋本博
    • Organizer
      第12回日本蛋白質科学会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] 抗がん剤併用薬開発に向けた PCNAと阻害剤との複合体構造2012

    • Author(s)
      菊池 壮太郎
    • Organizer
      第12回日本蛋白質科学会
    • Place of Presentation
      名古屋国際会議場, 愛知
    • Related Report
      2012 Research-status Report
  • [Presentation] REV1-REV7-REV3相互作用の構造基盤2012

    • Author(s)
      橋本 博
    • Organizer
      遺伝研研究集会
    • Place of Presentation
      国立遺伝学研究所, 静岡
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 損傷乗り越えDNA合成に関わるREV1-REV7-REV3三者複合体の結晶構造解析2012

    • Author(s)
      菊池 壮太郎
    • Organizer
      日本結晶学会年会
    • Place of Presentation
      東北大学 片平キャンパス, 宮城
    • Related Report
      2012 Research-status Report
  • [Presentation] Structural basis of recruitment of DNA polymerase ζ by interaction between REV1 and REV72012

    • Author(s)
      Sotaro Kikuchi
    • Organizer
      The 8th 3R Symposium
    • Place of Presentation
      Awaji Yumebutai International Conference Center, Hyogo
    • Related Report
      2012 Research-status Report
  • [Presentation] Structural basis of recruitment of DNA polymerase zeta by interaction between REV1 and REV72012

    • Author(s)
      Sotaro Kikuchi
    • Organizer
      AsCA 12/CRYSTAL 28
    • Place of Presentation
      Adelaide Convention Centre, Australia
    • Related Report
      2012 Research-status Report
  • [Presentation] Crystal structures of human PCNA in complex with small molecule inhibitors for cancer therapeutics2012

    • Author(s)
      Hiroshi Hashimoto
    • Organizer
      AsCA 12/CRYSTAL 28
    • Place of Presentation
      Adelaide Convention Centre, Australia
    • Related Report
      2012 Research-status Report
  • [Presentation] REV1-REV7-REV3三者複合体の結晶構造から明らかになったDNAポリメラーゼζのリクルート機構の構造基盤2012

    • Author(s)
      菊池 壮太郎
    • Organizer
      第35回 日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場, 福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] DNA損傷があっても複製を続けるためのDNAポリメラーゼ相互作用

    • Author(s)
      橋本博
    • Organizer
      X線の会
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 損傷乗り越えDNA合成におけるポリメラーゼ相互作用の構造基盤

    • Author(s)
      橋本博
    • Organizer
      NRGICがん・ゲノム重点セミナー
    • Place of Presentation
      長崎
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2013-05-31   Modified: 2019-07-29  

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