| Project/Area Number |
24657078
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | High Energy Accelerator Research Organization |
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Principal Investigator |
NOBUTAKA Shimizu 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (20450934)
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| Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Masato 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (00342600)
IGARASHI Noriyuki 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (80300672)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 生体膜変形 / 時分割X線小角散乱 / ストップトフロー / X線溶液散乱 / 時分割測定 / GUV / 脂質膜変形 / X線小角散乱 / 生体膜 |
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| Research Abstract |
Some proteins transform an intracellular organelle and maintain structure. It is important to study those interactions in order to understand the structure and the function of the organelle. Then, a stopped-flow was installed in the small-angle X-ray scattering beamline at the Japanese synchrotron facility, Photon Factory, and the structural change of Giant unilamellar vesicle (GUV) interacted by the BAR domain protein was measured by time-resolve method. As a result, although it was very preliminary analysis, it was suggested the structure of GUV was changed with BAR domain in the space scale of around 125 angstrom within 210 seconds.
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