Time-resolved small-angle X-ray scattering of GUV deformed by F-BAR domain
Project/Area Number |
24657078
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
|
Research Institution | High Energy Accelerator Research Organization |
Principal Investigator |
NOBUTAKA Shimizu 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (20450934)
|
Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Masato 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (00342600)
IGARASHI Noriyuki 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (80300672)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 生体膜変形 / 時分割X線小角散乱 / ストップトフロー / X線溶液散乱 / 時分割測定 / GUV / 脂質膜変形 / X線小角散乱 / 生体膜 |
Research Abstract |
Some proteins transform an intracellular organelle and maintain structure. It is important to study those interactions in order to understand the structure and the function of the organelle. Then, a stopped-flow was installed in the small-angle X-ray scattering beamline at the Japanese synchrotron facility, Photon Factory, and the structural change of Giant unilamellar vesicle (GUV) interacted by the BAR domain protein was measured by time-resolve method. As a result, although it was very preliminary analysis, it was suggested the structure of GUV was changed with BAR domain in the space scale of around 125 angstrom within 210 seconds.
|
Report
(3 results)
Research Products
(25 results)
-
[Journal Article] Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling2013
Author(s)
Hirai, M., Kimura, R., Takeuchi, K., Hagiwara, Y., Kawai-Hirai, R., Ohta, N., Igarashi, N. and Shimizu, N.
-
Journal Title
J. Synchrotron Rad.
Volume: 20
Pages: 869-874
Related Report
Peer Reviewed
-
[Journal Article] Refurbishing of Small-Angle X-ray Scattering Beamline, BL-6A at the Photon Factory2013
Author(s)
Shimizu, N., Mori, T., Igarashi, N., Ohta, H., Nagatani, Y., Kosuge, T. and Ito, K.
-
Journal Title
J. Phys. : Conf. Ser.
Volume: 425
Pages: 202008-202008
Related Report
Peer Reviewed
-
[Journal Article] New high-brilliance beamline BL-15A of the Photon Factory2013
Author(s)
Igarashi, N., Shimizu, N., Koyama, A., Mori, T., Ohta, H., Niwa, Y., Nitani, H., Abe, H., Nomura, M., Shioya, T., Tsuchiya, K. and Ito, K.
-
Journal Title
J. Phys. : Conf. Ser.
Volume: 425
Pages: 72016-72016
Related Report
Peer Reviewed
-
[Journal Article] Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling2013
Author(s)
Hirai, M., Kimura, R., Takeuchi, K., Hagiwara, Y., Kawai-Hirai, R., Ohta, N., Igarashi, N. and Shimizu, N
-
Journal Title
Journal of Synchrotron Radiation
Volume: 20
Issue: 6
Pages: 869-874
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] New high-brilliance beamline BL-15A of the Photon Factory2013
Author(s)
N Igarashi, N Shimizu, A Koyama, T Mori, H Ohta, Y Niwa, H Nitani, H Abe, M Nomura, T Shioya, K Tsuchiya and K Ito
-
Journal Title
Journal of Physics: Conference Series
Volume: 425
Issue: 7
Pages: 72016-72016
DOI
Related Report
Peer Reviewed
-
[Journal Article] The binding mechanism of eIF2βwith its partner proteins, eIF5 and eIF2βε2012
Author(s)
Gai, Z., Kitagawa, Y., Tanaka, Y., Shimizu, N., Komoda, K., Tanaka, I. and Yao M.
-
Journal Title
Biochem. Biophys. Res. Commun.
Volume: 423
Pages: 515-519
Related Report
Peer Reviewed
-
[Journal Article] The binding mechanism of eIF2β with its partner proteins, eIF5 and eIF2Bε2012
Author(s)
Gai, Z., Kitagawa, Y., Tanaka, Y., Shimizu, N., Komoda, K., Tanaka, I. and Yao M.
-
Journal Title
Biochem. Biophys. Res. Commun.
Volume: 423
Pages: 515-519
Related Report
Peer Reviewed
-
-
-
-
[Presentation] Improvement and Upgrade Plan of Small-Angle X-ray Scattering Beamlines at Photon Factory2013
Author(s)
Shimizu, N., Mori, T., Ohta, H., Nagatani, Y., Saijyo, S., Kamijyo, A., Kosuge, T. and Igarashi, N.
Organizer
11th International Conference on Biology and Synchrotron Radiation
Place of Presentation
Hamburg、Germany(ポスター)
Year and Date
2013-09-09
Related Report
-
[Presentation] BL-15A : Semi-microbeam XAFS/XRF and High- brilliance SAXS beamline at Photon Factory2013
Author(s)
Igarashi, N., Koyama, A., Mori, T., Ohta, H., Kamijo, A., Saijyo, S., Niwa, Y., Nitani, H., Abe, H. and Shimizu, N.
Organizer
11th International Conference on Biology and Synchrotron Radiation
Place of Presentation
Hamburg、Germany(ポスター)
Year and Date
2013-09-09
Related Report
-
-
[Presentation] Improvement and Upgrade Plan of Small-Angle X-ray Scattering Beamlines at Photon Factory2013
Author(s)
Shimizu, N., Mori, T., Ohta, H., Nagatani, Y., Saijyo, S., Kamijyo, A., Kosuge, T. and Igarashi, N.
Organizer
11th International Conference on Biology and Synchrotron Radiation
Place of Presentation
Grand Elysee Hotel Hamburg, Germany
Related Report
-
[Presentation] BL-15A: Semi-microbeam XAFS/XRF and High-brilliance SAXS beamline at Photon Factory2013
Author(s)
Igarashi, N., Koyama, A., Mori, T., Ohta, H., Kamijo, A., Saijyo, S., Niwa, Y., Nitani, H., Abe, H. and Shimizu, N.
Organizer
11th International Conference on Biology and Synchrotron Radiation
Place of Presentation
Grand Elysee Hotel Hamburg, Germany
Related Report
-
-
-
[Presentation] Current Status of Small Angle X-ray Scattering Beamlines at Photon Factory2012
Author(s)
Shimizu, N., Mori, T., Igarashi, N., Ohta, H., Nagatani, Y., Kosuge, T. and Ito, K.
Organizer
15th International Small-Angle Scattering conference (SAS2012)
Place of Presentation
Sydney、Australia(ポスター)
Year and Date
2012-11-20
Related Report
-
[Presentation] Current Status of Small Angle X-ray Scattering Beamlines at Photon Factory2012
Author(s)
Shimizu, N., Mori, T., Igarashi, N., Ohta, H., Nagatani, Y., Kosuge, T. and Ito, K.
Organizer
第50回日本生物物理学会年会
Place of Presentation
名古屋大学(ポスター)
Year and Date
2012-09-24
Related Report
-
[Presentation] Development of Small-Angle X-ray Scattering Beamline, BL-6A at the Photon Factory2012
Author(s)
Shimizu, N., Mori, T., Igarashi, N., Ohta, H., Nagatani, Y., Kosuge, T. and Ito, K.
Organizer
11th International Conference on Synchrotron Radiation Instrumentation (SRI2012)
Place of Presentation
Lyon, France(ポスター)
Year and Date
2012-07-11
Related Report
-
[Presentation] Development of Small-Angle X-ray Scattering Beamline, BL-6A at the Photon Factory2012
Author(s)
Shimizu, N., Mori, T., Igarashi, N., Ohta, H., Nagatani, Y., Kosuge, T. and Ito, K.
Organizer
11th International Conference on Synchrotron Radiation Instrumentation (SRI2012)
Place of Presentation
Center of Congress (Lyon, France)
Related Report
-
[Presentation] Current Status of Small Angle X-ray Scattering Beamlines at Photon Factory2012
Author(s)
Shimizu, N., Mori, T., Igarashi, N., Ohta, H., Nagatani, Y., Kosuge, T. and Ito, K.
Organizer
第50回日本生物物理学会年会
Place of Presentation
名古屋大学(名古屋)
Related Report
-
-
[Presentation] Current Status of Small Angle X-ray Scattering Beamlines at Photon Factory2012
Author(s)
Shimizu, N., Mori, T., Igarashi, N., Ohta, H., Nagatani, Y., Kosuge, T. and Ito, K.
Organizer
15th International Small-Angle Scattering conference (SAS2012)
Place of Presentation
Sydney Convention & Exhibition Centre (Sydney, Australia)
Related Report