Molecular mechanism that links a nutrient-responsive miRNA with clock genes

• Project/Area Number: 24657081
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: KATADA TOSHIAKI 東京大学, 薬学研究科(研究院), 教授 (10088859)
• Co-Investigator(Kenkyū-buntansha): FUKUYAMA Masamitsu 東京大学, 大学院薬学系研究科, 助教 (20422389)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: microRNA / 時計遺伝子 / 栄養感知 / 線虫 / シグナル伝達 / 栄養応答

Research Abstract

Although the microRNA (miRNA) miR-92 and its paralogues have been known to be associated with tumorigenesis, their physiological role and the signaling pathway in which they function remain to be delineated. In this study, we explored the relationship between the miR-92 family of miRNAs and the clock genes, using the nematode C. elegans and mammalian cultured cells.
We found several lines of evidence suggesting that C. elegans miR-92 orthologue, miR-235 and the clock gene Period2 ortholog lin-42 negatively regulate each other to determine the timing of differentiation in progenitor cells. On the other hand, miR-92 and one of its paralogues did not show oscillatory expression pattern in cultured rodent fibroblasts.

Research Products

• [Journal Article] 幹・前駆細胞の栄養応答とその分子機構の最前線インスリン経路とマイクロRNAの関与 (2014)
  • Author(s): 福山征光
  • Journal Title: ファルマシア Volume: 50 Pages: 512-516
  • NAID: 130005160520
  • URL: http://farumashia.pharm.or.jp/mokuji/2014/50-06.html
• [Journal Article] The microRNA miR-235 couples blast cell quiescence to the nutritional state. (2013)
  • Author(s): Kasuga H, Fukuyama M, Kitazawa A, Kontani K. Katada T.
  • Journal Title: Nature Volume: 497 Issue: 7450 Pages: 503-506
  • DOI: 10.1038/nature12117
  • Peer Reviewed
• [Journal Article] Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia (2013)
  • Author(s): Su S, Phua SC, DeRose R, Chiba S, Narita K, Kalugin PN, Katada T, Kontani K, Takeda S, Inoue T.
  • Journal Title: Nat. Methods Volume: 10 Issue: 11 Pages: 1105
  • DOI: 10.1038/nmeth.2647
  • Peer Reviewed
• [Journal Article] Active transport and diffusion barriers restrict Joubert Syndrome-associated ARL13B/ARL-13 to an Inv-like ciliary membrane subdomain. (2013)
  • Author(s): Cevik S, Sanders AA, Van Wijk E, Boldt K, Clarke L, van Reeuwijk J, Hori Y, Horn N, Hetterschijt L, Wdowicz A, Mullins A, Kida K, Kaplan OI, van Beersum SE, Man Wu K, Letteboer SJ, Mans DA, Katada T, Kontani K, Ueffing M, Roepman R, Kremer H, Blacque OE.
  • Journal Title: PLoS Genet. Volume: 9 Issue: 12 Pages: e1003977-e1003977
  • DOI: 10.1371/journal.pgen.1003977
  • Peer Reviewed / Open Access
• [Journal Article] (2013)
  • Author(s): Kawazu M, Ueno T, Kontani K, Ogita Y, Ando M, Fukumura K, Yamato A, Soda M, Takeuchi K, Miki Y, Yamaguchi H, Yasuda T, Naoe T,Yamashita Y, Katada T, Choi YL, Mano H,
  • Journal Title: Proc Natl Acad Sci U S A Volume: 110 Issue: 8 Pages: 3029-3034
  • DOI: 10.1073/pnas.1216141110
  • Peer Reviewed
• [Journal Article] Arl8/ARL-8 functions in apoptotic cell removal by mediating phagolysosome formation in C. elegans. (2013)
  • Author(s): Sasaki A
  • Journal Title: Mol Biol Cell. Volume: in press
  • Peer Reviewed
• [Journal Article] RINL, guanine nucleotide exchange factor Rab5-subfamily, is involved in the EphA8-degradation pathway with odin (2012)
  • Author(s): Kajiho, H., et al.
  • Journal Title: PLoS One Volume: 7 Issue: 1 Pages: e30575-e30575
  • DOI: 10.1371/journal.pone.0030575
  • Peer Reviewed
• [Journal Article] A novel acetylation cycle of transcription co-activator Yes-associated protein that is downstream of Hippo pathway is triggered in response to SN2 alkylating agents. (2012)
  • Author(s): Hata S
  • Journal Title: J. Biol. Chem. Volume: 287 Pages: 22089-22098
  • Peer Reviewed
• [Journal Article] Neuronally expressed Ras-family GTPase Di-Ras modulates synaptic activity in Caenorhabditis elegans (2012)
  • Author(s): Tada M, Gengyo-Ando K, Kobayashi T, Fukuyama M, Mitani S, Kontani K, Katada T
  • Journal Title: Genes Cells Volume: 17(9) Issue: 9 Pages: 778-89
  • DOI: 10.1111/j.1365-2443.2012.01627.x
  • Peer Reviewed
• [Journal Article] C. elegans AMPKs promote survival and arrest germline development during nutrient stress. (2012)
  • Author(s): Fukuyama M., et al.
  • Journal Title: Bio. Open Volume: 1 Issue: 10 Pages: 929-936
  • DOI: 10.1242/bio.2012836
  • Peer Reviewed
• [Journal Article] Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities (2012)
  • Author(s): Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue K. A., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Ta
  • Journal Title: Structure Volume: 20 Issue: 9 Pages: 1585
  • DOI: 10.1016/j.str.2012.06.017
  • Peer Reviewed
• [Journal Article] Biological role of the two overlapping poly(A)-binding protein interacting motifs 2 (PAM2) of eukaryotic releasing factor eRF3 in mRNA decay. (2012)
  • Author(s): Osawa M, Hosoda N, Nakanishi T, Uchida N, Kimura T, Imai S, Machiyama A, Katada T, Hoshino S, Shimada I.
  • Journal Title: RNA Volume: 18 Issue: 11 Pages: 1957-1967
  • DOI: 10.1261/rna.035311.112
  • Peer Reviewed
• [Journal Article] The inhibitory G protein G(i) identified as pertussis toxin-catalyzed ADP-ribosylation. (2012)
  • Author(s): Katada T
  • Journal Title: Biol. Pharm. Bull. Volume: 35 Pages: 2103-2111
  • NAID: 130002480519
  • Peer Reviewed
• [Journal Article] Critical roles of type III phosphatidylinositol phosphate kinase in murine embryonic visceral endoderm and adult intestine (2012)
  • Author(s): Takasuga , Wada et al.
  • Journal Title: PNAS 110 Volume: 1726-1731 Issue: 5 Pages: 1726-31
  • DOI: 10.1073/pnas.1213212110
  • Peer Reviewed
• [Presentation] 線虫のマイクロRNA miR-235は幼生発生開始の栄養チェックポイントとして機能する (2013)
  • Author(s): 福山征光、春日秀文、北澤文、小川拓也、粂優彦、紺谷圏二、堅田利明
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-04
• [Presentation] microRNAであるmiR-235は前駆細胞の休眠と栄養状態を結びつける (2013)
  • Author(s): 福山征光、春日秀文、北澤文、紺谷圏ニ、堅田利明
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-28
• [Presentation] The microRNA miR-235 suspends growth and development during starvation (2013)
  • Author(s): Fukuyama, M., Kasuga, H., Kitazawa, A., Kume, M., Ogawa, T., Kontani, K., Katada, T.
  • Organizer: 第46回日本発生生物学会
  • Place of Presentation: 松江
  • Year and Date: 2013-05-31
• [Presentation] インスリン経路を介した芽細胞休眠制御に関与するmicroRNA mir-235の同定と解析 (2012)
  • Author(s): 春日秀文、福山征光、北澤文、紺谷圏二、堅田利明
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] 休眠時に高発現して発生停止を維持するmicroRNA (2012)
  • Author(s): 福山征光、春日秀文、北澤文、紺谷圏二、堅田利明
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] 線虫のマイクロRNA miR-235は幼生発生開始の栄養チェックポイントとして機能する
  • Author(s): 福山征光、春日秀文、北澤文、小川拓也、粂優彦、紺谷圏二、堅田利明
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県・神戸市）
  • Invited
• [Presentation] microRNAであるmiR-235は前駆細胞の休眠と栄養状態を結びつける
  • Author(s): 福山 征光、春日 秀文、北澤 文、紺谷 圏ニ、堅田 利明
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山大学（岡山県・岡山市）
• [Presentation] The microRNA mir-235 suspends growth and development during starvation
  • Author(s): 福山 征光、春日 秀文、北澤 文、粂優彦、小川拓也、紺谷 圏ニ、堅田 利明
  • Organizer: 第46回日本発生生物学会
  • Place of Presentation: 島根県立産業交流会館（島根県・松江市）
• [Presentation] Ethanol and essential amino acids can trigger postembryonic development through the insulin/IGF pathway.
  • Author(s): Fukuyama M
  • Organizer: 5th East Asia C. elegans meeting.
  • Place of Presentation: 台北、台湾
• [Presentation] 繊毛形成・機能に介在するARLファミリー低分子量Gタンパク質の解析
  • Author(s): 紺谷圏二
  • Organizer: 第3回繊毛研究会
  • Place of Presentation: 東京
• [Presentation] 低分子量G蛋白質Sar1の活性制御によるコラーゲン分泌機構の解明
  • Author(s): 齋藤 康太
  • Organizer: 第11回生命科学研究会
  • Place of Presentation: 秋田
• [Presentation] 休眠時に高発現して発生停止を維持するmicroRNA
  • Author(s): 福山 征光
  • Organizer: 日本動物学会 第83回大会
  • Place of Presentation: 大阪
• [Presentation] インスリン経路を介した芽細胞休眠制御に関与するmicroRNAmir-235の同定と解析
  • Author(s): 春日 秀文
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] アティピカルRasファミリーGタンパク質Di-Rasの機能解析
  • Author(s): 紺谷 圏二
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Remarks] 春日秀文・福山征光.ライフサイエンス新着論文レビュー"First Author's"
  • URL: http://first.lifesciencedb.jp/archives/7163
• [Remarks] 福山 征光.日本学術振興会ウェブページ「事業の成果」
  • URL: https://www.jsps.go.jp/seika/2013/vol3_003.html
• [Remarks] 福山 征光.科研費NEWS 2013年度VOL.3 P.17.「マイクロRNAによる前駆細胞の栄養応答性調節機構の研究」
  • URL: http://www.jsps.go.jp/j-grantsinaid/22_letter/data/news_2013_vol3/news_2013_vol3.pdf
• [Remarks] 福山 征光、春日 秀文、北澤 文、小川 拓也、粂 優彦、紺谷 圏二、堅田 利明.東京大学プレスリリース(平成25年5月8日)「栄養不足が発育をさまたげるしくみ―低栄養状態に応答し前駆細胞の活性化を阻害する遺伝子を発見―」
  • URL: http://www.u-tokyo.ac.jp/public/public01_250508_02_j.html
• [Remarks] 東京大学・大学院薬学系研究科 生理化学教室ホームページ
  • URL: http://www.f.u-tokyo.ac.jp/~seiri/
• [Remarks] 東京大学プレスリリース
  • URL: http://www.u-tokyo.ac.jp/public/public01_250508_02_j.html
• [Remarks] 生理化学教室のホームページ
  • URL: http://www.f.u-tokyo.ac.jp/~seiri/