| Project/Area Number |
24657083
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SUZUKI Kuninori 東京大学, 新領域創成科学研究科, 准教授 (20373194)
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| Co-Investigator(Renkei-kenkyūsha) |
NODA Nobuo 微生物化学研究所, 主席研究員 (40396297)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 細胞内タンパク質分解 / オートファジー / 選択的オートファジー / オートファゴソーム / 出芽酵母 / タンパク質分解 / 膜輸送 / AIM |
|---|
| Research Abstract |
Macroautophagy (autophagy) is a bulk protein-degradation system ubiquitously conserved in eukaryotic cells. During autophagy, cytoplasmic components are enclosed in a membrane compartment, called an autophagosome. In this study, we developed a method for monitoring intact autophagosomes ex vivo by detecting the fluorescence of GFP-fused aminopeptidase I, the best-characterized selective cargo of autophagosomes in Saccharomyces cerevisiae. A combination of LC-MS/MS with subsequent statistical analyses revealed a list of autophagosome cargo proteins. The methods we describe will be useful for analyzing the mechanisms and physiological significance of Atg11-independent selective autophagy.
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