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Analyses of the molecular interaction between epithelial organogenesis and immune system via specific antigens

Research Project

Project/Area Number 24657086
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionNiigata University

Principal Investigator

IZUTSU YUMI  新潟大学, 自然科学系, 准教授 (20301921)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords発生・分化 / 免疫学 / 蛋白質 / 発現制御 / 動物 / アフリカツメガエル / 両生類の変態 / 幼生抗原 / 免疫 / 細胞死 / 皮膚 / T細胞 / タンパク質
Research Abstract

We previously found a novel protein, named Ouro, as a candidate for immune antigens from amphibian. In this study, to clarify molecular mechanisms underlying the body plan of vertebrate animals, we focused on the functions of acquired immune system vs specific self-antigens, i.e., Ouro proteins. First, we examined immune cell response to the self-organ that expresses Ouro proteins. Using our transgenic Xenopus line, we found that immune T cells immediately migrated into Ouro expressing cells in the tadpole tail by using a live-cell-tracking. Second, we obtained polyclonal rat antisera against Ouro proteins. Third, using these antisera, we have tried the immunohistochemical analyses, and shown that a unique intracellular localization was seen when the Ouro proteins are recognized by immune cells.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (11 results)

All 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (8 results) (of which Invited: 4 results) Remarks (1 results)

  • [Journal Article] おたまじゃくしの尾の消失―免疫学的な観点から見る動物の体作り2012

    • Author(s)
      井筒ゆみ
    • Journal Title

      化学と生物

      Volume: 50 Pages: 883-890

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 「おたまじゃくしの尾の消失―免疫学的な観点から見る動物の体作り」2012

    • Author(s)
      井筒 ゆみ
    • Journal Title

      化学と生物

      Volume: 50 Pages: 883-890

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Dmrt1 controls germ cell fate in Xenopus developing gonads2013

    • Author(s)
      Mawaribuchi S., Izutsu Y., Fujitani K., Komiya T., Takamatsu N., Ito M.
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、兵庫
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] ツメガエル性分化機構および脊椎動物雄化遺伝子Dmrt1の分子・機能進化2013

    • Author(s)
      伊藤道彦,回渕修治,藤谷和子,武蔵島正人,和田美加子,井筒ゆみ,高松信彦
    • Organizer
      第7回ツメガエル研究集会
    • Place of Presentation
      秋吉台国際芸術村、山口
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 両生類の器官形成における細胞死に働く免疫系(Immune system involved in the cell death during amphibian morphogenesis)2013

    • Author(s)
      井筒ゆみ
    • Organizer
      日本比較生理生化学会第35回大会
    • Place of Presentation
      イーグレひめじ,兵庫
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Knockdown of ouro genes represses tadpole tail regression during Xenopus metamorphosis(オウロ遺伝子のノックダウンは変態期のツメガエル幼生の尾部退縮を抑制する)2013

    • Author(s)
      Maruyama E., Maeno M., Izutsu Y.
    • Organizer
      第46回日本発生生物学会(APDBN共催)
    • Place of Presentation
      くにびきメッセ、島根
    • Related Report
      2013 Final Research Report
  • [Presentation] 両生類の器官形成における細胞死に働く免疫系2013

    • Author(s)
      井筒 ゆみ
    • Organizer
      日本比較生理生化学会第35回大会
    • Place of Presentation
      イーグレひめじ, 兵庫
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Knockdown of ouro genes represses tadpole tail regression during Xenopus metamorphosis2013

    • Author(s)
      Maruyama E., Maeno M., Izutsu Y.
    • Organizer
      第46回日本発生生物学会 (APDBN共催)
    • Place of Presentation
      くにびきメッセ、島根
    • Related Report
      2013 Annual Research Report
  • [Presentation] Immune antigen proteins Ouro1 and Ouro2 for tadpole tail degeneration in Xenopus metamorphosis2012

    • Author(s)
      Izutsu Y., Kikuta T., Kazama Y., Maeno M.
    • Organizer
      14th International Xenopus Conference(国際学会)
    • Place of Presentation
      Giens peninsula, France
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Immune antigen proteins Ouro1 and Ouro2 for tadpole tail degeneration in Xenopus metamorphosis2012

    • Author(s)
      Izutsu Y., Kikuta T., Kazama Y., Maeno M.
    • Organizer
      14th International Xenopus Conference
    • Place of Presentation
      Giens peninsula, France
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks] 科学雑誌Newton別冊「生き物の超能力」2012年9月掲載(pp30-31)

    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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