Analyses of the molecular interaction between epithelial organogenesis and immune system via specific antigens
| Project/Area Number |
24657086
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Niigata University |
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Principal Investigator |
IZUTSU YUMI 新潟大学, 自然科学系, 准教授 (20301921)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 発生・分化 / 免疫学 / 蛋白質 / 発現制御 / 動物 / アフリカツメガエル / 両生類の変態 / 幼生抗原 / 免疫 / 細胞死 / 皮膚 / T細胞 / タンパク質 |
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| Research Abstract |
We previously found a novel protein, named Ouro, as a candidate for immune antigens from amphibian. In this study, to clarify molecular mechanisms underlying the body plan of vertebrate animals, we focused on the functions of acquired immune system vs specific self-antigens, i.e., Ouro proteins. First, we examined immune cell response to the self-organ that expresses Ouro proteins. Using our transgenic Xenopus line, we found that immune T cells immediately migrated into Ouro expressing cells in the tadpole tail by using a live-cell-tracking. Second, we obtained polyclonal rat antisera against Ouro proteins. Third, using these antisera, we have tried the immunohistochemical analyses, and shown that a unique intracellular localization was seen when the Ouro proteins are recognized by immune cells.
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Report
(3 results)
Research Products
(11 results)
