| Project/Area Number |
24657087
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
KOJIMA Seiji 名古屋大学, 理学(系)研究科(研究院), 准教授 (70420362)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
HOMMA Michio 名古屋大学, 大学院理学研究科, 教授 (50209342)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 細菌べん毛 / モーター / 膜タンパク質 / 構造変化 |
|---|
| Research Abstract |
The PomA/PomB complex, that functions as an energy-converting stator unit in the bacterial flagellar motor, becomes functional when installed into the appropriate place around the rotor. In the functional stator, it is believed that a conformational change in the periplasmic region of PomB is induced to anchor it and simultaneously to activate ion conduction activity of the stator. This study aims to detect such a conformational change using the fluorescent probe and the purified PomA/B complex reconstituted into the liposome. We are still on the way to establish the analyzing system, but we greatly improved the overproduction and purification procedure for the PomA/B complex, so that we could systematically screen conditions to reconstitute the functional stator complex to the liposome. We also succeeded in narrowing down the region where the conformational change occurs, by using the systematic disulfide bridge formation analysis.
|
