In vitro reconstitution of intracellular copper transport system
| Project/Area Number |
24657093
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | タンパク質 / 分子認識 / 銅イオン / 金属シャペロン / 蛋白質 |
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| Research Abstract |
A copper ion is one of the metal ions essential for our life and often functions as an active center of enzymatic reactions in various proteins. Despite this, a free copper ion can be toxic because of its high redox activity that catalytically produces reactive oxygen species. Intracellular copper ions are hence necessary to be well regulated in terms of their localization and amounts. In this study, I have focused upon the intracellular pathway for transferring a copper ion to the copper-containing enzyme, SOD1. An extracellular copper ion has been known to enter into the cell via a copper transporter, CTR1; a copper chaperone, CCS, then delivers the copper ion to SOD1. The ultimate goal of this study is to reconstitute the copper transfer system in a test tube by using CTR1, CCS, and SOD1.
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Report
(3 results)
Research Products
(29 results)
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[Journal Article] Intranuclear aggregation of mutant FUS/TLS as a molecular pathomechanism of amyotrophic lateral sclerosis2014
Author(s)
Nomura, T., Watanabe, S., Kaneko, K., Yamanaka, K., Nukina, N. & Furukawa, Y.
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Journal Title
J. Biol. Chem.
Volume: 289
Issue: 2
Pages: 1192-1202
DOI
Related Report
Peer Reviewed / Open Access
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