| Project/Area Number |
24657099
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University |
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Principal Investigator |
HOMMA Michio 名古屋大学, 理学(系)研究科(研究院), 教授 (50209342)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYANOIRI Yohei 名古屋大学, 大学院理学研究科, 助教 (80547521)
KOJIMA Chojiro 大阪大学, たんぱく質研究所, 准教授 (50333563)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | べん毛 / NMR / タンパク質の構造 |
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| Research Abstract |
Compared with the proton-driven flagellar motor, it is believed that even in the sodium-driven flagellar motor, the C-terminal region of a rotor component FliG has important residues to produce the rotational force, however, the conserved charged residues did not give a critical result in sodium-driven motor. It has not been clarified to identify the key residues involved in the interaction directly between the residues of rotor and stator. In this study, the structure of C-terminal region of FliG was determined at 95% using NMR to show the interaction with the rotor directly and to assign the important interaction residues of FliG.
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