| Project/Area Number |
24657104
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
NAMBA KEIICHI 大阪大学, 生命機能研究科, 教授 (30346142)
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| Research Collaborator |
FUJII Takashi 理化学研究所, 生命システム研究センター, 基礎特別研究員 (10582611)
KATO Takayuki 大阪大学, 生命機能研究科, 助教 (20423155)
KAJIMURA Naoko 日本電子株式会社, 嘱託職員 (40403175)
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| Project Period (FY) |
2012-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 極低温電子顕微鏡法 / グラフェン / 蛋白質 / 生体分子 / 構造生物学 / 低温電子顕微鏡法 |
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| Research Abstract |
We tried to use a graphene sheet on a holey carbon grid as a specimen substrate for electron microscopy to develop a method to control the thickness of vitreous ice film in which frozen-hydrated particles of protein complexes are embedded. The specimen grids produced and provided by a company upon our specific request, however, were not really useful because many flakes of graphene were piled up to one another. We therefore made ultra thin carbon sheets as a temporary substitute for graphene, collected electron cryomicrographs of Shigella needle complex particles, and obtained a high-resolution image of its side view as a class average.
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