Technical development for high-resolution electron cryomicroscopy by utilizing graphene as a specimen substrate
| Project/Area Number |
24657104
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
NAMBA KEIICHI 大阪大学, 生命機能研究科, 教授 (30346142)
|
|---|
| Research Collaborator |
FUJII Takashi 理化学研究所, 生命システム研究センター, 基礎特別研究員 (10582611)
KATO Takayuki 大阪大学, 生命機能研究科, 助教 (20423155)
KAJIMURA Naoko 日本電子株式会社, 嘱託職員 (40403175)
|
|---|
| Project Period (FY) |
2012-04-01 – 2013-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
|
|---|
| Keywords | 極低温電子顕微鏡法 / グラフェン / 蛋白質 / 生体分子 / 構造生物学 / 低温電子顕微鏡法 |
|---|
| Research Abstract |
We tried to use a graphene sheet on a holey carbon grid as a specimen substrate for electron microscopy to develop a method to control the thickness of vitreous ice film in which frozen-hydrated particles of protein complexes are embedded. The specimen grids produced and provided by a company upon our specific request, however, were not really useful because many flakes of graphene were piled up to one another. We therefore made ultra thin carbon sheets as a temporary substitute for graphene, collected electron cryomicrographs of Shigella needle complex particles, and obtained a high-resolution image of its side view as a class average.
|
Report
(3 results)
Research Products
(68 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Structural analysis of the flagellar hook-basal body with the C ring by electron cryomicroscopy2013
Author(s)
Miyata, T., Kato, T., Fujii, T., Nakamura, S., Morimoto, Y.V., Minamino, T., Matsunami, H. and Namba, K.
Organizer
Nagoya Symposium -Frontiers in Structural Physiology-
Place of Presentation
Nagoya University(愛知県・名古屋市)
Related Report
-
-
-
[Presentation] Structural analysis of 100S ribosomes in two different types of bacteria2013
Author(s)
Kato, T., Ueta, M., Miyata, T., Wada, C., Yoshida, H., Wada, A. and Namba, K.
Organizer
Nagoya Symposium -Frontiers in Structural Physiology-
Place of Presentation
Nagoya University(愛知県・名古屋市)
Related Report
-
-
-
-
-
-
-
-
-
[Presentation] 飢餓的ストレス下での100Sリボソーム形成による細菌の生存戦略. Survival strategy of bacteria under the starvation stress by 100S ribosome formation2012
Author(s)
Kato, T., Ueta, M., Miyata, T., Yoshica, H., Wada, A., Namba, K.
Organizer
第50回日本生物物理学会年会
Place of Presentation
名古屋大学(愛知県・名古屋市)
Year and Date
2012-09-24
Related Report
-
-
[Presentation] 細菌べん毛モーターの回転方向変換制御機構の解明. Elucidation of the directional switching mechanism of the bacterial flagellar motor2012
Author(s)
Miyata, T., Kato, T., Fujii, T., Nakamura, S., Morimoto, Y., Minamino, T., Matsunami, H., Namba, K.
Organizer
第50回日本生物物理学会年会
Place of Presentation
名古屋大学(愛知県・名古屋市)
Year and Date
2012-09-23
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
