| Project/Area Number |
24657107
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KUBO Minoru 独立行政法人理化学研究所, 放射光科学総合研究センター, 専任研究員 (90392878)
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| Research Collaborator |
NAGATOMO Shigenori 筑波大学, 数理物質系化学域, 講師 (80373190)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 揺らぎ / 振動コヒーレンス / ヘモグロビン / タンパク質 / 低振動 / フェムト秒レーザー / 生体分子分光 |
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| Outline of Final Research Achievements |
Vibrational coherence spectroscopy was applied to study the correlation between fluctuation of heme and its O2 affinity in hemoglobin (Hb). Damping times of low-frequency coherences (<200 cm-1) of heme, including doming mode coherence, were compared between 5 deoxyHbs with different O2 affinities, regulated by heterotropic effectors. The results showed that the damping times were not correlated with the O2 affinities. Instead, it was confirmed that the Fe-His stretching frequencies were correlated with the O2 affinities. Thus, this study supports Perutz model for the Hb function, although the role of fluctuation of the O2 channel remains to be examined.
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