Optical and electron microscopic analyses on arrangements of bacterial membrane protein complexes
| Project/Area Number |
24657108
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Hosei University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Kazunori 国立研究開発法人産業技術総合研究所, 生命工学領域・バイオメディカル研究部門, 主任研究員 (40356837)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 細胞膜 / 膜蛋白質 / 大腸菌 / 蛍光顕微鏡 / 電子顕微鏡 / 凍結割断レプリカ法 / 光学顕微鏡 / 蛋白質 |
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| Outline of Final Research Achievements |
In this study, we aimed at identifying intra-membrane particles of Escherichia coli and characterizing their localization, arrangement and their regulation in response to environmental changes. We focused on chemoreceptors, which form a huge cluster at a pole of the inner membrane, and xenobiotic efflux complexes, which bridge the inner and the outer membranes. We obtained valuable findings concerning localization, membrane dynamics and cluster structure of chemoreceptors using improved protocols of fluorescence microscopic observations. Quick-freezing replica and ultra-thin section electron microscopic analyses were also carried out to characterize chemoreceptors and xenobiotic efflux complexes.
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Report
(4 results)
Research Products
(9 results)
