| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this study, we aimed at identifying intra-membrane particles of Escherichia coli and characterizing their localization, arrangement and their regulation in response to environmental changes. We focused on chemoreceptors, which form a huge cluster at a pole of the inner membrane, and xenobiotic efflux complexes, which bridge the inner and the outer membranes. We obtained valuable findings concerning localization, membrane dynamics and cluster structure of chemoreceptors using improved protocols of fluorescence microscopic observations. Quick-freezing replica and ultra-thin section electron microscopic analyses were also carried out to characterize chemoreceptors and xenobiotic efflux complexes.
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